A process for the production of fat-encapsulated follistatin. The process includes metering fat having a melting point of at least 40° C. into an extruder, heating the fat in the extruder during rotation of the at least one screw, metering the follistatin into the extruder barrel to produce a flowable mixture, cooling the flowable mixture in a downstream adjacent section of the extruder barrel, subsequently discharging the mixture through an extruder die, and comminuting the mixture after it discharges from the extruder die.

A packaged coffee product with oxygen dissolved therein along with milk and/or sweeteners in the sealed packaging is provided. Preferably, the pH of the product is 4.6 or greater, preferably 5.0 or greater. The coffee product may be hot or cold brew coffee and may have other gasses such as nitrogen and/or carbon-dioxide in the sealed container. Ideally, there is oxygen in both the headspace and liquid portion of the beverage in order to inhibit C. Bot growth without requiring retort processing. Preferably, the calorie count is in the range of 0.5-9 calories per ounce.

A packaged coffee product with oxygen dissolved therein along with milk and/or sweeteners in the sealed packaging is provided. Preferably, the pH of the product is 4.6 or greater, preferably 5.0 or greater. The coffee product may be hot or cold brew coffee and may have other gasses such as nitrogen and/or carbon-dioxide in the sealed container. Ideally, there is oxygen in both the headspace and liquid portion of the beverage in order to inhibit C. Bot growth without requiring retort processing. Preferably, the calorie count is in the range of 0.5-9 calories per ounce.

A hot-water sterilized package includes a container having a barrier layer filled with contents, wherein the barrier layer contains 96 mass % or more of a modified ethylene-vinyl alcohol copolymer based on the resin total, the modified ethylene-vinyl alcohol copolymer is represented by a following formula (I), contents (mol %) of a, b, and c based on the total monomer units satisfy following formulae (1) through (3), a degree of saponification (DS) defined by a following formula (4) is 90 mol % or more, and in measurement using a differential scanning calorimeter (DSC), crystalline melting enthalpy (ΔH.sub.A: J/g) during temperature rise in a hydrated state and crystalline melting enthalpy (ΔH.sub.B: J/g) during temperature rise after drying and melting followed by rapid cooling satisfy following formulae (5) and (6). A container constituting such a package has excellent in oxygen barrier properties even after hot-water sterilization, and thus degradation of content quality is inhibited for a long period. ##STR00001##
18≤a≤55  (1)
0.01≤c≤20  (2)
[100−(a+c)]×0.9≤b≤[100−(a+c)]  (3)
DS=[(Total Number of Moles of Hydrogen Atoms in X,Y, and Z)/(Total Number of Moles of X,Y, and Z)]×100  (4)
ΔH.sub.A/ΔH.sub.B≥0.5  (5)
ΔH.sub.B≥70  (6).

Methods of making mixed allergen products are provided, wherein the mixed allergen products are substantially free of replication viable organisms.

Provided is a simulation model sample for evaluation of heat treatment including a porous water absorbing material that is flexible and deformable; and a container that is configured to be able to contain the porous water absorbing material having water absorbed therein. Also provided is a method for evaluating heat treatment using a simulation model sample including a step of allowing a flexible and deformable porous water absorbing material to absorb water, and the porous water absorbing material to be contained in a container, to produce a simulation model sample; and a step of subjecting the simulation model sample to heat treatment, while measuring a temperature inside the simulation model sample.

A multilayer container for elevated temperature, high pressure processing of a material within the container, using a pressure transmission liquid within the container, the multilayer container having a container wall having an inner layer formed of a plastic for containing the liquid in contact with the inner side thereof and at least one further layer outboard of the inner layer and formed of different plastic from the inner layer, wherein the inner layer has a compression heating coefficient at least as high as the compression heating coefficient of the pressure transmission liquid in contact with the inner side thereof and the at least one further layer has a lower thermal conductivity at ambient pressure and temperature than the inner layer.

A multilayer container for elevated temperature, high pressure processing of a material within the container, using a pressure transmission liquid within the container, the multilayer container having a container wall having an inner layer formed of a plastic for containing the liquid in contact with the inner side thereof and at least one further layer outboard of the inner layer and formed of different plastic from the inner layer, wherein the inner layer has a compression heating coefficient at least as high as the compression heating coefficient of the pressure transmission liquid in contact with the inner side thereof and the at least one further layer has a lower thermal conductivity at ambient pressure and temperature than the inner layer.

Provided is a simulation model sample for evaluation of heat treatment including a porous water absorbing material that is flexible and deformable; and a container that is configured to be able to contain the porous water absorbing material having water absorbed therein. Also provided is a method for evaluating heat treatment using a simulation model sample including a step of allowing a flexible and deformable porous water absorbing material to absorb water, and the porous water absorbing material to be contained in a container, to produce a simulation model sample; and a step of subjecting the simulation model sample to heat treatment, while measuring a temperature inside the simulation model sample.

A method of heat-treatment of a product in a sealed container of a packaging material is described. The method includes placing the container in a treatment environment having a pressure (P) and a temperature (T), the pressure and the temperature having a ratio (P/T), and adjusting the temperature and/or pressure in the treatment environment to decrease the ratio over a duration in a time period. The time period starts at a time of cooking onset of the product and extends over a time of cooling onset at which time a temperature decrease is started, the time of cooking onset being preceded by a first time period of temperature ramping during which the temperature is increased. An apparatus for controlling heat-treatment of a product in a sealed container of a packaging material is also described.