A device for performing a microfluidic assay on a chip comprising, a microfluidics chip, one or more fluid receptacles on the chip for receiving a fluid, a plurality of pneumatic pumps arrayed on the chip, each pump having a discharge channel leading to a rectifier on the chip, and a reaction chamber in fluid communication with each of the rectifiers, wherein a pressure on the pressurized fluid source drives fluid from the fluid receptacle into the incoming fluid channel connecting the fluid receptacle to the pump, through the pump and into the discharge channel, through the discharge channel to the rectifier, and through the rectifier into the reaction chamber, wherein the pump is configured to generate droplets of a pre-determined size, wherein the rectifiers prevent backflow of the droplets, and wherein droplets are combined in the reaction chamber, the chamber facilitating an assay being performed on the chip.

The present invention relates to a microfluidic assay system and associated reading device, as well as the individual components themselves. The present invention also relates to methods of conducting assays, using a disposable system and associated reading device, as well as kits for conducting assays.

The present invention relates to a microfluidic assay system and associated reading device, as well as the individual components themselves. The present invention also relates to methods of conducting assays, using a disposable system and associated reading device, as well as kits for conducting assays.

An apparatus for creating a flow of gas comprises a frame having a passage defined therethrough. The passage extends a length along a central longitudinal axis from an inlet to an outlet and has a first side and a second side. A flexible pumping membrane is disposed within the passage. The membrane has a first edge coupled to the first side of the passage at a midline thereof and a second edge, disposed opposite the first edge, coupled to the second side of the passage at a midline thereof. The membrane segregates the frame into an upper portion and a lower portion. The apparatus also includes an actuating system which is structured to selectively move portions of the membrane toward either the upper portion or the lower portion of the frame in a manner which causes a wave-like movement in the pumping membrane and creates the flow of gas.

An apparatus for creating a flow of gas comprises a frame having a passage defined therethrough. The passage extends a length along a central longitudinal axis from an inlet to an outlet and has a first side and a second side. A flexible pumping membrane is disposed within the passage. The membrane has a first edge coupled to the first side of the passage at a midline thereof and a second edge, disposed opposite the first edge, coupled to the second side of the passage at a midline thereof. The membrane segregates the frame into an upper portion and a lower portion. The apparatus also includes an actuating system which is structured to selectively move portions of the membrane toward either the upper portion or the lower portion of the frame in a manner which causes a wave-like movement in the pumping membrane and creates the flow of gas.

Disclosed is a dual-stage fluidics system that has reduced pulsation. This system uses inexpensive peristaltic pumps and two reservoirs that dampen pulsations in pressure. Peristaltic pumps are used as air pressure pumps and sheath fluid pumps that are placed between the two pressurized reservoirs and isolate the two reservoirs. Since the two reservoirs are maintained at a substantially even pressure, pulsations are reduced so that the peristaltic pumps essentially provide air flow and sheath fluid flow as needed.

Disclosed is a dual-stage fluidics system that has reduced pulsation. This system uses inexpensive peristaltic pumps and two reservoirs that dampen pulsations in pressure. Peristaltic pumps are used as air pressure pumps and sheath fluid pumps that are placed between the two pressurized reservoirs and isolate the two reservoirs. Since the two reservoirs are maintained at a substantially even pressure, pulsations are reduced so that the peristaltic pumps essentially provide air flow and sheath fluid flow as needed.

A linear cleaning peristaltic metering pump includes a pump body, a hose fixing accessory and an anti-backflow accessory. The hose fixing accessory is configured for arranging a working hose. The anti-backflow accessory is configured for clamping and loosening the working hose. The pump body is configured for performing reciprocating motion to clamp or loosen the working hose, thereby realizing a pumping effect by metering pump. The anti-backflow accessory is in cooperation with the pump body. The pump body comprises a roller lifting assembly, a roller hose-pressing assembly, a pump body outer cover assembly and a power transmission part. The power transmission part is configured for driving the roller lifting assembly to perform lifting linear motion, thereby driving the roller hose-pressing assembly to move and to clamp or loosen the working hose.

A linear cleaning peristaltic metering pump includes a pump body, a hose fixing accessory and an anti-backflow accessory. The hose fixing accessory is configured for arranging a working hose. The anti-backflow accessory is configured for clamping and loosening the working hose. The pump body is configured for performing reciprocating motion to clamp or loosen the working hose, thereby realizing a pumping effect by metering pump. The anti-backflow accessory is in cooperation with the pump body. The pump body comprises a roller lifting assembly, a roller hose-pressing assembly, a pump body outer cover assembly and a power transmission part. The power transmission part is configured for driving the roller lifting assembly to perform lifting linear motion, thereby driving the roller hose-pressing assembly to move and to clamp or loosen the working hose.

Peristaltic micropump assemblies configured to pump fluid from a patient and/or deliver pharmaceutical agents to the patient are provided. In some embodiments, a micropump assembly can include a rotor configured to rotate about an axis and comprising a compressing member configured to rotate along a circumference, and a fluid chamber positioned at least partially around the circumference. The fluid chamber includes a round outer ring and a membrane attached to the outer ring and opposing an inner face of the outer ring. The compressing member is configured to compress the fluid chamber and move a fluid through the fluid chamber. The assembly can fit within a housing that is sized and shaped to be implanted in the patient. For example, the micropump assembly can be inserted into the patient's ocular cavity and configured to displace fluid from the patient's eye.