A blood bag system (10) includes a blood bag (14) to which centrifugal force is provided in a state where whole blood or a blood component is stored, and a first tube (22) that circulates a fluid centrifugally separated from the blood bag (14). The first tube (22) includes an extending portion (112) extending in an approximately perpendicular direction to a centrifugal direction into which the centrifugal force is applied. Further, a branch tube (114) that can store blood existing in the extending portion (112) is provided at a side of the centrifugal direction of the extending portion (112).

The present invention provides a second use of mitochondria, which can cure a tendon injury-related disease and prevent a disease caused by a tendon injury. Specifically, the mitochondria disclosed in the present invention have the effect of repairing injured tendon cells and accelerating the healing of the tendon cells. Therefore, by administering a predetermined amount of mitochondria or a composition containing a predetermined amount of mitochondria to a part with a tendon injury, wound healing of the part with the tendon injury can be promoted, thus achieving the effect of repairing the injured tendon and further preventing a joint disease caused by the tendon injury or inflammation.

Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.

A process for the sequential processing of opaque and transparent biological fluids such as whole blood, apheresis blood, bone marrow blood, umbilical cord blood, buffy coat or cultured cells by processing steps in a hollow cylindrical centrifugal processing chamber (300) which is part of a disposable set. At least three different procedures selected from washing, incubation, transduction, separation, density gradient separation, dilution and volume adjustment are each carried out once or repeated a number of times according to a given processing profile in the processing chamber. Each procedure involves an input into the processing chamber, an operation in the processing chamber and an output from the processing chamber by displacement of a piston (310). The at least three different procedures are sequentially chained one after the other to constitute an overall sequential operation in the processing chamber and its disposable set. A first application is incubation for binding magnetic beads with human blood cells or stem cells. A second application is transduction by which foreign genetic material is inserted into human blood cells or stem cells by a virus. A third application is reconditioning biological fluids to achieve reproducible concentration and volumes of blood cells or stem cells.

A fluid processing system for controlling fluid flow comprises a cassette having a defined passageway on a first side. The first side includes flexible sheeting disposed over the passageway. The system comprises a durable processing device configured to engage the first side of the cassette, the durable processing device comprising a valve actuator configured to engage the flexible sheeting at a valve location along the passageway. The system comprises a first pump configured to draw fluid away from the valve location along the passageway. The first pump is disposed downstream of the valve location. The system comprises a second pump configured to pump fluid towards the valve location along the defined passageway. The second pump is disposed upstream of the valve location. The first and second pumps are configured to operate in concert and configured to provide pressure to prevent collapsing of the flexible sheeting against the passageway during operation.

A medical device for the separation of a concentrate enriched with cells from a biological fluid, in particular a concentrate of stromal or stem cells from medullar aspirate or venous blood, comprises: a treatment container for receiving and treating the biological fluid for the purposes of its separation into a number of fractions; a first collection container, for receiving a first fraction of the fluid, in particular a fraction poor in said cells; a second collection container, for receiving a second concentrated fraction of the fluid enriched with said cells; a deviator valve having a first way connected or prearranged for connection to the treatment container, a second way connected or prearranged for connection to the first collection container, and a third way connected or prearranged for connection to the second collection container; a first line for connection of the treatment container to the first way of the deviator valve, a second line for connection of the first collection container to the second way of the deviator valve, and a third line for connection of the second collection container with the third way of the deviator valve.

The treatment container is a syringe container with a plunger having a plunger stem associated in a releasable way to a corresponding plunger head, and the first connection line comprises a first transparent tube, in particular a flexible transparent tube, and at least one of the second and third connection lines preferably comprises a second flexible tube. At least one connection line, or each connection line, further comprises: at least one respective hydraulic connector, for separable connection of a corresponding tube to the corresponding container and/or to the corresponding way of the deviator valve; and at least one automatic-closing or one-way valve.

A system and associated method for concentrating and separating components of different densities from fluid containing cells using a centrifuge includes a container defining a cavity for receiving the fluid. The container has a top, a sidewall extending from the top, and a bottom disposed opposite the top and in sealing engagement with the sidewall. An insert is slidably disposed in the cavity of the container and defines a lumen through the insert. The lumen, which includes a hole and a funnel-shaped upper portion in fluid communication with the hole, forms an open fluid path between opposite ends of the insert. The insert has a density such that upon centrifugation a selected component of the fluid resides within the lumen. A container port is disposed in the top of the container to transfer the fluid into the container and to withdraw a fluid component other than the selected component from the container. The system includes a manifold that includes a manifold port, a vent to vent the container, and a connector to couple to the container port. A cannula is receivable in the manifold port and extendable through the container port into the container and into the lumen of the insert to withdraw the selected component from the lumen.

A blood component separator (1) includes a blood storage vessel (20) that includes a first storage part (21) and a second storage part (22), a slider (30) movable from the first storage part to the second storage part, and a flow path (40f) for communicating an inside and an outside of the storage vessel. When the slider is in the first storage part, the first storage part and the second storage part are in communication with each other. When the slider is inserted into the second storage part, a liquid-tight seal is formed between the slider and the inner peripheral surface of the second storage part and the communication between the first storage part and the second storage part is blocked by the slider. The slider is movable in the second storage part while maintaining the liquid-tight seal between the slider and the inner peripheral surface of the second storage part. As the slider enters the second storage part, the blood component in the second storage part is pushed out of the storage vessel through the flow path.

The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems, devices, and methods of obtaining, utilizing, and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.

A system for blood cell separation, comprising:

a separation chamber (10) comprising an inlet port for blood (12), an outlet port for plasma (14) and at least an outlet port for cellular blood components (16) for the separation of whole blood;

a blood pump (20) for pumping whole blood into the inlet port for blood (12);

a plasma pump (22) for pumping plasma and/or target cells from the outlet port for plasma (14) out of the separation chamber (10);

a red blood cell tube (30) comprising a first end (32) and a second end (34), wherein the first end (32) of the red blood cell tube (30) is connected to the outlet port for cellular blood components (16) for allowing red blood cells to leave the separation chamber (10); and

a drip chamber (40) comprising a reservoir (42) and an inlet (46), wherein the second end (34) of the red blood cell tube (30) is connected to the inlet (46), wherein the second end (34) of the red blood cell tube (30) extends into the volume of the reservoir (42) for pressure equalization during pumping from the outlet port for plasma (14).