Embodiments provide methods and apparatus for fabricating blood products, such as platelet-rich plasma, containing elevated concentrations of growth factors such as platelet derived growth factor. The platelet-rich plasma can be autologous, and the concentration of growth factors (e.g., platelet derived growth factor) is elevated relative to other samples isolated from the same subject. The platelet-rich plasma can be used to promote tissue regeneration, including wound healing, joint repair, hair growth, and the like. The compositions can be combined with stem cells and used to treat disorders otherwise treated with stem cells. The growth factors present in the samples can direct the differentiation of the stem cells.

A tray for processing platelet rich fibrin includes a base having at least one alignment structure, a screen attachment having at least one alignment structure for engagement with the base, and a lid having at least one alignment structure for engagement with the base and the screen attachment. The screen attachment includes a screen offset between the top and bottom surface of the screen. When the screen attachment is placed on the base with the top surface facing upward, the lid is configured to compress a fibrin clot placed on the screen to a first thickness, and when the screen attachment is placed on the base with the bottom surface facing upward the lid is configured to compress a fibrin clot placed on the screen to a second thickness different from the first thickness.

A system for blood cell separation, comprising:

a separation chamber (10) comprising an inlet port for blood (12), an outlet port for plasma (14) and at least an outlet port for cellular blood components (16) for the separation of whole blood;

a blood pump (20) for pumping whole blood into the inlet port for blood (12);

a plasma pump (22) for pumping plasma and/or target cells from the outlet port for plasma (14) out of the separation chamber (10);

a red blood cell tube (30) comprising a first end (32) and a second end (34), wherein the first end (32) of the red blood cell tube (30) is connected to the outlet port for cellular blood components (16) for allowing red blood cells to leave the separation chamber (10); and

a drip chamber (40) comprising a reservoir (42) and an inlet (46), wherein the second end (34) of the red blood cell tube (30) is connected to the inlet (46), wherein the second end (34) of the red blood cell tube (30) extends into the volume of the reservoir (42) for pressure equalization during pumping from the outlet port for plasma (14).

The present invention relates to: a combination therapy and a combination product that are for the mobilization of a large amount of hematopoietic stem cells in blood and include substance-P and AMD3100 or a pharmaceutically acceptable salt thereof; a method for collecting a large amount of mobilized hematopoietic stem cells, the method comprising a step for isolating a blood fraction containing the mobilized hematopoietic stem cells from peripheral blood obtained from an individual sequentially administered with substance P and AMD3100 or a pharmaceutically acceptable salt thereof; and a use of a combined product containing substance P and AMD3100 or a pharmaceutically acceptable salt thereof for the mobilization of a large amount of hematopoietic stem cells in blood.

An independent blood filter device depends on flow geometry to deliver blood serum or plasma free of detrimental levels of hemoglobin. It depends critically on an upstream flow rate or pressure differential limiting control element or device that limits the rate of change of pressure differential across the filter element. Pre-evacuated versions can be used to simultaneously draw blood from a living being and provide pressure differential across the filter element between an evacuated collector and a supply end open to atmosphere. A unit pressurized by hand motion employs the external shape of a partially filled blood collection tube as a piston to produce pressure in advance of the control element or device to create the pressure differential across the filter element to a collector vented to atmosphere. The control element or device is disclosed in numerous forms, including specially sized flow constrictions and compliant arrangements.

Mixing device (1) and fluid mixing method, by means of successive transfers between syringes. The mixing method comprises placing a pair of syringes (2, 3) in the mixing device (1), adjusting a variable fixing element adaptable to the pair of syringes (2, 3) and selecting and running a mixing programme, being the mixing force, the speed and the range and the number of transfers adjustable. The mixing device comprises a mobile carriage (7) longitudinally movable and fixing elements (8, 9) with an adjustable distance between them. The invention allows for the mixing or emulsifying of blood fluids with different viscosity, particularly a protein gel and a platelet-rich plasma, for the preparation of dermatological formulations, in a versatile, hygienic and effective manner.

Disclosed are methods, materials and devices for approximation of blood volume in a fluid, such as in a biological fluid collected during a surgical procedure. The method and devices include the use of a RBC flocculant, such as polyDADMAC, and an approximate blood hematocrit for the type of animal, as well as a calculated RBC packing ratio corresponding to the collection device being used. Also provided is a Blood Indicator Panel (BIP), comprising a series of markings calculated from an observed red blood settlement volume, the average animal type hematocrit, and a calculated RBC packing ratio “η” value for the collection device. Pediatric (about 200 ml or 250 ml size container), adult human (about 1,000 ml-1,500 ml) and veterinary (about 500 ml-2,500 ml) collection containers are also disclosed, that include a RBC flocculant, for use in approximating blood volume in a fluid.

A system to be used to treat Leukemia, comprises a device to power, control and monitor an ultraviolet radiation as well as to monitor a blood contactless conductivity; and a catheter assembly with an inner dialysis catheter, an ultraviolet radiation source and a differential coil sensor. The system is using wavelengths of ultraviolet radiation to destroy infectious microbes that are in the blood and that are inside circulating tumor cells directly and indirectly as well as to destroy infectious microbes at primary tumor location (bone marrow). This is done without withdrawing the blood out of the body. Furthermore, the system measures a blood conductivity to evaluate the treatment process in a real time during the operation.

Disclosed are methods, materials and devices for approximation of blood volume in a fluid, such as in a biological fluid collected during a surgical procedure. The method and devices include the use of a RBC flocculant, such as polyDADMAC, and an approximate blood hematocrit for the type of animal, as well as a calculated RBC packing ratio corresponding to the collection device being used. Also provided is a Blood Indicator Panel (BIP), comprising a series of markings calculated from an observed red blood settlement volume, the average animal type hematocrit, and a calculated RBC packing ratio “η” value for the collection device. Pediatric (about 200 ml or 250 ml size container), adult human (about 1,000 ml-1,500 ml) and veterinary (about 500 ml-2,500 ml) collection containers are also disclosed, that include a RBC flocculant, for use in approximating blood volume in a fluid.

Embodiments of the invention include instruments and methods useful in preparing and delivering graft material to a surgical site. Some embodiments may particularly be directed to forming a graft from a blood clot and accurately and effectively handling and delivering the graft to a surgical site. Graft material may include blood components such as clotted fibrin derived from a patient's or a donor's blood.