An adipose tissue (AT) transfer system includes, on the aspiration side, an aspiration cannula, an aspiration pump, a container, and flexible tubing connecting the aspiration cannula to the container. On the reinjection side, the system includes a reinjection cannula, flexible tubing connecting the inlet of the reinjection cannula to the container, and a reinjection pump imposing positive-displacement pumping action on the flexible tubing and causing movement of AT in a pulsed mode. The aspiration pump operates to continually supply harvested AT to the second flexible tubing while the reinjection pumps causes continuous or pulsed deposition of the AT at injection site. To ensure that internal pressure and/or flow of the AT through a channel of delivery of the AT to the reinjection site does not exceed a predetermined value, the system contains an external pressure sensor configured to measure such internal pressure in absence of a part that is in direct contact with the AT.

An apparatus for processing biological material includes a supporting structure, provided with a seat and with a rotation device supplied with constraining means, and a container for biological material, provided with a shaped body, with a base, shaped to match the seat of the supporting structure, with at least one valve (24a) for extracting the biological material, and with movable cutting means equipped with coupling means for coupling to the constraining means of the rotation device, in such a way as to acquire a relative motion relative to the container.

A deagglomerator for use in resizing masses of cells. The deagglomerator may include a plurality of apertures defined by a plurality of front and back edges. The masses of cells may be passed through the plurality of apertures from the front to the back, and from the back to the front, repeatedly. The deagglomerator may also include a plurality of blades that may aid in the deagglomeration of the cell masses. The deagglomerator may be configured between two syringes so that the tissue may be passed back and forth from the first syringe through the device to the second syringe, and then back again from the second syringe through the device and to the first syringe. In this way, the masses of cells may be properly deagglomerated.

A method for processing biological material containing stringy tissue in a container having a tissue collector disposed in a tissue retention volume on one side of an internal filter includes washing biological material contained in the tissue retention volume with wash liquid to the tissue retention volume and allowing the wash liquid and rotating the tissue collector disposed in the tissue retention volume relative to the container in a first direction of rotation about an axis of rotation to sweep the teeth positioned on the tissue collector through the biological material and to collect stringy material on the tissue collector.

An adipose tissue (AT) transfer system includes, on the aspiration side, an aspiration cannula, an aspiration pump, a container, and flexible tubing connecting the aspiration cannula to the container. On the reinjection side, the system includes a reinjection cannula, flexible tubing connecting the inlet of the reinjection cannula to the container, and a reinjection pump imposing positive-displacement pumping action on the flexible tubing and causing movement of AT in a continuous or pulsed mode. The aspiration pump operates to continually supply harvested AT to the second flexible tubing while the reinjection pumps causes continuous or pulsed deposition of the AT at the injection site. To ensure that internal pressure and/or flow of the AT through a channel of delivery of the AT to the reinjection site does not exceed a predetermined value, the system contains an external pressure sensor configured to measure such internal pressure in absence of a part that is in direct contact with the AT.

A tissue processing apparatus, a filter and a method for processing tissue therefrom #! The problem to be solved is to provide a portable apparatus for processing harvested fat to form a good quality graft that does not clog syringes, and the problem is solved by providing an apparatus as in the present invention with a filter that is inclined to the base of the apparatus and comprises of plurality of elongated protrusion that are structured at an angle on the surface of the filter and blocks the fibres when the harvested fat moves on the inclined surface of the filter, thereby filtering the harvested fat and avoiding clogging of syringes.

Preparation of a product comprising stromal vascular fraction cells includes washing human biological material comprising adipose in a container apparatus having an internal filter, which divides an internal containment volume of the container apparatus into a tissue retention volume on one side of the filter and a filtrate volume on an opposite side of the filter, and a mixing device with at least one rotatable mixing member disposed in the tissue retention volume. The washing includes operation of the mixing device to rotate the mixing member through the human biological material within the tissue retention volume, and the washing is followed by digesting washed material within the internal containment volume with added enzyme, centrifuging of the container apparatus to prepare a centrifuged pellet in the filtrate volume, selectively removing material of the pellet and preparing a product with a mixture of stromal vascular fraction cells of removed pellet material and an aqueous suspension liquid.

A closed system for harvesting fat through liposuction, concentrating the aspirate so obtained, and then re-injecting the concentrated fat into a patient comprises as its main components a low pressure cannula having between about 7 to 12 side holes of about 1-2 mm by 2.0 to 4.0 mm, a spring loaded syringe holder with a constant force or coiled ribbon spring to apply a substantially constant pressure over the full excursion of the plunger, and a preferably flexible collection bag which is also preferably graduated, cylindrical over most of its body and funnel shaped at its bottom, all of which are connected through flexible tubings to a multi-port valve. The multi-port valve has two flutter/duck bill valves which restrict the fluid flow to a one way direction which effectively allows the syringe to be used to pump fat out of a patient and into a collection bag in a continuous manner. After the bags are centrifuged to concentrate the fat, the excess fluids are separated and the valve is re-connected to permit the syringe pump to reverse fluid flow to graft the concentrated fat back into the patient.

Methods of collecting fat tissue samples in tissue collection syringes mounted in an in-line fat sampling device connected to a hand-held power-assisted tissue aspiration instrument.

Embodiments of the present invention disclose micro-lipo needle device and methods of making and using the same.