In alternative embodiments, provided are compositions, e.g., pharmaceutical compositions, formulations, kits and other products of manufacture, comprising a hydrogel and active ingredients, including mixed thickness skin micrografts, or full or split-thickness skin grafts, contained or mixed in or within the hydrogel; and methods for making and using them. In alternative embodiments, compositions and methods as provided herein are used for the treatment or amelioration of wounds and surgical sites, and include compositions and methods for micrografting, or for micrografting a wound, or for micrografting a wound for rapid re-epithelialization, or for micrografting a wound for rapid re-epithelialization of large non-healing wounds.

In alternative embodiments, provided are compositions, e.g., pharmaceutical compositions, formulations, kits and other products of manufacture, comprising a hydrogel and active ingredients, including mixed thickness skin micrografts, or full or split-thickness skin grafts, contained or mixed in or within the hydrogel; and methods for making and using them. In alternative embodiments, compositions and methods as provided herein are used for the treatment or amelioration of wounds and surgical sites, and include compositions and methods for micrografting, or for micrografting a wound, or for micrografting a wound for rapid re-epithelialization, or for micrografting a wound for rapid re-epithelialization of large non-healing wounds.

The present disclosure relates to tissue matrix products. The products can includes tissue matrices that have holes or perforations located at certain positions to improve certain in vivo functions without substantial loss of strength or other important properties.

Based on dimensions and configurations of a dermal wound on a patient, a carrier is custom-made, preferably by 3D printing, to include a cavity that is duplicative of the specific wound. When the custom-made carrier is used to contain slurry, and the slurry-containing carrier undergoes processing steps (such as freeze-drying in a lyophilization chamber), a matrix is formed that, when removed from the carrier and put into the wound of the patient for whom the carrier was custom-made, fills the wound volume.

Provided herein are methods of making a cell growth scaffold from adipose tissue, cell growth scaffolds having low lipid content and methods of using the cell growth scaffold.

Provided herein are methods of making a cell growth scaffold from adipose tissue, cell growth scaffolds having low lipid content and methods of using the cell growth scaffold.

A composition comprising a first material and a second material, wherein said first material is cross-linkable by a first cross-linking reaction and said second material is cross-linkable by a second cross-linking reaction, wherein said first cross-linking reaction and said second cross-linking reaction are inducible by a common activator.

A polymer nanofiber scaffold includes a plurality of melt extruded nanofibers that are chemically modified to append surface functionality to the nanofibers.

A polymer nanofiber scaffold includes a plurality of melt extruded nanofibers that are chemically modified to append surface functionality to the nanofibers.

A method for preparing a skin regeneration scaffold is disclosed. The method may include preparing a polymer solution by dissolving a biopolymer in a solvent, and subjecting the polymer solution to a template-assisted extrusion process with a nanoporous material as a template in order to produce polymer nanofibers. Furthermore, the method includes fabricating a multilayer composite nanofibrous scaffold using the polymer nanofibers. The composite nanofibrous scaffold may be seeded with cells. In some cases, the cells may be selected from autologous cells, allogeneic cells, or combinations thereof.