A method of decreasing hypercoagulability and/or increasing plasma clotting time comprising removing red cell-derived particles (RCDP) from plasma.

A modular cassette and method for separating a composite fluid into at least two component parts thereof during centrifugation is provided. The modular cassette includes a fluid inlet portion, at least one fluid separation portion, at least one media chamber in fluid communication with the fluid separation portion, a fluid collection portion, at least one fluidic channel configured to form a fluid communication between at least two components of the cassette, at least one wax valve including undulating flow channel portions configured to close at least one of the fluidic channels, and at least one heating element configured to actuate the at least one wax valve.

A modular cassette and method for separating a composite fluid into at least two component parts thereof during centrifugation is provided. The modular cassette includes a fluid inlet portion, at least one fluid separation portion, at least one media chamber in fluid communication with the fluid separation portion, a fluid collection portion, at least one fluidic channel configured to form a fluid communication between at least two components of the cassette, at least one wax valve including undulating flow channel portions configured to close at least one of the fluidic channels, and at least one heating element configured to actuate the at least one wax valve.

A method and system is provided that measures plasma protein levels of whole blood while a plasma donor is connected to an apheresis machine. A refractometer associated with the apheresis machine is capable of receiving a portion of a disposable tubing set including an integrated cuvette and prism. The integrated cuvette of the disposable tubing set can be inserted into a receiving space of the refractometer associated with the apheresis machine such that the light source and the sensor are oriented relative to the prism and a sensing surface of the integrated cuvette in a precise alignment. Calibration of the refractometer is made using anticoagulant pumped through the disposable tubing set including the integrated cuvette and prism. Based on a light intensity associated with this calibration, whole blood is then measured to determine plasma protein levels and donor eligibility.

A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma/bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow/platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate/platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate/platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma/bone marrow concentrate fractions.

A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma/bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow/platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate/platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate/platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma/bone marrow concentrate fractions.

A dual chambered syringe includes: an inner barrel defining a first inner chamber, the inner barrel having an apertured stopper at its distal end, the inner barrel being open at its proximal end; a shaft adapted to fit within the inner barrel, the shaft having a distal end which is engageable with the aperture of the stopper; a device for controlling engaging and disengaging of the distal end of the shaft with the aperture of the stopper; an outer barrel concentric with the inner barrel defining a second inner chamber, the outer barrel having a distal end for receiving and dispensing fluids and a proximal end into which the distal end of the inner barrel is insertable into the second inner chamber; the apertured stopper engages the second inner chamber of the outer barrel and selectively prevents or permits the passage of fluids between the outer barrel second chamber and the inner barrel first chamber; the inner barrel having an engageable surface on its outside surface; and, the outer barrel having operatively associated therewith an engaging device for selective engagement and disengagement with the engageable surface on the inner barrel.

A dual chambered syringe includes: an inner barrel defining a first inner chamber, the inner barrel having an apertured stopper at its distal end, the inner barrel being open at its proximal end; a shaft adapted to fit within the inner barrel, the shaft having a distal end which is engageable with the aperture of the stopper; a device for controlling engaging and disengaging of the distal end of the shaft with the aperture of the stopper; an outer barrel concentric with the inner barrel defining a second inner chamber, the outer barrel having a distal end for receiving and dispensing fluids and a proximal end into which the distal end of the inner barrel is insertable into the second inner chamber; the apertured stopper engages the second inner chamber of the outer barrel and selectively prevents or permits the passage of fluids between the outer barrel second chamber and the inner barrel first chamber; the inner barrel having an engageable surface on its outside surface; and, the outer barrel having operatively associated therewith an engaging device for selective engagement and disengagement with the engageable surface on the inner barrel.

A blood component separation device for separating a plurality of blood components from blood sampled from a blood donor, and collecting platelets, includes: an operation unit that calculates a predicted platelet recovery rate from a hematocrit value of the blood and a platelet concentration of the blood, and calculates a recommended processing amount of the blood recommended for collecting a target number of units of platelets on the basis of the calculated predicted platelet recovery rate, wherein the operating unit sets the predicted platelet recovery rate calculated from any the hematocrit value and any the platelet concentration to be smaller by a predetermined value a when the blood donor is female than that when the blood donor is male.

Apparatus and methods for concentrating platelet-rich plasma is described herein. One variation may generally comprise a tube having a length and defining a channel within and one or more ports located at a proximal end of the tube and in fluid communication with the channel. A plunger may slidably translatable within the channel while forming a seal against an inner surface of the channel and a float may have a pre-selected density and defining a concave interface surface, wherein the float is slidably contained within the channel such that the concave interface surface is in apposition to the one or more ports.