The present invention provides an artificial skin and a preparation method thereof. The present invention takes the xenogeneic acellular dermal matrix particles as main materials, and obtains the dermis layer by three-dimensional printing technologies, and then obtains the artificial skin by combining the epidermis layer with the dermis layer. The dermis layer of artificial skin in present invention has three-dimensional porous structure, which retains main components of natural dermal matrix in composition, and imitates distributed structure at fiber bundle diameter and pore size of natural dermal matrix in structure. This kind of novel biomimetic dermal scaffolds have obvious advantages in inducing migration and regeneration of skin cells, accelerating vascularization, promoting wound healing and improving healing quality. The dermis layer of artificial skin in present invention is obtained by three-dimensional printing technologies, which has precise and controllable structure, simple preparation method and high products qualification rate.

A bone regeneration material has a cotton-wool like structure formed of a plurality of electrospun fibers that contain bound BMP-2 through β-TCP binding peptide. The electrospun biodegradable fiber contains 25-65 vol % of β-TCP particles distributed in the fiber such that a portion of the β-TCP particles is exposed on a surface of the electrospun fiber and the remaining portion of the β-TCP particles is buried in the fiber. β-TCP binding peptides that are fused with BMP-2 are bound to the β-TCP particles so that BMP-2 is tethered to β-TCP particles on the surface of the fibers. Upon implantation of the bone regeneration material in a bone defect site of a human body, BMP-2 that are tethered to β-TCP particles on the surface of the bone regeneration material promotes proliferation and differentiation of cells at the bone defect site.

The present invention relates to porous composite materials and objects such as 3D scaffolds, in particular to bioactive and bioresorbable scaffolds that can be transformed at body temperature.

A method for controlling generation of biologically desirable voids in a composition placed in proximity to bone or other tissue in a patient by selecting at least one water-soluble inorganic material having a desired particle size and solubility, and mixing the water-soluble inorganic material with at least one poorly-water-soluble or biodegradable matrix material. The matrix material, after it is mixed with the water-soluble inorganic material, is placed into the patient in proximity to tissue so that the water-soluble inorganic material dissolves at a predetermined rate to generate biologically desirable voids in the matrix material into which bone or other tissue can then grow.

A method for producing a composite demineralized bone matrix composition using a one-step process is described. The composite demineralized bone matrix composition is produced from the biologically-derived bone. In addition, the composite demineralized bone matrix composition contains bone minerals according to the original composition proportion in the bone and may provide a bone mineral content condition that is closest to that in an environment in which in vivo bone formation occurs. In addition, the composition contains a bone morphogenetic protein (BMP-2), and thus enables a stable and excellent bone formation effect to be derived.

A method for producing a composite demineralized bone matrix composition using a one-step process is described. The composite demineralized bone matrix composition is produced from the biologically-derived bone. In addition, the composite demineralized bone matrix composition contains bone minerals according to the original composition proportion in the bone and may provide a bone mineral content condition that is closest to that in an environment in which in vivo bone formation occurs. In addition, the composition contains a bone morphogenetic protein (BMP-2), and thus enables a stable and excellent bone formation effect to be derived.

A composite implant comprising an injectable matrix material which is flowable and settable, and at least one reinforcing element for integration with the injectable matrix material, the at least one reinforcing element adding sufficient strength to the injectable matrix material such that when the composite implant is disposed in a cavity in a bone, the composite implant supports the bone. A method for treating a bone, the method comprising: selecting at least one reinforcing element to be combined with an injectable matrix material so as to together form a composite implant capable of supporting the bone; positioning the at least one reinforcing element in a cavity in the bone; flowing the injectable matrix material into the cavity in the bone so that the injectable matrix material interfaces with the at least one reinforcing element; and transforming the injectable matrix material from a flowable state to a non-flowable state so as to establish a static structure for the composite implant, such that the composite implant supports the adjacent bone.

Artificial cartilage materials for repair and replacement of cartilage, such as load-bearing and articular cartilage. The artificial cartilage materials can include a hydrogel with an internal polymer support network that impart the hydrogel mechanical properties similar to that of natural cartilage. In some examples, the hydrogels include a cross-linked cellulose network and a double network of polyvinyl alcohol (PVA) and polyacrylamide-methyl propyl sulfonic acid (PAMPS) polymers. The hydrogels may include specific formulations of different polymers to impart mechanical properties that are within a cartilage equivalent range. The artificial cartilage materials may include a porous base that is bonded to the hydrogel for interfacing with surrounding tissues and promoting ingrowth of bone and/or cartilage. Thus, the materials may be well suited for forming a synthetic graft, such as an osteochondral graft, for implantation into a patient's body.

Artificial cartilage materials for repair and replacement of cartilage, such as load-bearing and articular cartilage. The artificial cartilage materials can include a hydrogel with an internal polymer support network that impart the hydrogel mechanical properties similar to that of natural cartilage. In some examples, the hydrogels include a cross-linked cellulose network and a double network of polyvinyl alcohol (PVA) and polyacrylamide-methyl propyl sulfonic acid (PAMPS) polymers. The hydrogels may include specific formulations of different polymers to impart mechanical properties that are within a cartilage equivalent range. The artificial cartilage materials may include a porous base that is bonded to the hydrogel for interfacing with surrounding tissues and promoting ingrowth of bone and/or cartilage. Thus, the materials may be well suited for forming a synthetic graft, such as an osteochondral graft, for implantation into a patient's body.

A medical implant comprising a plurality of layers, each layer comprising a polymer and a plurality of uni-directionally aligned continuous reinforcement fibers.