The embodiments of the present disclosure provide a cell culture composition and a use thereof, and the cell culture composition includes a culture medium and mitochondria. The cell culture composition including mitochondria can promote cell growth and improve the function of the damaged or aged stem cells, thereby improving overall cell growth.

The embodiments of the present disclosure provide a composition including mitochondria. By providing the mitochondria to cartilage, the composition can improve the mitochondrial function of the chondrocytes in the cartilage so as to increase the repair ability of the chondrocytes and repair the cartilage damage caused by aging of the chondrocytes, thereby achieving the purpose of treating osteoarthritis.

Apparatus and methods for processing blood are disclosed in which one variation generally comprises a tube defining a channel and an access tube extending into the channel. An open cell matrix configured to entrap red blood cells may be positioned within at least a portion of the channel. Another variation generally comprises a cylindrical tube and a plunger slidably positioned within the channel. The plunger also has a funnel positioned upon the plunger and is movable therewith. Both the plunger and funnel define a fluid channel through and in communication with the cylindrical tube.

A container to be used for preparing a multi-layered blood product by centrifugation comprises a substantially cylindrical body (2) with a closed bottom (3) and a top (4) having a filling opening as well as a filter device (15), which is slidable inside the container (1). The filing opening is closed by means of a penetrable membrane (13), and the filter device (15) comprises a substantially planar mesh (16) and a supporting buoyancy body (18). In addition to this, there are means (35, 36, 39, 40, 42), for releasable fixation of the filter device (15) adjacent to the bottom (3) of the container (1).

The present disclosure relates to methods and devices for the separation of blood components including separation by rapid sedimentation, including in an automated fashion.

Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.

A flow path device of a biological component bag system is equipped with a device main body. The device main body includes a first sheet and a second sheet, and flow paths are formed between the first sheet and the second sheet. The flow paths include a first flow path, a second flow path, a filter accommodating chamber, and a third flow path. In the device main body, flow path sealed portions that join the first sheet and the second sheet to each other in a liquid-tight manner are disposed along the flow paths.

A vented dual port centrifuge tube includes a tubular receptacle having upper and lower ends. A first common inlet and outlet port is formed in the upper end and a second common inlet and outlet port is formed in the lower end. A piston is sealably and slidably mounted within a chamber of the receptacle. A vent is supported in the upper end of the receptacle and a flexible vent pipe attached to the piston is communicably interconnected between the vent and a lower region of the chamber between the piston and the lower end of the receptacle. Air pressure in the lower region of the receptacle is equalized or neutralized as biological fluids and separated constituent components are introduced into and aspirated from the receptacle through the common inlet ports.

Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.

Provided is a centrifuge cassette assembly (800) for separating a fluid and related method of manufacture. The cassette assembly includes a first chamber, a second chamber (308), a fluidic channel (808) creating a fluid connection between the first chamber and the second chamber, at least one molded insertion valve (600, 700) configured to control the flow of fluid in the fluidic channel and a heating element (802) for actuating the at least one molded valve. Further provided are Normally Open Valves (NOVs) (700) and Normally Closed Valves (NCVs) (600) which are capable of insertion into, and which control the fluid flow of, the centrifuge cassette assembly.