Methods of using split G-quadruplexes associated with functional tags for associating said tags to target nucleic acids. Methods include use of split G-quadruplexes associated with detection tags for the detection of target nucleic acids, and use of split G-quadruplexes associated with capture tags for detection or capture of target nucleic acids.

An example valve assembly includes a first workport fluidly coupled to a first actuator; a second workport fluidly coupled to the first actuator; a third workport fluidly coupled to a second actuator, wherein the third workport is fluidly coupled to the first workport via a first fluid passage; a fourth workport fluidly coupled to the second actuator, wherein the fourth workport is fluidly coupled to the second workport via a second fluid passage; and a spool axially movable in a bore within the valve assembly, wherein when the spool is shifted axially in a first axial direction, pressurized fluid is provided to the first workport and to the third workport via the first fluid passage, and when the spool is shifted axially in a second axial direction opposite the first axial direction, pressurized fluid is provided to the second workport and to the fourth workport via the second fluid passage.

An example valve assembly includes a first workport fluidly coupled to a first actuator; a second workport fluidly coupled to the first actuator; a third workport fluidly coupled to a second actuator, wherein the third workport is fluidly coupled to the first workport via a first fluid passage; a fourth workport fluidly coupled to the second actuator, wherein the fourth workport is fluidly coupled to the second workport via a second fluid passage; and a spool axially movable in a bore within the valve assembly, wherein when the spool is shifted axially in a first axial direction, pressurized fluid is provided to the first workport and to the third workport via the first fluid passage, and when the spool is shifted axially in a second axial direction opposite the first axial direction, pressurized fluid is provided to the second workport and to the fourth workport via the second fluid passage.

A four-position switching valve includes first and second pistons for driving a spool in a valve body, and a spool moving mechanism part that moves the spool to first and second intermediate switching positions between one-end side and the other-end-side switching positions. The spool moving mechanism part includes a compression spring that moves the spool back in the opposite direction by moving to the switching position on one end side and the other end side of the spool. The compression spring moves the spool to the first intermediate switching position when the spool moves to one-end-side switching position and the pressure on the second piston is released, and moves the spool to the second intermediate switching position when the spool moves to the other-end-side switching position and the pressure on the first piston is released.

A hydrogen gas supply device supplies hydrogen gas to a fuel cell stack and includes an electromagnetic pressure regulating valve that regulates the pressure of the hydrogen gas to low pressure. The electromagnetic pressure regulating valve includes a housing, and a valve passage connecting a primary port and a secondary port is formed in the housing. A valve body controls an opening degree of the valve passage and is provided in the housing. A high-pressure sealing member and low-pressure sealing member are provided on an outer periphery of the valve body. The high-pressure sealing member and the low-pressure sealing member are provided in this order from one end side of the valve body to the other end side thereof. The electromagnetic pressure regulating valve further includes a housing pressure equalizing passage connecting the secondary port and a buffer chamber formed between the high-pressure sealing member and the low-pressure sealing member.

A valve device for a vehicle may include a pneumatic valve having first and second chambers in which respective pressures thereof are regulated, and a pressure regulation valve coupled to the pneumatic valve, the pressure regulation valve communicating with the first chamber, including a positive pressure side opening and a negative pressure side opening, and selectively opening and closing the positive pressure side opening and the negative pressure side opening to regulate the pressure in the first chamber.

A cap with electromagnetic proportional valve, includes a pilot cap mounted on a valve housing and defining a pilot chamber, and an electromagnetic proportional valve mounted on the pilot cap and adapted to control a pressure of a working fluid supplied to the pilot chamber. The pilot cap includes a supply port into which a working fluid of a fluid pressure supply source is supplied, a discharge port from which the working fluid is discharged to the tank, a primary pressure passage which leads the working fluid at a primary pressure to the electromagnetic proportional valve from the supply port, a secondary pressure passage which leads the working fluid whose pressure is reduced to a secondary pressure by the electromagnetic proportional valve to the pilot chamber, and a drain passage which leads the working fluid discharged from the electromagnetic proportional valve to the discharge port.

A cap with electromagnetic proportional valve, includes a pilot cap mounted on a valve housing and defining a pilot chamber, and an electromagnetic proportional valve mounted on the pilot cap and adapted to control a pressure of a working fluid supplied to the pilot chamber. The pilot cap includes a supply port into which a working fluid of a fluid pressure supply source is supplied, a discharge port from which the working fluid is discharged to the tank, a primary pressure passage which leads the working fluid at a primary pressure to the electromagnetic proportional valve from the supply port, a secondary pressure passage which leads the working fluid whose pressure is reduced to a secondary pressure by the electromagnetic proportional valve to the pilot chamber, and a drain passage which leads the working fluid discharged from the electromagnetic proportional valve to the discharge port.

A redundant valve system to provide a regulated fluid flow includes a housing having an inlet at an inlet end which receives a pressurized fluid, and an outlet at an outlet end which provides the regulated fluid flow, a first piston assembly arranged in the housing having a first cavity, the first piston assembly configured to regulate the fluid flow, and a first fluid connection connected to the first cavity and to a point along the housing at the outlet end. The redundant valve system may further include a second piston assembly arranged in the housing downstream of the first piston assembly having a second cavity, the second cavity having a second fluid connection to a vent, an upstream valve to control the first fluid connection and control a position of the first piston assembly, and a downstream valve to control the second fluid connection and control a position of the second piston assembly.

A redundant valve system to provide a regulated fluid flow includes a housing having an inlet at an inlet end which receives a pressurized fluid, and an outlet at an outlet end which provides the regulated fluid flow, a first piston assembly arranged in the housing having a first cavity, the first piston assembly configured to regulate the fluid flow, and a first fluid connection connected to the first cavity and to a point along the housing at the outlet end. The redundant valve system may further include a second piston assembly arranged in the housing downstream of the first piston assembly having a second cavity, the second cavity having a second fluid connection to a vent, an upstream valve to control the first fluid connection and control a position of the first piston assembly, and a downstream valve to control the second fluid connection and control a position of the second piston assembly.