A method for preparing a protein isolate of the biomass of microalgae of the genus Chlorella, includes the following steps: supplying a microalgal biomass produced by fermentation, washing the biomass so as to eliminate the soluble interstitial compounds and concentrating the biomass, mechanically grinding the washed and concentrated biomass in a horizontal ball grinder-type system in order to produce an emulsion, destructuring the emulsion thus produced, triple-phase separation so as to separate the soluble fraction from the fractions containing the lipids and the cell debris, recovery of the soluble fraction thus produced in order to produce the soluble protein isolate, then evaporation, pasteurization and atomization of the protein isolate.

The present invention provides a protein material and food ingredient from a sustainable and stable source. The sustainable and stable source of the food or food ingredient is biomass, for example an algal or microbial biomass. The invention discloses that the biomass can be subjected to a series of steps to derive the protein material and food or food ingredient, which has high nutritional content without the unacceptable organoleptic properties that typically accompany proteins and food ingredients from these sources.

Disclosed is a non-toxic omega-3 rich extract and non-toxic miscella and a method of producing same. The method may include obtaining an aqueous microalgae slurry comprising at least 65% water; mixing the aqueous microalgae slurry with ethanol for a predetermined duration; separating the aqueous microalgae slurry-ethanol mixture to liquids and miscella; and evaporating the water and the ethanol from the liquid to receive a liquid extract. In some embodiments, the miscella contains organic material and ash at an amount below 15 dry weight %.

The present disclosure relates to a Schizochytrium sp. Strain, and a method for producing a biomass by using the strain.

A protein preparation may be obtained from dried lipid extracted marine algae. The novel hydrolysate has high solubility and digestibility, low ash content, and high protein content (measured as g amino acid/g of product), with an essential amino acid profile that makes an excellent protein supplement for human consumption. The process utilizes a hydrolyzation reaction at high solids content to minimize water footprint and ash content, while increasing its solubility and digestibility.

Disclosed herein is a plant-based, blood-free diet formulation for hematophagous insects comprising effective amounts of a plant-based protein source and, in some aspects, a carbohydrate source, wherein the plant-based, blood-free diet formulation is suitable for egg production and colony maintenance of hematophagous insects. In some aspects, such hematophagous insects include, but are not limited to, mosquitoes. In some aspects, the plant-based protein source is derived from hemp seeds. In some aspects, the protein source comprises additional proteins in addition to hemp protein. In some aspects, one or more additional proteins in the formulation is derived from spirulina, soybeans, chia seeds, quinoa, oats, nutritional yeast, and/or green peas.

The present invention relates to a method for protein enrichment of a biomass of red unicellular algae such as Galdieria and to the biomass thus obtained.