The present invention relates to a method for processing fresh algae at ambient temperature by subjecting algae to an osmotic shock and treating the disrupted algae with an enzyme composition comprising cell wall degrading enzymes. This gentle process at ambient temperature allows for the isolation of algal protein which has good solubility, also in the presence of salt and good foaming, emulsifying and water binding properties. Another advantage is that this method of protein isolation allows for cascaded biorefinery, since protein isolation may be followed by a treatment of the remaining biomass with carbohydrate degrading enzymes to produce clean biogas in high yields and a mineral rich water stream in anaerobic digestion.

Set forth herein is a composition that includes a fungal pellet and animal cells, wherein the animal cells are in connection with the fungal pellet. The animal cells can be located on the exterior surface of the fungal pellet, the interior of the fungal pellet, or both. The fungal pellet can include intact fungal cells. The fungal pellet can be substantially inviable. In an example, the pellet can be heat-treated, chemically treated, or lyophilized. The animal cells can be mammalian cells, wherein the mammalian cells may be non-human mammalian cells.

The present invention contemplates the creation of a low fluoride crustacean oil processed from a phospholipid-protein complex (PPC) formed immediately upon a crustacean (i.e., for example, krill) catch. Further, the crustacean oil may also have reduced trimethyl amine and/or trimethyl amino oxide content. The process comprises disintegrating the crustaceans into smaller particles, adding water, heating the result, adding enzyme(s) to hydrolyze the disintegrated material, deactivating the enzyme(s), removing solids from the enzymatically processed material to reduce fluoride content of the material, separating and drying the PPC material. Then, using extraction with supercritical CO.sub.2 or supercritical dimethyl ether, and/or ethanol as solvents, krill oil, inter alia, is separated from the PPC. In the extraction the krill oil can be separated almost wholly from the feed material.

A method of processing whole fresh shellfish, including whole live bivalves, and the resulting compositions in liquid and dried form having a high yield of bioactive components with improved bioavailability. The method includes an enzyme treatment step of applying an enzyme formulation having one or more enzymes to the shellfish, and leaving the shellfish in contact with the enzyme formulation until the flesh and other biological material is substantially separated from the shells or exoskeletons of the shellfish, and liquefying the flesh and biological material using of the same enzyme formulation in the same enzyme treatment step, and/or by applying a different enzyme formulation in the same or one or more subsequent enzyme treatment steps. In a preferred method the shellfish starting material is live at the application of the enzyme. The method does not use mechanical processes to reduce the size of the shellfish before application of the enzyme.

Provided are methods of making high quality seaweed meal suitable for food applications. The method comprises the steps of cleaning the fresh, dried, or rehydrated red seaweed, pretreating the red seaweed with a salt under heat, and drying and grinding the treated red seaweed to obtain the seaweed meal. Optionally, the method comprises bleaching the red seaweed, and/or hydrolyzing the red seaweed with cellulase. The seaweed meal produced by the disclosed technology has a light color, good flavor and taste, good mouthfeel, high gelling capability, great stability, and ideal smoothness and slipperiness suitable for food applications.

Provided are methods of making high quality seaweed meal suitable for food applications. The method comprises the steps of cleaning the fresh, dried, or rehydrated red seaweed, pretreating the red seaweed with alkali, pre-treating the red seaweed with acid, hydrolyzing the red seaweed with cellulase, and drying and grinding the treated red seaweed to obtain the seaweed meal. The seaweed meal produced by the disclosed technology has light color, good flavor and taste, good mouthfeel, high gelling capability, great stability, and ideal smoothness and slipperiness suitable for food applications.

The present disclosure provides an organic selenium enriched edible marine microalga Nannochloropsis oceanica CASA CC201 with essential micronutrient Selenium, comprising organic selenium, along with omega 3 fatty acid and mono unsaturated fatty acid (MUFA). The present disclosure also provides a process for the organic selenium enrichment of edible marine microalga and a composition comprising the organic selenium enriched edible marine microalga Nannochloropsis oceanica. Moreover, the present disclosure also discloses a composition comprising the sustained bioprocess for organic selenium enriched edible marine microalga Nannochloropsis oceanica selenium enriched edible marine microalga, and wherein the composition is in the form of food, health, and feed supplements.

The patent discloses halophilic bacteria and a method for fermenting fish paste, belonging to the technical field of solid-state fermentation of aquatic products using microorganisms. Four kinds of screened halophilic bacteria were used as mixed starter culture to produce fish paste. The effects of the ratio of mixed starter culture, inoculation amount, fermentation temperature, fermentation time and salinity on fish paste were studied by determining physical and chemical indexes and performing sensory evaluation. The fish paste has the characteristics of high safety, low salinity, high flavor and nutrition value. This method of fish paste processing needs short fermentation time. The fermented products of fish paste with better flavor can be obtained by using the mixed starter culture.

Provided is an enzyme composition that can be used to produce food products with less contaminants and excellent flavor and texture. An enzyme composition for food products containing a peptidase and having substantially no contaminating activity.

The present invention discloses a method for the preparation of skipjack tuna extract having hypouricemic effect and the use thereof, which is simple in processing operations, low in production cost, and is free from pollution. The prepared skipjack tuna extract has a potent hypouricemic effect, and has a significant therapeutic effect on hyperuricemia, with no toxic and side effects. The method in an example of the present invention comprises: pretreating skipjack tuna to obtain a skipjack tuna slurry; enzymolysing the skipjack tuna slurry to obtain a crude enzymolysis liquid; removing fishy smell and bitter taste, removing impurities by activated charcoal, and filtering to obtain a refinded enzymolysis liquid; concentrating under vacuum and spray-drying to obtain the skipjack tuna extract. The present invention further discloses use of the skipjack tuna extract in health care products or food products.