A medicine-holding body is disposed in a flow path of a nozzle portion of an adapter for blood dispensing. The medicine-holding body is formed of a plurality of fibers which is made of polyester and is bundled by aligning a longitudinal direction thereof in a flowing direction which is a direction in which blood flows. The medicine-holding body holds an anticoagulant for suppressing coagulation of blood, as a medicine to be mixed into blood. The surface area of the medicine-holding body is greater than or equal to 10 mm.sup.2 and less than 600 mm.sup.2. In a case where the surface area of the medicine-holding body is greater than or equal to 10 mm.sup.2, the concentration of the anticoagulant becomes greater than or equal to a lower limit value of 10 U/mL even under most severe conditions such as a dispensing speed of 500 L/second. In a case where the surface area of the medicine-holding body is less than 600 mm.sup.2, it is possible to maintain the occurrence rate of hemolysis to be less than or equal to 10%.

A centrifugal separation device of a blood component collection system comprises a data acquisition unit that acquires initial data A, an estimated data calculation unit that calculates estimated data B on the basis of the initial data A, a reaction force calculation unit which calculates a reaction force of a first applied load measurement unit on the basis of the estimated data B, and an internal pressure calculation unit that calculates an internal pressure of the first applied load measurement unit, based on the reaction force and a load detected by a first load detecting unit.

Disclosed is a multiple bag system for fractionating blood, the system including a fluid collecting bag including at least one outlet port; at least first and second sampling bags, each including at least one inlet port and at least one outlet port; and a fluid transfer unit to transfer fluid from the collecting bag to the sampling bags. The fluid transfer unit includes an acoustic sorter. Also disclosed is a method for fractionating blood into blood products.

An apparatus for isolating stems cells from extracted mammalian tissue comprising: a portable hollow casing having fixed dimensions and a sized internal spatial volume; a filter housed and contained within said sized internal spatial volume, wherein said filter captures particles in said extracted mammalian tissue having a diameter of about 5 to 10 microns or more and allows particles in said extracted mammalian tissue having a diameter of less than about 5 to 10 microns to pass through; a first channel to which a container holding said extracted mammalian tissue can attach, and through which said extracted mammalian tissue is input into the hollow casing; wherein a stem cell collection chamber can attach to said first channel, and the particles having a diameter of about 5 to 10 microns or more are output from the hollow casing through said first channel and collected in the stem cell collection chamber; and a second channel to which a remnant collection chamber can attach, and through which the particles having a diameter of less than about 5 to 10 microns are output from the hollow casing and collected in the remnant collection chamber.

Methods of preparing liquid exchanged biological fractions are provided. Aspects of the present disclosure according to certain embodiments include introducing a biological sample into a centrifugation container having a buoy, subjecting the biological sample to a force of centrifugation to produce two or more fractions in the biological sample, collecting a first fraction of the biological sample from the container and introducing a liquid exchange fluid into the container to produce a liquid exchanged fraction that is a mixture of the liquid exchange fluid and a second fraction of the biological sample. Compositions having a liquid exchanged platelet-rich fluid and kits for practicing embodiments of the subject methods are also provided. Methods for applying compositions having a liquid exchanged platelet-rich fluid to a body site of the subject are described.

Apparatus and methods for processing blood are disclosed in which one variation generally comprises a tube defining a channel and an access tube extending into the channel. An open cell matrix configured to entrap red blood cells may be positioned within at least a portion of the channel. Another variation generally comprises a cylindrical tube and a plunger slidably positioned within the channel. The plunger also has a funnel positioned upon the plunger and is movable therewith. Both the plunger and funnel define a fluid channel through and in communication with the cylindrical tube.

Mixing device (1) and fluid mixing method, by means of successive transfers between syringes. The mixing method comprises placing a pair of syringes (2, 3) in the mixing device (1), adjusting a variable fixing element adaptable to the pair of syringes (2, 3) and selecting and running a mixing programme, being the mixing force, the speed and the range and the number of transfers adjustable. The mixing device comprises a mobile carriage (7) longitudinally movable and fixing elements (8, 9) with an adjustable distance between them. The invention allows for the mixing or emulsifying of blood fluids with different viscosity, particularly a protein gel and a platelet-rich plasma, for the preparation of dermatological formulations, in a versatile, hygienic and effective manner.

The present disclosure provides cell therapy production systems that can suitably be used in a patient bedside setting. Such systems allow for direct removal of a patient's blood, automated processing to produce a cell therapy, and then infusion back into the patient, without the need to remove the system from the patient's bedside. Also provided herein are systems for production of cell therapies in a bedside setting.

Devices and methods are provided herein for exchange between a first agent and a second agent. The device includes a first chamber having a first inlet and a first outlet with a first flow passageway extending between the first inlet and the first outlet for flowing a first agent through the first chamber. The device also includes a second chamber having a second inlet and a second outlet with a second flow passageway extending between the second inlet and the second outlet for flowing a second agent through the second chamber and a membrane positioned between the first flow passageway and the second flow passageway to allow exchange through the membrane of the first agent with the second agent. Methods of nitrosylating blood and methods of manufacturing the device are also provided.

A centrifugal separation device of a blood component collection system comprises a data acquisition unit that acquires initial data A, an estimated data calculation unit that calculates estimated data B on the basis of the initial data A, a reaction force calculation unit which calculates a reaction force of a first applied load measurement unit on the basis of the estimated data B, and an internal pressure calculation unit that calculates an internal pressure of the first applied load measurement unit, based on the reaction force and a load detected by a first load detecting unit.