A modular cassette is provided for separating a composite fluid into at least two component parts thereof during centrifugation. The modular cassette includes: a housing defining a fluid inlet, a fluid outlet, and a chamber for fluid separation; a fluidic channel configured to provide fluid communication between at least two components of the modular cassette; a heat expanding valve including: a flow pathway including undulations configured to facilitate closing of the fluidic channel, wherein the heat expanding valve occludes one or more of the undulations of the flow pathway to close the fluidic channel; and a heating element configured to actuate the heat expanding valve.

A microfluidic valve may include a firing chamber having an orifice, a first portion of a liquid conduit connected to the firing chamber at a first port, a second portion of the liquid conduit connected to the firing chamber at a second port and a thermal resistor. The thermal resistor is to form a bubble within the firing chamber to expel liquid from the firing chamber through the orifice such that a first meniscus forms across the first port and a second meniscus forms across the second port to interrupt liquid flow between the first portion and the second portion of the liquid conduit.

A fluid check-valve for venting gas from a fluidic system comprises a retention body defining a fluid aperture having an upstream side and a downstream side, a hydrophilic porous material held by the retention body and disposed to cover the fluid aperture, and a hydrophobic porous material held by the retention body and disposed to cover the fluid aperture and adjacent the hydrophilic porous material. One face of the hydrophilic porous material is in fluid communication with the upstream side of the aperture, and one face of the hydrophobic porous material is in fluid communication with the downstream side of the aperture. The hydrophilic porous material is configured to retain liquid from the upstream side to hinder passage of gas from the downstream side to the upstream side, and the hydrophobic porous material is configured to hinder passage of liquid from the upstream side to the downstream side.

The microfluidic devices and systems disclosed herein reduce sample loss and help decrease sample processing bottlenecks for applications such as next generation sequencing (NGS). The microfluidic devices include a plurality of reaction modules. Each reaction module may comprise one or more reaction circuits. Each reaction circuit may comprise a single reaction flow channel with each reaction circuit connected by a bridge flow channel. Alternatively, each reaction circuit may comprise two or more reaction flow channels connected by two or more bridge flow channels. The combination of any two bridge flow channels and a portion of the two or more reaction flow channels between the any two bridge flow channels defining may define the reaction circuit. The reaction module may be arranged as nodes connected by bridge flow channels or each reaction module may be arranged in a parallel fashion on the microfluidic device.

A micro fluid actuator includes a first substrate, a chamber layer, a vibration layer, a first metal layer, a piezoelectric actuation layer, a second metal layer, a second substrate, an inlet layer, a resonance layer and an aperture array plate. The first substrate includes a plurality of first outflow apertures and a plurality of second outflow apertures. The chamber layer includes a storage chamber. The second metal layer includes an upper electrode pad and a lower electrode pad. While driving power having different phase charges is provided to the upper electrode pad and the lower electrode pad to drive and control the vibration layer to displace in a reciprocating manner, the fluid is inhaled from the exterior through the inlet layer, converged to the storage chamber, compressed and pushes out the aperture array plate, and then is discharged out from the micro fluid actuator to achieve fluid transportation.

A valve includes a first plate, a second plate, a spacer disposed between the first plate and the second plate, and a flap movably disposed between the first plate and the second plate. The first plate includes a plurality of first apertures extending through said first plate and the second plate includes a plurality of second apertures extending through said second plate. The second apertures are substantially offset from the first apertures. The spacer forms a cavity between the first plate and the second plate and is in fluid communication with the first apertures and the second apertures. The flap has apertures substantially offset from the first apertures and substantially aligned with the second apertures, and the flap is operable to be motivated between said first and second plates in response to a change in direction of the differential pressure of the fluid across the valve.

A fluid control apparatus includes a piezoelectric pump and valve. The valve includes a second valve housing, second seal member, diaphragm, first seal member, and first valve housing and has a structure in which they are laminated in sequence. The first valve housing includes a second vent and third vent, has a valve seat, and includes six cavities. The second valve housing has a first vent and first vent and includes a valve seat and six first protrusions. The second valve housing further includes six second protrusions nearer the outer edges than the six first protrusions, as seen in the x-axis direction in plan view.

Methods and systems are provided for a microfluidic cartridge including a high performance actuator useful for analyte detection, labeling and analysis. Microfluidic processing systems are to carry out chemical or biochemical reactions, or sequences of reactions, with small volumes (typically between 1 microliter and 10 milliliters) of reactants and products. A microfluidic processing system can comprise a network of tubes interfaced with discrete components such as valves and sensors, or an integrated device made of plastic, glass, metal, or other materials, or a combination of materials, with components such as valves and sensors built into the device and connected by flow passageways formed in the material.

A valve module includes a semiconductor body, cavities in the semiconductor body separated from each other by a distance, a cantilever structure suspended over each cavity to enable at least partial closing of the cavity, and a piezoelectric actuator for each cantilever structure. The piezoelectric actuator is configured for use to cause a positive bending of the respective cantilever structure and so modulate a rate of air flow through the valve module.

A device for biofluid processing and analysis includes a microfluidic module including multiple stacked layers, each layer of the stacked layers defines a respective conduit, and conduits of the stacked layers are interconnected to provide a flow path for a biofluid.