An adipose tissue (AT) transfer system includes, on the aspiration side, an aspiration cannula, an aspiration pump, a container, and flexible tubing connecting the aspiration cannula to the container. On the reinjection side, the system includes a reinjection cannula, flexible tubing connecting the inlet of the reinjection cannula to the container, and a reinjection pump imposing positive-displacement pumping action on the flexible tubing and causing movement of AT in a continuous or pulsed mode. The aspiration pump operates to continually supply harvested AT to the second flexible tubing while the reinjection pumps causes continuous or pulsed deposition of the AT at the injection site. To ensure that internal pressure and/or flow of the AT through a channel of delivery of the AT to the reinjection site does not exceed a predetermined value, the system contains an external pressure sensor configured to measure such internal pressure in absence of a part that is in direct contact with the AT.

The invention is a process and system, consisting of a reinjection pump, connecting tubing, a reinjection cannula and a recipient site, with fluid management features including a peristaltic pump head and controls for pressure limits, flow rates and flow distribution wherein the process of controlling the reinjected fat and fluid is unique because of the control of flow rates, pressures, matching cannula hole sizes, and maintaining a closed continuous system where the harvesting and reinjection is done all together with a completely closed system. The process utilizes a method and control system to manage pressure levels during reinjection procedures of viable soft tissue. Although this case specifically relates to its use in the reinjection of adipose fat and other tissue back into the body during liposuction procedures, it can be applied to any medical procedure of introducing or re-introducing materials into the body.

A closed system for harvesting fat through liposuction, concentrating the aspirate so obtained, and then re-injecting the concentrated fat into a patient comprises as its main components a low pressure cannula having between about 7 to 12 side holes of about 1-2 mm by 2.0 to 4.0 mm, a spring loaded syringe holder with a constant force or coiled ribbon spring to apply a substantially constant pressure over the full excursion of the plunger, and a preferably flexible collection bag which is also preferably graduated, cylindrical over most of its body and funnel shaped at its bottom, all of which are connected through flexible tubings to a multi-port valve. The multi-port valve has two flutter/duck bill valves which restrict the fluid flow to a one way direction which effectively allows the syringe to be used to pump fat out of a patient and into a collection bag in a continuous manner. After the bags are centrifuged to concentrate the fat, the excess fluids are separated and the valve is re-connected to permit the syringe pump to reverse fluid flow to graft the concentrated fat back into the patient.

Methods of collecting fat tissue samples in tissue collection syringes mounted in an in-line fat sampling device connected to a hand-held power-assisted tissue aspiration instrument.

The invention is a method and control system to manage pressure levels during reinjection procedures of viable soft tissue. Although this case specifically relates to its use in the reinjection of adipose fat and other tissue back into the body during harvesting and reinjection procedures, it can be applied to any medical procedure of introducing or re-introducing materials into the body.

A process and system of compatible cannulas with features that are linked and intentionally related including the Interior Cross Sectional Area (302) of the cannulas, the area of the opening(s) of the cannulas and the width of the opening(s) of the cannulas (303). When used in a method where the appropriate harvesting cannula (100) is selected for use with a desired reinjection cannula (100), the cannula kit (FIG. 8) provides increased benefit for maintaining live tissue viability, and minimizes the time of the surgical procedure. The cannula (100) as a kit or set (FIG. 8) is used for harvesting and for reinjection, and serves in extracting, processing and transferring adipose tissue.

Methods and devices are disclosed for processing stromal precursor cells (i.e., cells which can differentiate into connective tissue cells, such as in muscles, ligaments, or tendons) which can be obtained from fatty tissue extracts obtained via liposuction. Normal processing of a liposuction extract involves centrifugation, to concentrate the stromal cells into a semi-concentrated form called “spun fat”. That “spun fat” can then be treated by mechanical processing (such as pressure-driven extrusion through 0.5 mm holes) under conditions which can gently pry the stromal cells away from extra-cellular collagen fibers and other debris in the “spun fat”. The extruded mixture is then centrifuged again, to separate a highly-enriched population of stromal cells which is suited for injection back into the patient (along with platelet cells, if desired, to further promote tissue repair or regeneration).

Disclosed is a vessel for recovering adipose tissues including a body sealed by a lid connectable to a first pipe with a suction cannula, and a second pipe with a suction pump. A removable adapter connects a lid including two orifices, each closed by a stopper that can be pierced or is pre-pierced and self-sealing, and a vent opening with a filter and removable plug. The connection adapter includes two rigid piercing tubes projecting from under the adapter, each piercing tube in continuous fluid relationship with a corresponding nipple projecting from the adapter, each nipple connectable to a corresponding pipe. The two sealed orifices of the lid and the adapter and piercing tubes are arranged so, when the connection adapter is applied to the lid, each piercing tube pierces a corresponding stopper and brings each nipple into correspondence, in a sealed manner, with the internal volume of the vessel.

A triple-chambered container includes: a main container body with a first chamber which has an opening at its distal end; a main barrel formed and movable longitudinally within the first chamber, the main barrel defining therein a second chamber for receiving fluids, the main barrel further having an apertured stopper at its distal end; a second barrel formed within the main barrel, the second barrel defining a third chamber for receiving fluids, the second barrel being movable longitudinally within and with respect to the main barrel, the second barrel having a distal end which is engageable and disengageable with the apertured stopper; a shaft adapted to fit within the second barrel, the shaft being movable longitudinally within and with respect to the second barrel, the shaft having a distal end which is engageable and disengageable with an aperture in the second barrel; a device for controlling engaging and disengaging of the distal end of the shaft with the aperture of the second barrel. The first chamber, the second chamber and the third chamber may be selectively moved to receive and discharge fluids with respect to one another.

A method for skin ligament injection incorporates the benefits of regenerative medicine to provide more natural and longer lasting results in the revitalization of a patient's face. The method includes a first phase in which through the use of aspiration tubes with reduced diameter, small aspiration holes and controlled aspiration pressure with aspiration in a superficial plane of the skin, a user collects tissue rich in stein cells with waste of fibrotic tissue. A second phase includes the sterile manipulation and washing of fat to collect only the tissue containing the stein cells arranged around vascular structures and growth factors collected inside organelles called exosomes. Lastly, a third and final phase includes injecting the obtained product into the skin of a patient.