The present invention discloses a native rapeseed protein isolate comprising 40 to 65% cruciferins and 35 to 60% napins and having a solubility of at least 88% when measured over a pH range from 3 to 10 at a temperature of 23±2° C.; wherein the native rapeseed protein isolate comprises from 5% to 65% on dry matter of 12S rapeseed protein where the presence of 12S is verified by Blue Native PAGE.

The present invention discloses a native rapeseed protein isolate comprising 40 to 65% cruciferins and 35 to 60% napins and having a solubility of at least 88% when measured over a pH range from 3 to 10 at a temperature of 23±2° C.; wherein the native rapeseed protein isolate comprises from 5% to 65% on dry matter of 12S rapeseed protein where the presence of 12S is verified by Blue Native PAGE.

A composition and process for making pet food treats is described herein. Auxiliary ingredients are combined to form a meat mixture. The meat mixture is formed into portions. The portions of meat mixture are positioned on a chew stick that comprises rawhide. The pet treat gives the appearance of a grilled shish kabob, where the meat portions are meant for initial taste, while the chew stick will provide the dog with a longer-lasting chewing portion.

A composition and process for making pet food treats is described herein. Auxiliary ingredients are combined to form a meat mixture. The meat mixture is formed into portions. The portions of meat mixture are positioned on a chew stick that comprises rawhide. The pet treat gives the appearance of a grilled shish kabob, where the meat portions are meant for initial taste, while the chew stick will provide the dog with a longer-lasting chewing portion.

A corn cob type pet chew where the corn cob has improved digestibility. The improved digestibility is provided by digestive enzymes and/or incorporating selected locations of mechanical weaking so that the corn cob will also more readily reduce in size when chewed upon by the animal. The corn cob may also optionally include other additives, such as mineral fillers. The minerals may also assist in digestion.

A corn cob type pet chew where the corn cob has improved digestibility. The improved digestibility is provided by digestive enzymes and/or incorporating selected locations of mechanical weaking so that the corn cob will also more readily reduce in size when chewed upon by the animal. The corn cob may also optionally include other additives, such as mineral fillers. The minerals may also assist in digestion.

Methods of identifying canine subjects having an increased likelihood of developing elevated levels of 4-ethylphenyl sulfate, canine stress, canine anxiety and/or an inhibition of growth of beneficial microbes and promotion of growth of harmful microbes are disclosed. Methods comprise analyzing a biological sample obtained from the canine subject for the presence of two copies of a minor allele of the single nucleotide polymorphism BICF2P1175095 in a canine subject. Methods of treating the identified canine subjects by administering an effective amount of tomato pomace are also disclosed. Methods of treating canine subjects for elevated 4-EPS levels, canine anxiety or canine stress are disclosed. Canine food compositions that comprises tomato pomace are disclosed.

Methods of identifying canine subjects having an increased likelihood of developing elevated levels of 4-ethylphenyl sulfate, canine stress, canine anxiety and/or an inhibition of growth of beneficial microbes and promotion of growth of harmful microbes are disclosed. Methods comprise analyzing a biological sample obtained from the canine subject for the presence of two copies of a minor allele of the single nucleotide polymorphism BICF2P1175095 in a canine subject. Methods of treating the identified canine subjects by administering an effective amount of tomato pomace are also disclosed. Methods of treating canine subjects for elevated 4-EPS levels, canine anxiety or canine stress are disclosed. Canine food compositions that comprises tomato pomace are disclosed.

Methods of analyzing a biological sample obtained from the canine subject for the presence of one or two copies one or two copies of minor allele T of SNP Affx-206229307; or one or two copies of minor allele A of SNP Affx-206560187; or one or two copies of minor allele T of SNP Affx-206229307 and one or two copies of minor allele A of SNP Affx-206560187 are disclosed. The methods are used in methods to identify a canine subject that has an increased likelihood or risk of hypothyroidism and in methods of treating a canine subject to reduce risk of hypothyroidism or to treat a canine subject that has hypothyroidism. The treatments comprise administering to the canine subject a low arginine diet and/or comprising an effective amount of a composition comprising a protein source, a carbohydrate source, a vegetable source, and a fruit source. Canine food compositions are disclosed.

Methods of analyzing a biological sample obtained from the canine subject for the presence of one or two copies one or two copies of minor allele T of SNP Affx-206229307; or one or two copies of minor allele A of SNP Affx-206560187; or one or two copies of minor allele T of SNP Affx-206229307 and one or two copies of minor allele A of SNP Affx-206560187 are disclosed. The methods are used in methods to identify a canine subject that has an increased likelihood or risk of hypothyroidism and in methods of treating a canine subject to reduce risk of hypothyroidism or to treat a canine subject that has hypothyroidism. The treatments comprise administering to the canine subject a low arginine diet and/or comprising an effective amount of a composition comprising a protein source, a carbohydrate source, a vegetable source, and a fruit source. Canine food compositions are disclosed.