The present disclosure provides a composition comprising, (i) protein at a protein content of at least about 16% by dry weight, wherein at least about 50% by dry weight of the protein has an amino acid sequence encoded by one or more fungal genomes, and (ii) one or more materials selected from the group consisting of a flour, an oil, a flavoring agent, and a nutritional supplement, wherein the composition has a water content that is less than or equal to about 30% water by weight.

The present invention provides edible non-animal dairy substitute products comprising fibrous mycelium as protein component. The substitute products offer an even higher nutritional value compared to dairy products in terms of its high prebiotic insoluble fiber content critical for gut health. Such high fibrous ingredient is not known to be contained with such properties in dairy products. The edible non-animal dairy substitute product of the present invention is selected from products substituting milk, yoghurt, fresh cheese, whey cheese, cream cheese, medium-hard cheese, hard-cheese, and soft-mould cheese. The present invention is also concerned with methods of producing the edible non-animal dairy substitute products.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Disclosed herein is a method for separating a protein from a microorganism comprising a cell wall. Further compositions comprising a first fraction, a second fraction or both, derived from a microorganism comprising a cell wall and comprising a protein content between 10% and 90% by weight of the fraction, are also disclosed.

A method of producing a nutritional supplement from microbial cells. The method includes cultivating microbial cells to obtain a biomass; incubating the biomass with a heat treatment at temperature from 55? C. up to 80? C. for an incubation time from 10 minutes up to 60 minutes; concentrating the biomass by separating and removing a liquid phase from a solid phase to obtain a dry matter content from 2% up to 40% of total weight of the nutritional supplement. Disclosed also is a system of producing a nutritional supplement from microbial cells using the aforementioned method. The system includes a bioreactor; a heat-exchanger and a separator for carrying out the respective steps of the aforementioned method.

Reference is made to the Identification of the Microorganism, with the identification reference given by the Depositor SoF1, having the Accession number given by the INTERNATIONAL DEPOSITORY AUTHORITY, VTT E-193585, received on Jun. 11, 2019

Provided herein is a composition comprising a solid product of a co-culture of bacteria and fungi, comprising at least 5% protein by dry weight and at least 5% fiber by dry weight. Further provided is a food article formed from a pellicle comprising a solid product of a co-culture of bacteria and fungi, which comprises at least 5% fungal protein by dry weight and at least 5% fiber by dry weight, and which is formed into a shape by cutting or molding. Further provided is a method of making such products by co-culturing bacteria and fungi to form a pellicle, and harvesting and shaping the pellicle. Further provided is a system that optimizes growth and production of a co-culture of bacteria and fungi (co-culture of microorganism) that includes a housing unit that includes stacked trays.

System and process are provided for producing and obtaining nutrient supplement products that are derived from microbial biomass from an anaerobic bacterial fermentation process using a myriad enzyme digestion and purification technique. More specifically, the disclosure provides process and system that simultaneously producing oxygenated hydrocarbon compounds at high specific productivity while effectively extracting nutrient supplement products from microbial biomass.

Provided herein are daily-like analogue compositions and the methods of making the same using one or more recombinant proteins.

A method of producing a meat analogue food ingredient. The method includes a downstream process including cultivating bacterial cells to obtain a biomass, separating a liquid phase and a solid phase of the biomass, concentrating the biomass by removing the liquid phase; and drying the biomass to obtain the first protein powder; mixing a first protein powder with a liquid and NaCl to obtain a powder mixture; extruding the powder mixture with high-moisture extrusion; cutting the extruded mixture; and cooling the extruded mixture.

Provided herein is a composition comprising a solid product of a co-culture of bacteria and fungi, comprising at least 5% protein by dry weight and at least 5% fiber by dry weight. Further provided is a food article formed from a pellicle comprising a solid product of a co-culture of bacteria and fungi, which comprises at least 5% fungal protein by dry weight and at least 5% fiber by dry weight, and which is formed into a shape by cutting or molding. Further provided is a method of making such products by co-culturing bacteria and fungi to form a pellicle, and harvesting and shaping the pellicle. Further provided is a system that optimizes growth and production of a co-culture of bacteria and fungi (co-culture of microorganism) that includes a housing unit that includes stacked trays.