A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

An electronic device includes a housing and an internal pressure regulator valve. The internal pressure regulator valve includes a valve body of flexible material. The valve body has a valve hole and a hollow. The hollow is continuous with the valve hole and opens into the housing. Through the valve hole, gas is released to outside.

Provided is a centrifuge cassette assembly (800) for separating a fluid and related method of manufacture. The cassette assembly includes a first chamber, a second chamber (308), a fluidic channel (808) creating a fluid connection between the first chamber and the second chamber, at least one molded insertion valve (600, 700) configured to control the flow of fluid in the fluidic channel and a heating element (802) for actuating the at least one molded valve. Further provided are Normally Open Valves (NOVs) (700) and Normally Closed Valves (NCVs) (600) which are capable of insertion into, and which control the fluid flow of, the centrifuge cassette assembly.

Modular microvalves for microfluidic devices and methods of using the same are disclosed. Further, a microfluidic system is provided that may include the presently disclosed modular microvalves installed in a microfluidic device. Further, the microfluidic system may include valve actuators in relation to the presently disclosed modular microvalves. Further, the presently disclosed modular microvalves may be manufactured separately and independently of the manufacturing process of any microfluidic device and/or system. Non-limiting examples of the presently-disclosed modular microvalves may include but are not limited to, modular pinch valves, modular rocker valves, modular slider valves, modular rotary valves, and the like.

Electrochemical actuators including a sealed electrolytic chamber with two or more electrodes disposed therein and associated reservoirs are described. In some embodiments, the electrochemical actuators include one or more rigid structures that are overmolded onto one or more electrodes to form the electrolytic chambers. In some embodiments, multiple rigid structures that are overmolded onto two or separate electrodes may be connected to form one or more electrolytic chambers with a desired configuration of electrodes contained therein. Manufacturing methods and structures related to the formation of an array of electrochemical actuators are also described.