The invention relates to a fully disposable system for casting, polymerizing and compressing a hydrogel. The invention further relates to a method for producing a scaffold for the generation of artificial tissue products using said disposable system.

Provided subject matter relates to tissue engineering. More specifically provided are kits, devices and methods for in situ repair and regeneration of guided and functional growth of cells and cell components by providing into the injury site biomaterial solution including the cell(s), magnetic particles and solidifying the biomaterial while applying the magnetic field.

Provided subject matter relates to tissue engineering. More specifically provided are kits, devices and methods for in situ repair and regeneration of guided and functional growth of cells and cell components by providing into the injury site biomaterial solution including the cell(s), magnetic particles and solidifying the biomaterial while applying the magnetic field.

A polypeptide monolayer with a high surface potential and hydrophobicity, and a preparation method and application thereof. The polypeptide is composed of polypeptide molecules with a molecular weight of (1.48±0.2)×10.sup.5 g/mol, a thickness of the monolayer is 17.3-18.5 nm, the exposure of primary amino groups on the surface of the monolayer is 11-11.8%, a Zeta potential of the polypeptide monolayer is (-3)-(-2) mV, and a contact angle of the monolayer is 84±1°. A micro-nano structure on the surface of the polypeptide monolayer allows the polypeptide monolayer to have certain hydrophobicity, which is beneficial to water proofing of the surface of biomimetic skin. Furthermore, the surface of the polypeptide monolayer has a relatively high potential, which can improve biocompatibility, hemocompatibility, and cell adhesion, proliferation, and differentiation abilities.

A polypeptide monolayer with a high surface potential and hydrophobicity, and a preparation method and application thereof. The polypeptide is composed of polypeptide molecules with a molecular weight of (1.48±0.2)×10.sup.5 g/mol, a thickness of the monolayer is 17.3-18.5 nm, the exposure of primary amino groups on the surface of the monolayer is 11-11.8%, a Zeta potential of the polypeptide monolayer is (-3)-(-2) mV, and a contact angle of the monolayer is 84±1°. A micro-nano structure on the surface of the polypeptide monolayer allows the polypeptide monolayer to have certain hydrophobicity, which is beneficial to water proofing of the surface of biomimetic skin. Furthermore, the surface of the polypeptide monolayer has a relatively high potential, which can improve biocompatibility, hemocompatibility, and cell adhesion, proliferation, and differentiation abilities.

Some embodiments are directed to a method for preparing a skin substitute, a dermal substitute, to a skin substitute, to a dermal substitute and to a kit for implementing the method. Some other embodiments are directed to a graft that can consist of of a skin substitute and to the use thereof as treating a skin disorder and/or a loss of skin substance.

Some embodiments are directed to a method for preparing a skin substitute, a dermal substitute, to a skin substitute, to a dermal substitute and to a kit for implementing the method. Some other embodiments are directed to a graft that can consist of of a skin substitute and to the use thereof as treating a skin disorder and/or a loss of skin substance.

Disclosed are bioprinted, three-dimensional, biological skin tissues comprising: a dermal layer comprising dermal fibroblasts; and an epidermal layer comprising keratinocytes, the epidermal layer in contact with the dermal layer to form the three-dimensional, engineered, biological skin tissue. Also disclosed are arrays of engineered skin tissues and methods of making engineered skin tissues.

Disclosed are bioprinted, three-dimensional, biological skin tissues comprising: a dermal layer comprising dermal fibroblasts; and an epidermal layer comprising keratinocytes, the epidermal layer in contact with the dermal layer to form the three-dimensional, engineered, biological skin tissue. Also disclosed are arrays of engineered skin tissues and methods of making engineered skin tissues.

The present disclosure provides tissue products produced from extracellular tissue matrices. The tissue products can include acellular extracellular matrices including combinations of different tissue types. The combination can harness various properties of the different tissues to provide improved composite structures with desired mechanical and/or biologic properties.