An autotransfusion system for separating fluid constituents includes a centrifuge housing and a rotatable driving member mounted within the centrifuge housing. The rotatable driving member is configured to receive therein and rotationally engage any one of a plurality of centrifuge bowls with different heights. In some embodiments, the centrifuge bowl is integrated with a fluid line organizer to provide for easy and efficient organization of a plurality of different fluid lines incorporated into the autotransfusion system. In some embodiments, the centrifuge bowl and fluid line organizer are easily and efficiently coupled to the centrifuge housing for autotransfusion processing.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

A tubing set for use in a blood processing apparatus comprises a measurement device (8) having at least one chamber element (80, 81) for measuring a haematocrit value of a blood fluid, wherein the at least one chamber element (80, 81) extends along a longitudinal axis (L) and comprises a circumferential wall (804, 814) extending about the longitudinal axis (L) and encompassing a flow chamber (802, 812), the at last one chamber element (80, 81) further comprising an inlet port (800, 810) for allowing a flow of a blood fluid into the flow chamber (802, 812) and an outlet port (801, 811) for allowing a flow of a blood fluid out of the flow chamber (802, 812). The tubing set furthermore comprises an inlet-side tube section (21, 31) connected to the inlet port (800, 810) and an outlet-side tube section (22, 30) connected to the outlet port (801, 811). Herein, the inlet port (800, 810) and the outlet port (801, 811) are arranged on the circumferential wall (804, 814) and are displaced with respect to each other along the longitudinal axis (L). In this way a tubing set comprising a measurement device is provided which in an easy and reliable manner allows for the measuring of a haematocrit value of a blood fluid.

In a method for producing a cell concentrate using a cell suspension treatment system including a storage container of a cell suspension, which has a solution inlet port, a circulation outlet port, and a circulation inlet port, a cell suspension treatment device for concentrating the cell suspension by separating liquid from the cell suspension by filtration, the device including a container having a cell suspension introduction port, a cell suspension lead-out port, and a filtrate outlet, which is filled with a hollow fiber separation membrane, a circulation circuit for concentrating the cell suspension while circulating the cell suspension between the storage container and the cell suspension treatment device, a collection container of a cell concentrate obtained by concentration, a collection path for feeding the cell concentrate to the collection container, an injection path for injecting a solution into the solution inlet port of the storage container, and a detecting unit.

A system for concentrating cells, wherein a syringe comprises a lumen and an axial end comprising a port and a radial end closed to liquid flow. A plunger divides the axial and radial ends, and the syringe is configured to hold a cellular suspension. A filter disposed at the radial end is configured to maintain sterility of the syringe. A cap comprises a vent disposed at the radial end. The plunger is configured to be actuated towards the axial end by air pressure being applied into the radial end and the plunger is configured to be actuated towards the radial end by a vacuum being applied into the radial end.

Systems and methods for performing online extracorporeal photopheresis of mononuclear cells are disclosed. Whole blood is removed from a patient and introduced through a processing set into a separation chamber to separate the desired cell population from the blood. The separated cell population is processed through the set which is associated with a treatment chamber where the cells are treated. Once treated, the cells are returned to the patient. The processing set remains connected to the patient during the entire ECP treatment procedure and provides an online, sterile closed pathway between the separation chamber and the treatment chamber.

Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating undesirable particles bound to capture particles from formed elements of whole blood. After introducing the capture particles to whole blood containing undesirable particles, the whole blood and capture particles are flowed through a microfluidic separation channel. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements and undesirable particles bound to capture particles to specific aggregation axes. After aggregating the particles, the formed elements exit the separation channel through a first outlet and are returned to the patient. The undesirable particles, bound to the capture particles, exit through a second outlet and can be discarded to saved for later study.

A process for the sequential processing of opaque and transparent biological fluids such as whole blood, apheresis blood, bone marrow blood, umbilical cord blood, buffy coat or cultured cells by processing steps in a hollow cylindrical centrifugal processing chamber (300) which is part of a disposable set. At least three different procedures selected from washing, incubation, transduction, separation, density gradient separation, dilution and volume adjustment are each carried out once or repeated a number of times according to a given processing profile in the processing chamber. Each procedure involves an input into the processing chamber, an operation in the processing chamber and an output from the processing chamber by displacement of a piston (310). The at least three different procedures are sequentially chained one after the other to constitute an overall sequential operation in the processing chamber and its disposable set. A first application is incubation for binding magnetic beads with human blood cells or stem cells. A second application is transduction by which foreign genetic material is inserted into human blood cells or stem cells by a virus. A third application is reconditioning biological fluids to achieve reproducible concentration and volumes of blood cells or stem cells.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

An autotransfusion system for separating fluid constituents includes a centrifuge housing and a rotatable driving member mounted within the centrifuge housing. The rotatable driving member is configured to receive therein and rotationally engage any one of a plurality of centrifuge bowls with different heights. In some embodiments, the centrifuge bowl is integrated with a fluid line organizer to provide for easy and efficient organization of a plurality of different fluid lines incorporated into the autotransfusion system. In some embodiments, the centrifuge bowl and fluid line organizer are easily and efficiently coupled to the centrifuge housing for autotransfusion processing. After autotransfusion processing, the centrifuge bowl and fluid line organizer are easily and efficiently decoupled from the centrifuge housing and discarded.