A laser is used to form openings within a graft material to selectively enhance permeability of a prosthesis for tissue integration therein. A feature of utilizing a laser to create the openings for tissue integration builds from its tunability. More particularly, the laser precisely places openings in any pattern and location, and on any textile that forms the graft material. Further, the power and focus of the laser is precisely adjusted to control the diameter and shape of the openings. All parameters of the openings can be controlled at will, allowing for the opportunity to selectively enhance and optimize the permeability of the graft material in a vessel.

The techniques of this disclosure generally relate to a variable permeability layered prosthesis including an impermeable outer layer and a permeable inner layer. The impermeable outer layer is well suited to seal a dissection opening of a dissection. The permeable inner layer allows fluid to enter into a dead space between the impermeable outer layer and the permeable inner layer. The fluid in the dead space coagulates in the dead space providing a media for tissue growth into the prosthesis. The ability of tissue to integrate into the prosthesis provides biological fixation of the prosthesis in vessels and prevents endoleaks and migration of the prosthesis.

The invention provides polymer compositions for cell and drug delivery.

Methods and compositions are described herein that promote bone formation. Such methods and compositions include SLIT3 or SLIT2 agents that can be administered to a subject (e.g., one in need thereof). Methods are also described herein that reduce or prevent unwanted bone formation. Such methods can involve administering an inhibitor of SLIT3 or SLIT2 to a subject.

The present invention relates to a composition of a calcium phosphate such as tetracalcium phosphate or -TCP and an additive compound selected from nucleic acid or nucleotides, phospho(enol)pyruvic acid and phosphocreatine. The composition may be used as a tissue adhesive.

A needle lattice is used to form openings within a graft material to selectively enhance permeability of a prosthesis for tissue integration therein. The needle lattice may be disposed on, for example, a surface of a roller or press. The needle lattice precisely places openings in any pattern and location, and on any textile that forms the graft material. The needle lattice can be heated to fuse the surrounding material of the openings of the textile to prevent movement of the textiles and to prevent collapse of the openings. All parameters of the openings, including varying density, patterns, and size of each opening, can be controlled, allowing for the opportunity to selectively enhance and optimize the permeability of the graft material in a vessel. The needle lattice can quickly form multiple openings within a graft material, allowing for quick manufacturing of the prosthesis.

The present disclosure provides click-crosslinked hydrogels and methods of use.

Porous implantable devices for housing one or more therapeutic agents are disclosed herein. The implantable devices include a porous outer wall defining an interior void. The interior void houses a carrier material carrying a first therapeutic agent. The implantable devices are made by patterning at least a portion of a polymerizable substrate into a polymerized three-dimensional porous outer wall surrounding an interior void. This can be achieved by two-photon polymerization techniques. A first therapeutic agent is then added to the interior void, which is then sealed. Methods of treating diseases using the implantable devices are disclosed herein. The methods include implanting the implantable device at a target area and locally releasing a therapeutically effective dosage of a first therapeutic agent from the interior void. The implantable devices can also be used in methods of screening potentially therapeutic agents for desired biological responses.

The present invention relates to enhancing mechanical properties of tissue such as collagenous or collagen-containing or elastin-containing tissue (e.g., tendons, ligaments, and cartilage) and treating related musculoskeletal and non-musculoskeletal conditions or injuries.

The invention provides a novel, versatile degradable hydrogel composition, and methods thereof, with precisely tunable stiffness, plasticity (e.g., degree of covalent vs. physical crosslinks) and predictive disintegration rates degradation, allowing controlled disintegration and release of therapeutic cells or pharmaceuticals and/or in vitro 3D cell expansion.