The present disclosure provides cryogenic storage systems and methods of using the cryogenic storage systems. A cryogenic storage system of the present disclosure may comprise a cryogenic tank with an inner door and an outer door, and a robot apparatus located adjacent to the cryogenic tank. The cryogenic tank may store multiple racks such that at most a single rack is removable through the inner door or the outer door. The cryogenic tank may store the multiple racks in multiple groups of racks comprising a first group of racks located at a first radial distance and a second group of racks located at a second radial distance that is greater than the first radial distance. The robot apparatus may selectively open and close the inner or outer doors, and insert or withdraw the single rack into or out of the cryogenic tank through the inner door or the outer door.

A cooling system for a cryochamber comprises compressors, heat exchangers and flow restrictions. Compressors are used to pressurize the working fluid, heat exchangers are used to release the heat to the ambient, absorb heat from the interior of the cryochamber to decrease the temperature inside the cryochamber, to cool the working fluid below the ambient temperature using source of cold or to recuperate heat of the cold working fluid stream. Flow restrictions are used to decrease the pressure of the working fluid which results in its temperature decrease. Cryochambers are used for whole-body cryotherapy and require cooling systems that are able to lower the air temperature inside the cryochamber to −90° F.

A railcar backup cooling system may be added to an existing railcar (e.g., a boxcar) to provide supplemental or backup cooling to the railcar in the event that an HVAC failure or other circumstance occurs, which causes the interior temperature of the railcar to rise. The system includes a container of liquid or compressed gas mounted on the railcar, a valve controlling the flow of the liquid or gas from the container, and a controller configured to open the valve when the HVAC system fails or is otherwise unable to maintain the railcar at the desired temperature. When the valve is opened, the liquid and/or gas stored in the container may exit, expanding into a cool gas and thereby acting to cool the railcar environment.

The invention relates to a method and an apparatus for preparing a cryogenic sample, whereby the sample is subjected to rapid cooling using a cryogen. A pair of conduits for transporting cryogenic fluid are provided, each of which conduits opens out into a mouthpiece, which mouthpieces are arranged to face each other across an intervening gap, wherein in said gap a sample that is provided on a substantially planar sample carrier can be received. Cryogenic fluid can be pumped through said conduits so as to concurrently flush from said mouthpieces and suddenly immerse the sample in cryogenic fluid from two opposite sides. As defined herein, at least one of said mouthpieces comprises at least two nozzle openings for evenly cooling said substantially planar sample carrier during said flushing.

A fluid storage tank can be configured to store a cooling fluid in a liquid state and a gas state. A first heat exchanger can be configured to release heat into the fluid storage tank. A second heat exchanger can be disposed fluidly downstream of the fluid storage tank and configured to exchange heat between the cooling fluid and a heat load. A pressure control device can be disposed fluidly downstream of the second heat exchanger. The first heat exchanger can be fluidly downstream of the second heat exchanger such that cooling fluid, after being heated in the second heat exchanger, passes through the first heat exchanger and thereby heats upstream cooling fluid resident in the fluid storage tank.

This cryogenic preservation or vitrification device (1) comprises: an accumulator (2) including a first volume of pressurised hydraulic oil; a control system controlling a valve (20) capable of releasing a second volume of hydraulic oil from the accumulator toward a cylinder (55) by means of a pipe (4); a cylinder (55) including a piston (6) configured to be driven by the second volume of hydraulic oil and to displace a first volume of cryogenic fluid to a cryogenic vessel (7) intended to receive a sample (8) to be cryogenically preserved.

An apparatus for cryostorage and manipulation of a plurality of container units includes a cryochamber having a cryo-access port. The cryochamber is electrically cooled at cryogenic temperatures. A unit holder is located inside the cryochamber and is configured to hold a plurality of container units. A user access area is provided for selectively permitting access to a chosen container unit by an authenticated user who has been authenticated by the apparatus. A motive grasper is provided for selectively removing the chosen container unit from the cryochamber through the cryo-access port, and selectively placing the chosen container unit into the user access area.

A fluid storage tank can be configured to store a cooling fluid in a liquid state and a gas state. A first heat exchanger can be configured to release heat into the fluid storage tank. A second heat exchanger can be disposed fluidly downstream of the fluid storage tank and configured to exchange heat between the cooling fluid and a heat load. A pressure control device can be disposed fluidly downstream of the second heat exchanger. One of the first cooling fluid that has been heated by the second heat exchanger or a second cooling fluid different than the first cooling fluid can pass through the first heat exchanger and thereby heat upstream first cooling fluid resident in the fluid storage tank.

In some embodiments, systems and methods are provided that limit the change in temperature and/or control a temperature of a product during delivery. Some embodiments provide systems to limit temperature changes, comprising: an evaporative product cooling system comprising: a product cavity that supports a product while the product is transported to a delivery location, wherein the product cooling system comprises an interior wall defining the product cavity, an exterior wall, an evaporative cavity between the interior and exterior walls, a coolant dispensing system, at least one evaporative opening, and a temperature sensor; and a temperature control circuit configured to receive temperature data from the temperature sensor while the product is in transit, determine that a temperature of the product is greater than a transport temperature threshold, and autonomously activate the coolant dispensing system to release evaporative coolant into the evaporative cavity while the product is transported.

High-pressure cryogenic fluid conduits that deliver high-pressure cryogenic fluids from a first point to a second point. This high-pressure fluid conduit has a safety feature that is activated when the high-pressure cryogenic fluid conduit fails due to exposure to a predetermined force. The safety feature is activated by the fracture of an annular ring that is positioned at either end of the high-pressure fluid conduit and is calibrated to fracture when exposed to the predetermined force. Fracture of the annular ring closes valves at each end of the high-pressure fluid conduit, thereby stopping the flow of high-pressure fluid from the high-pressure fluid source as well as the escape of high-pressure fluid from the high-pressure fluid container.