A system and method for commercial production of aqueous Sargassum seaweed extract concentrate having arsenic level of around 1.32-15 mg/l; cadmium level of around 0.005-5 mg/l; lead level of around 0.2-12 mg/l; mercury level of around 0.00002-2 mg/l; and pH of around 7.4-9 is disclosed. The resulting seaweed extract concentrate can be used in plant and soil amendments, rooting hormones, other organic plant and soil amendments, organic fertilizers, adjuvants for use with organic pesticides, organic fungicides, in combinations of organic pesticides, with fungicides, and as fertilizers for agricultural, commercial and domestic use. The resulting seaweed extract concentrate can also be used to regulate plant growth, or as a plant nutrient. The method is believed to be able to prepare an extract concentrate from any high protein biomass, including Sargassum, water hyacinth or other seaweed.

The present disclosure relates to disrupting algae cell walls, in order to obtain protoplast with high nutrient digestibility for use as a food ingredient and/or as feed for farmed fish and shellfish species, which leads to the production of added value sea food items and to more sustainable and better performing food chains. The present disclosure described a method for obtaining proteins or a rich-protein extract from algae, comprising the steps of: disrupting a cell wall of algae by physical-mechanical means using a vibratory grinding mill with rings; submitting the disrupted algae to an enzymatic hydrolysis carried out with a mixture of enzymes; wherein the enzyme mixture is a mixture of at least two enzymes selected from the following list: lipase, pectinase, cellulase, hemicellullase, endo exo-arabanase, amylase, or mixtures thereof; provided that at least one of the enzymes is lipase, pectinase, amylase.

A system and method for commercial production of aqueous Sargassum seaweed extract concentrate having arsenic level of around 1.32-15 mg/l; cadmium level of around 0.005-5 mg/l; lead level of around 0.2-12 mg/l; mercury level of around 0.00002-2 mg/l; and pH of around 7.4-9 is disclosed. The resulting seaweed extract concentrate can be used in plant and soil amendments, rooting hormones, other organic plant and soil amendments, organic fertilizers, adjuvants for use with organic pesticides, organic fungicides, in combinations of organic pesticides, with fungicides, and as fertilizers for agricultural, commercial and domestic use. The resulting seaweed extract concentrate can also be used to regulate plant growth, or as a plant nutrient. The method is believed to be able to prepare an extract concentrate from any high protein biomass, including Sargassum, water hyacinth or other seaweed.

The invention relates to a method for preparing a protein isolate of the biomass of microalgae of the genus Chlorella, characterised in that it comprises the following steps: supplying a microalgal biomass produced by fermentation, washing the biomass so as to eliminate the soluble interstitial compounds and concentrating the biomass, mechanically grinding the washed and concentrated biomass in a horizontal ball grinder-type system in order to produce an emulsion, destructuring the emulsion thus produced, triple-phase separation so as to separate the soluble fraction from the fractions containing the lipids and the cell debris, recovery of the soluble fraction thus produced in order to produce the soluble protein isolate, then evaporation, pasteurisation and atomisation of said protein isolate.

A method for separation of proteins, particularly plant proteins, from salts and phenolic compounds in a liquid comprising said proteins dissolved in said liquid is provided. The method includes a step of subjecting said liquid to a first cross-flow membrane filtration process wherein the first salts and at least a portion of the phenolic compounds migrate across the membrane into a first permeate and the proteins are retained in a first retentate; followed by a step of adding water, or one or more second salts and water to the first retentate, while continuing the membrane filtration process, to create a diafiltrate containing at least a portion of said phenolic compounds and the added second salts.

The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of, e.g., chlorophyll and may thus provide lightening the color of the protein-enriched extracts. The methods generally include treatment with peracetic acid or hydrogen peroxide and filtrations. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant material.

The invention relates to a method for preparing a protein isolate of the biomass of microalgae of the genus Chlorella, characterised in that it comprises the following steps: supplying a microalgal biomass produced by fermentation, washing the biomass so as to eliminate the soluble interstitial compounds and concentrating the biomass, mechanically grinding the washed and concentrated biomass in a horizontal ball grinder-type system in order to produce an emulsion, destructuring the emulsion thus produced, triple-phase separation so as to separate the soluble fraction from the fractions containing the lipids and the cell debris, recovery of the soluble fraction thus produced in order to produce the soluble protein isolate, then evaporation, pasteurisation and atomisation of said protein isolate.

It has been found according to the invention, that a biomass containing an oxidation-sensitive material of value may be dried under particularly mild conditions by a method in which the drying gas is passed over the biomass to be dried in cycle gas mode.

A method for preparing a protein isolate of the biomass of microalgae of the genus Chlorella, includes the following steps: supplying a microalgal biomass produced by fermentation, washing the biomass so as to eliminate the soluble interstitial compounds and concentrating the biomass, mechanically grinding the washed and concentrated biomass in a horizontal ball grinder-type system in order to produce an emulsion, destructuring the emulsion thus produced, triple-phase separation so as to separate the soluble fraction from the fractions containing the lipids and the cell debris, recovery of the soluble fraction thus produced in order to produce the soluble protein isolate, then evaporation, pasteurization and atomization of the protein isolate.

The present disclosure is in the field of pharmacognosy and chemistry of natural products. The present disclosure generally relates to a process of isolation and purification of Biochemical Constituents from algae. The present disclosure particularly relates to a process of isolation and purification of Biochemical Constituents from a biomass of cyanobacteria. The present disclosure provides a process for isolating and extracting phycocyanins, chlorophylls, proteins and polysaccharides from the spirulina biomass.