Provided is a centrifugation method for extracting a target component from an object including: providing a centrifugal container including a first space in an upper portion, a second space in a lower portion, a nozzle connecting the first space and the second space, a second plunger movably received in the second space, and a nozzle blocking member to penetrate the center of the second plunger in a longitudinal direction and to linearly move on the second plunger to selectively open/close a lower end of the nozzle; storing the object; interlayer-separating the object by primarily centrifuging the object; passing only the target component in the object; blocking the lower end of the nozzle by means of the nozzle blocking member; resuspending components remaining in the first space and the nozzle while the nozzle is blocked; and extracting a target component resuspended in the first space.

A syringe holder for holding a syringe in a centrifuge, the syringe having a plunger-end and a nipple-end, the syringe holder holding the syringe nipple-end upwardly, comprising: a cup having a cup seat, a wall, a bore, a mouth, and a support, the support supporting the cup in the centrifuge; a lower support having a upper surface and a lower surface, the lower support sized to slide into the cup and the lower support supporting the plunger-end of the syringe; an upper support having lower section and an upper section with a lip, and having a channel therethrough, the lip having a outer diameter larger than the inner diameter of the mouth of the cup, the channel therethrough co-axial with the lower support and the bore of the cup, the lower section having an outer diameter that is less than the inner diameter of the bore of the of the cup and sized to permit the upper support to slide at least partially into the cup and the lip to contact the mouth of the cup and support the upper support in the cup; whereby the upper support and the lower support support the syringe with the needle-end upwardly in the cup during centrifuging.

A dual chambered syringe includes: an inner barrel defining a first inner chamber, the inner barrel having an apertured stopper at its distal end, the inner barrel being open at its proximal end; a shaft adapted to fit within the inner barrel, the shaft having a distal end which is engageable with the aperture of the stopper; a device for controlling engaging and disengaging of the distal end of the shaft with the aperture of the stopper; an outer barrel concentric with the inner barrel defining a second inner chamber, the outer barrel having a distal end for receiving and dispensing fluids and a proximal end into which the distal end of the inner barrel is insertable into the second inner chamber; the apertured stopper engages the second inner chamber of the outer barrel and selectively prevents or permits the passage of fluids between the outer barrel second chamber and the inner barrel first chamber; the inner barrel having an engageable surface on its outside surface; and, the outer barrel having operatively associated therewith an engaging device for selective engagement and disengagement with the engageable surface on the inner barrel.

A method for collecting plasma includes determining the weight and hematocrit of a donor, and inserting a venous-access device into the donor. The method then withdraws blood from the donor through a draw line connected to a blood component separation device, and introduces anticoagulant into the withdrawn blood. The blood component separation device separates the blood into a plasma component and a second blood component, and the plasma component is collected from the blood component separation device and into a plasma collection container. The method may then calculate (1) a percentage of anticoagulant in the collected plasma component, and (2) a volume of pure plasma collected within the plasma collection container. The volume of pure plasma may be based, at least in part, on the calculated percentage of anticoagulant. The method may continue until a target volume of pure plasma is collected within the plasma collection container.

Apparatus and methods for concentrating platelet-rich plasma is described herein. One variation may generally comprise a tube having a length and defining a channel within and one or more ports located at a proximal end of the tube and in fluid communication with the channel. A plunger may slidably translatable within the channel while forming a seal against an inner surface of the channel and a float may have a pre-selected density and defining a concave interface surface, wherein the float is slidably contained within the channel such that the concave interface surface is in apposition to the one or more ports.

Disclosed is a multiple bag system for fractionating blood, the system including a fluid collecting bag including at least one outlet port; at least first and second sampling bags, each including at least one inlet port and at least one outlet port; and a fluid transfer unit to transfer fluid from the collecting bag to the sampling bags. The fluid transfer unit includes an acoustic sorter. Also disclosed is a method for fractionating blood into blood products.

A method for automated processing of a blood product, the method comprising providing a reusable separation apparatus controlled by a microprocessing unit, said apparatus configurable with settings and configured to associate with a disposable circuit comprising a separator and in communication with a source blood product having a first concentration and first volume. The apparatus and disposable circuit are configured to flow the source blood product into an inlet of the separator and separate supernatant of the source blood product from a first outlet of the separator into a filtrate container. The apparatus and disposable circuit are also configured to separate platelets and remaining supernatant from a second outlet of the separator into a retentate container, wherein the platelets and remaining supernatant in the retentate container have a second concentration greater than the first concentration and second volume less than the first volume.

The invention relates to an apparatus for receiving a body fluid, wherein the apparatus comprises a first chamber with a displaceable wall and a second chamber of a constant effective size, said second chamber being movable with respect to the displaceable wall, wherein the apparatus is designed to connect the first chamber and the second chamber during a longitudinally axial movement of the second chamber with respect to the displaceable wall and to transfer body fluid from the first chamber to the second chamber during a movement of the second chamber together with the displaceable wall.

In the presented invention, by using two connected polymer bags and the connection to the drawing blood, the first separation in the first bag and transmission of the blood components; such as RPP into the second bag and re-injecting the remaining blood to the considered person are possible.

The present invention relates to a system for separating biological fluids ink components that makes use of a hollow centrifugal processing chamber of variable volume. The system is a functionally closed centrifugation chamber that extracts sub-components of a biological fluid according to their density and size, such as platelets, plasma or red cells from whole blood.