The present disclosure relates to compositions and methods for enhancing mosquito feeding.

The present disclosure relates to compositions and methods for enhancing mosquito feeding.

The present invention provides an artificial diet for Orius strigicollis (O. strigicollis), a preparation method and use thereof belonging to the technical field of insect rearing wherein the artificial diet includes the following raw materials: compound amino acids, water, potassium sorbate, vitamin complex, folic acid, biotin, glucose, sodium chloride, yeast powder, sodium alginate, calcium lactate, and greenish yellow food coloring. The artificial diet of the present invention is beneficial for O. strigicollis to forage, promotes growth and development thereof, improves fecundity thereof, and increases the survival rate of O. strigicollis nymphs.

The present invention provides an artificial diet for Orius strigicollis (O. strigicollis), a preparation method and use thereof belonging to the technical field of insect rearing wherein the artificial diet includes the following raw materials: compound amino acids, water, potassium sorbate, vitamin complex, folic acid, biotin, glucose, sodium chloride, yeast powder, sodium alginate, calcium lactate, and greenish yellow food coloring. The artificial diet of the present invention is beneficial for O. strigicollis to forage, promotes growth and development thereof, improves fecundity thereof, and increases the survival rate of O. strigicollis nymphs.

Provided herein are agents, compositions, and methods for agricultural use, e.g., for altering the level, activity, or metabolism of one or more microorganisms resident in a host nematode or arthropod (e.g., honeybee or silkworm), the alteration resulting in an increase in the fitness of the host. The invention features a composition that includes an agent (e.g., phage, peptide, small molecule, antibiotic, or combinations thereof) that can alter the host's microbiota in a manner that is beneficial to the host. By promoting favorable microbial levels, microbial activity, microbial metabolism, and/or microbial diversity, the agents described herein may be used to increase the fitness of a variety of beneficial nematodes or arthropods, such as bees and silkworms, utilized in agriculture and commerce.

Provided herein are agents, compositions, and methods for agricultural use, e.g., for altering the level, activity, or metabolism of one or more microorganisms resident in a host nematode or arthropod (e.g., honeybee or silkworm), the alteration resulting in an increase in the fitness of the host. The invention features a composition that includes an agent (e.g., phage, peptide, small molecule, antibiotic, or combinations thereof) that can alter the host's microbiota in a manner that is beneficial to the host. By promoting favorable microbial levels, microbial activity, microbial metabolism, and/or microbial diversity, the agents described herein may be used to increase the fitness of a variety of beneficial nematodes or arthropods, such as bees and silkworms, utilized in agriculture and commerce.

A method for producing biogas includes: anaerobically digesting excrement with an additive, the additive including a pre-pupal stage of an insect from the scientific classification superfamily of Stratiomyoidea; and collecting the biogas. A method for producing an additive for anaerobic digestion of excrement includes: hatching eggs of an insect from the scientific classification superfamily of Stratiomyoidea in excrement; growing insect larvae by feeding with additional excrement; harvesting pre-pupal insect larvae; and grinding the pre-pupal larvae. An apparatus for producing an additive for anaerobic digestion of excrement includes: a tray having pivot along one lateral side and an upwardly sloping wall terminating with a lip along a lateral side opposite the pivot; a flume adjacent the lip; and a conveyor adjacent the pivot.

A method for producing biogas includes: anaerobically digesting excrement with an additive, the additive including a pre-pupal stage of an insect from the scientific classification superfamily of Stratiomyoidea; and collecting the biogas. A method for producing an additive for anaerobic digestion of excrement includes: hatching eggs of an insect from the scientific classification superfamily of Stratiomyoidea in excrement; growing insect larvae by feeding with additional excrement; harvesting pre-pupal insect larvae; and grinding the pre-pupal larvae. An apparatus for producing an additive for anaerobic digestion of excrement includes: a tray having pivot along one lateral side and an upwardly sloping wall terminating with a lip along a lateral side opposite the pivot; a flume adjacent the lip; and a conveyor adjacent the pivot.

The present invention provides for a probiotic composition for enhancing a subject's, such as an insect's, resistance to a pesticide comprising: (a) a microorganism capable of expressing one or more enzymes for metabolizing the pesticide, and (b) at least one feed ingredient for a subject. The present invention also provides for a method for enhancing a subject's, such as an insect's, resistance to a pesticide comprising administering to a subject the probiotic composition.

The present invention provides for a probiotic composition for enhancing a subject's, such as an insect's, resistance to a pesticide comprising: (a) a microorganism capable of expressing one or more enzymes for metabolizing the pesticide, and (b) at least one feed ingredient for a subject. The present invention also provides for a method for enhancing a subject's, such as an insect's, resistance to a pesticide comprising administering to a subject the probiotic composition.