A cooler box with a temperature control function, the cooler box comprises a box body and a cryogenic medium container installed outside the cooler box for storing liquid carbon dioxide or other liquefied gas. The box body is equipped with a controller, a monitoring device, a controllable valve, and a chamber to reduce the speed of gas released and thus prevent sudden freezing and overcooling of temperature-sensitive cargo such as fresh food. The controllable valve is connected to the cryogenic medium container through a tube. The monitoring device measures the temperature inside the cooler box, and the controller controls the valve opening state and the duration time of maintaining the open state of the controllable valve according to the temperature measured by the monitoring device and the size of the cooler box. A temperature control method for the cooler box is also provided.

The invention relates to a method and an apparatus for preparing a cryogenic sample, whereby the sample is subjected to rapid cooling using a cryogen. A pair of conduits for transporting cryogenic fluid are provided, each of which conduits opens out into a mouthpiece, which mouthpieces are arranged to face each other across an intervening gap, wherein in said gap a sample that is provided on a substantially planar sample carrier can be received. Cryogenic fluid can be pumped through said conduits so as to concurrently flush from said mouthpieces and suddenly immerse the sample in cryogenic fluid from two opposite sides. As defined herein, at least one of said mouthpieces comprises at least two nozzle openings for evenly cooling said substantially planar sample carrier during said flushing.

The invention relates to a method and an apparatus for preparing a cryogenic sample, whereby the sample is subjected to rapid cooling using a cryogen. A pair of conduits for transporting cryogenic fluid are provided, each of which conduits opens out into a mouthpiece, which mouthpieces are arranged to face each other across an intervening gap, wherein in said gap a sample that is provided on a substantially planar sample carrier can be received. Cryogenic fluid can be pumped through said conduits so as to concurrently flush from said mouthpieces and suddenly immerse the sample in cryogenic fluid from two opposite sides. As defined herein, at least one of said mouthpieces comprises at least two nozzle openings for evenly cooling said substantially planar sample carrier during said flushing.

A fluid storage tank can be configured to store a cooling fluid in a liquid state and a gas state. A first heat exchanger can be configured to release heat into the fluid storage tank. A second heat exchanger can be disposed fluidly downstream of the fluid storage tank and configured to exchange heat between the cooling fluid and a heat load. A pressure control device can be disposed fluidly downstream of the second heat exchanger. The first heat exchanger can be fluidly downstream of the second heat exchanger such that cooling fluid, after being heated in the second heat exchanger, passes through the first heat exchanger and thereby heats upstream cooling fluid resident in the fluid storage tank.

This cryogenic preservation or vitrification device (1) comprises: an accumulator (2) including a first volume of pressurised hydraulic oil; a control system controlling a valve (20) capable of releasing a second volume of hydraulic oil from the accumulator toward a cylinder (55) by means of a pipe (4); a cylinder (55) including a piston (6) configured to be driven by the second volume of hydraulic oil and to displace a first volume of cryogenic fluid to a cryogenic vessel (7) intended to receive a sample (8) to be cryogenically preserved.

This cryogenic preservation or vitrification device (1) comprises: an accumulator (2) including a first volume of pressurised hydraulic oil; a control system controlling a valve (20) capable of releasing a second volume of hydraulic oil from the accumulator toward a cylinder (55) by means of a pipe (4); a cylinder (55) including a piston (6) configured to be driven by the second volume of hydraulic oil and to displace a first volume of cryogenic fluid to a cryogenic vessel (7) intended to receive a sample (8) to be cryogenically preserved.

A fluid storage tank can be configured to store a cooling fluid in a liquid state and a gas state. A first heat exchanger can be configured to release heat into the fluid storage tank. A second heat exchanger can be disposed fluidly downstream of the fluid storage tank and configured to exchange heat between the cooling fluid and a heat load. A pressure control device can be disposed fluidly downstream of the second heat exchanger. One of the first cooling fluid that has been heated by the second heat exchanger or a second cooling fluid different than the first cooling fluid can pass through the first heat exchanger and thereby heat upstream first cooling fluid resident in the fluid storage tank.

An instant freezer apparatus able to freeze consumable fluids and food having a freezing point lower than water is disclosed. The method of the present invention is directed to freezing of a substance such as to instantly freeze consumable fluids and food without altering their chemical compositions. The instant freezer apparatus generally comprises a main frame and a removable freezing module. The apparatus may further comprise a freezing fluid injection system such as a liquid carbon dioxide tank or a liquid nitrogen tank fluidly connected to the main frame. At least two freezing cells comprising the fluid to be frozen may be included, the freezing cells being independently removable from the freezing module and other freezing cells. A semi-flexible mat for receiving and supporting varying shapes of freezing cells may be included.

An instant freezer apparatus able to freeze consumable fluids and food having a freezing point lower than water is disclosed. The method of the present invention is directed to freezing of a substance such as to instantly freeze consumable fluids and food without altering their chemical compositions. The instant freezer apparatus generally comprises a main frame and a removable freezing module. The apparatus may further comprise a freezing fluid injection system such as a liquid carbon dioxide tank or a liquid nitrogen tank fluidly connected to the main frame. At least two freezing cells comprising the fluid to be frozen may be included, the freezing cells being independently removable from the freezing module and other freezing cells. A semi-flexible mat for receiving and supporting varying shapes of freezing cells may be included.

The present invention provides a sub-zero treatment device capable of uniformly cooling a cooling target object and reducing the amount of liquid refrigerant used for cooling the cooling target object. The sub-zero treatment device has an exhaust member extending from a through-hole provided in a cooling tank constituting a cooling target object mounting chamber through to the interior of the cooling target object mounting chamber, and having an exhaust port, wherein the exhaust port is disposed in an exhaust port positioning space, which is the space located in the upper half of the cooling target object mounting chamber and having a width in the transverse direction that is equal to the maximum width in the transverse direction of the suction port.