A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma/bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow/platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate/platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate/platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma/bone marrow concentrate fractions.

The present invention relates to a platelet rich plasma (PRP) extracting device and extracting method using the same, and more particularly, to a platelet rich plasma (PRP) extracting device capable of quickly and effectively extracting the highly concentrated PRP from centrifuged blood by a simple manipulation.

A blood component separation device for separating a plurality of blood components from blood sampled from a blood donor, and collecting platelets, includes: an operation unit that calculates a predicted platelet recovery rate from a hematocrit value of the blood and a platelet concentration of the blood, and calculates a recommended processing amount of the blood recommended for collecting a target number of units of platelets on the basis of the calculated predicted platelet recovery rate, wherein the operating unit sets the predicted platelet recovery rate calculated from any the hematocrit value and any the platelet concentration to be smaller by a predetermined value a when the blood donor is female than that when the blood donor is male.

The present disclosure provides compositions and methods for managing female infertility, caused by reduced thickness and receptivity of the endometrial lining. More particularly, the present disclosure provides a platelet derived growth factor concentrate and a composition comprising the same, preferably in combination with a stimulus responsive polymer. Consequently, methods to obtain the said compositions, along with therapeutic applications in improvement in thickness and receptivity of the endometrial lining are provided.

Apparatus and methods for concentrating platelet-rich plasma is described herein. One variation may generally comprise a tube having a length and defining a channel within and one or more ports located at a proximal end of the tube and in fluid communication with the channel. A plunger may slidably translatable within the channel while forming a seal against an inner surface of the channel and a float may have a pre-selected density and defining a concave interface surface, wherein the float is slidably contained within the channel such that the concave interface surface is in apposition to the one or more ports.

A method for filling containers with fresh blood components, comprising the following steps of: a) providing a piece of equipment (1) defining a transit channel (3) having at least a first gap (3a) associated with a bag (5) or the like containing a fresh blood component, at least a second gap (3b) connected to a plurality of containers (6) to be filled, and at least a third gap (3c) connected to pumping means (12); b) isolating the first gap (3a) from the second gap (3b) and placing the latter in communication with the third gap (3c); c) suctioning the air contained in the containers (6) to be filled by means of the pumping means (12) so as to define a vacuum inside them; d) placing the first gap (3a) in communication with the second gap (3b), the containers (6) suctioning the contents of the bag (5) by effect of the vacuum defined inside them; and where the channel (3) has at least a fourth gap (3d) associated with an empty syringe (4) or the like, and by the fact that it comprises, after step d), the following steps of: e) isolating the first gap (3a) from the second gap (3b) and placing the first gap itself in communication with the fourth gap (3d); f) taking at least a part of the contents of the bag (5) by means of the syringe (4); g) isolating the fourth gap (3d) from the first gap (3a) and placing the fourth gap itself in communication with the second gap (3b); h) sending the contents taken with the syringe (4) into the containers (6).

The present disclosure provides apparatuses and systems for delivering a measureable absorbed-dose of a gaseous activating agent to a fluid including a biological liquid and/or cells. The apparatuses or systems include a gas-fluid contact device configured to controllably rotate or oscillate a control member having an interior surface in contact with the fluid and a control system configured to control rotation or oscillation of the contact member by the gas-fluid contact device. In some embodiments, the control system is further configured to control absorption of the gaseous activating agent by the fluid. The present disclosure also provides methods of treating a fluid including a biological liquid or cells with a gaseous activating agent to controllably activate the fluid.

Provided herein are methods for the isolation of platelets, for example, isolation of platelets from umbilical cord blood. In certain embodiments, presented herein are methods for preparation of platelet rich plasma. In one aspect, provided herein are methods for isolation of platelets from blood. In certain embodiments, presented herein are methods for isolation of platelets from cord blood, e.g., human cord blood. The isolated platelets can be used for a variety of applications, including, for example, methods of wound healing, organ repair and/or regeneration, and/or tissue repair and/or regeneration, in either autologous or allogenic settings.

Method and Apparatus for predicting the volume of a component separated from a composite fluid by predicting the volume of the composite fluid from sensed pressure and predicting the volume of other separated components from sensed movement of the other components to collection bags.

A system and methodology for the preservation of red blood cells is described in which red blood cells are oxygen or oxygen and carbon dioxide depleted, treated and are stored in an anaerobic environment to optimize preparation for transfusion. More particularly, a system and method for extended storage of red blood cells from collection to transfusion that optimizes red blood cells prior to transfusion is described.