The present invention relates to a dermal layer which is for grafting and has an improved graft survival rate, and a method for producing the same, wherein the dermal layer for grafting can be produced by filling a filling solution, including a DNA fragment mixture and chitosan, into an acellular dermal matrix from which cells have been removed. It was observed that the dermal layer for grafting produced in this manner, due to the filling solution filled therein and including a DNA fragment mixture and chitosan, increases the rate at which cells flow in from the tissue surrounding the graft and are fixed, and thereby alleviates an initial inflammatory reaction and promotes blending with the surrounding tissue.

A process is described for the purification of HA, and pharmaceutical, cosmetic and nutritional compositions containing HA thus purified.

A process is described for the purification of HA, and pharmaceutical, cosmetic and nutritional compositions containing HA thus purified.

To provide means for efficient growth of epithelial cells capable of being used to manufacture regenerated hair follicle germs. Provided are a culture medium for growth of epithelial cells capable of being used to manufacture regenerated hair follicle germs, the culture medium comprising basal medium and at least (1) through (3), below: and a method for growing the epithelial cells using the culture medium: (1) at least one species of BMP inhibitor: (2) at least one species of fibroblast growth factor: and (3) at least one species of sonic hedgehog (SHH) and/or SHH agonist.

The present invention relates to a method for the production of a biocompatible matrix the method comprising: a) providing a composition comprising at least one biopolymer; b) extruding the composition into a layer through a slit onto a surface, wherein the slit moves over the surface; c) optionally freezing the layer; d) optionally repeating the process to add one or more further layers; e) freezing the composition or compositions after extrusion; f) optionally drying the frozen composition wherein preferably the surface can be cooled. The invention further relates to a biocompatible matrix obtainable by the method as well as to the use of a matrix obtainable by the method for medical or cosmetic purposes. Moreover, the present invention also relates to a device for the production of a biocompatible matrix, comprising: a) a temperature controlled surface; b) an extruding means with a slit positioned above the surface; c) means for extruding a composition through the slit; d) means for moving the slit and/or the surface.

The present invention relates to a method for the production of a biocompatible matrix the method comprising: a) providing a composition comprising at least one biopolymer; b) extruding the composition into a layer through a slit onto a surface, wherein the slit moves over the surface; c) optionally freezing the layer; d) optionally repeating the process to add one or more further layers; e) freezing the composition or compositions after extrusion; f) optionally drying the frozen composition wherein preferably the surface can be cooled. The invention further relates to a biocompatible matrix obtainable by the method as well as to the use of a matrix obtainable by the method for medical or cosmetic purposes. Moreover, the present invention also relates to a device for the production of a biocompatible matrix, comprising: a) a temperature controlled surface; b) an extruding means with a slit positioned above the surface; c) means for extruding a composition through the slit; d) means for moving the slit and/or the surface.

The present invention provides for methods and devices suitable for producing a transplantable cellular suspension of living tissue suitable for promoting tissue regeneration in an epithelium-related procedure, as well as compositions produced therefrom. The cellular suspension can include viable and functioning cells at various stages of differentiation, including undifferentiated/progenitor cells and differentiated cells, as well as those in between. In certain embodiments, the cellular suspension can be subjected to a stress to induce a heat shock response therein, or be exposed to an exogenously supplied agent such as heat shock protein or a fragment thereof, hyaluronic acid, platelet-enriched plasma, and/or growth factors. The cellular suspension can be applied directly to a patient's recipient site for in vivo regeneration, or be cultured or seeded to a matrix for in vitro growth/regeneration.

The present invention provides for methods and devices suitable for producing a transplantable cellular suspension of living tissue suitable for promoting tissue regeneration in an epithelium-related procedure, as well as compositions produced therefrom. The cellular suspension can include viable and functioning cells at various stages of differentiation, including undifferentiated/progenitor cells and differentiated cells, as well as those in between. In certain embodiments, the cellular suspension can be subjected to a stress to induce a heat shock response therein, or be exposed to an exogenously supplied agent such as heat shock protein or a fragment thereof, hyaluronic acid, platelet-enriched plasma, and/or growth factors. The cellular suspension can be applied directly to a patient's recipient site for in vivo regeneration, or be cultured or seeded to a matrix for in vitro growth/regeneration.

Systems and methods for assembling a plurality of tissue grafts are provided. A method includes applying a magnetic coating over a surface of a donor site and harvesting the plurality of micro tissue grafts from the donor site, so that an upper surface of each of the plurality of micro tissue grafts contains the coating. The method also includes arranging a magnet over the magnetic coating to induce the plurality of micro tissue grafts to organize in a desired orientation, forming a tissue construct containing the plurality of micro tissue grafts arranged in the desired orientation, and applying the tissue construct to a recipient site.

Systems and methods for assembling a plurality of tissue grafts are provided. A method includes applying a magnetic coating over a surface of a donor site and harvesting the plurality of micro tissue grafts from the donor site, so that an upper surface of each of the plurality of micro tissue grafts contains the coating. The method also includes arranging a magnet over the magnetic coating to induce the plurality of micro tissue grafts to organize in a desired orientation, forming a tissue construct containing the plurality of micro tissue grafts arranged in the desired orientation, and applying the tissue construct to a recipient site.