A method of generating a pressurized, sub-cooled mixed-phase cryogen is disclosed, including providing a cryogenic system including a reservoir containing a liquid cryogen; and a heat exchange coil immersed in the liquid cryogen, the heat exchange coil having an input end and an output end not immersed in the liquid cryogen; introducing a pressurized gas cryogen to the input end of the heat exchange coil; cooling the pressurized gas cryogen within the heat exchange coil; and collecting the pressurized gas cryogen at an output end of the heat exchange coil.

Provided is a manifold attachment assembly for attachment to a cryogenic cylinder. The assembly includes a knuckle configured to be attached to the cryogenic cylinder and a manifold configured to be attached to the knuckle. The manifold has an upper surface, a lower surface configured to abut an upper surface of the knuckle, a plurality of gas passages extending through the manifold for aligning with a corresponding one of a plurality of gas passages in the knuckle, and a plurality of valve seats in the upper surface each in communication with one of the plurality of gas passages in the manifold. The assembly also includes a plurality of valves respectively secured to the manifold in one of the plurality of valve seats. The attachment assembly provides a modular assembly that reduces refurbishment and requalification time, reduces assembly labor, and simplifies servicing.

Dual level sensing, vacuum monitoring, smart supply cylinders, smart oxygen monitors, and real time data collection functionality are integrated with a cryogenic storage system to improve sample safety and user safety. The dual level sensing system includes two liquid nitrogen level sensors installed into a cryogenic freezer that send redundant level information to a controller. Vacuum monitoring is performed via a gauge, installed on a vacuum port, that communicates data to the controller. The controller further receives level information from supply cylinders and oxygen levels from one or more oxygen monitors. The aforementioned data sources can be integrated into control decisions executed by the controller. In addition, all operational data of the cryogenic storage system is communicated to a cloud-based platform to provide real-time status monitoring, trend analysis, and alarm notifications.

Systems and methods for monitoring environmental variables associated with temperature-sensitive products during shipping may include sensors attached to or inside a container being shipped and a remote processor receiving data provided by the sensors. For instance, temperature and orientation may be sensed over time and a status associated with a temperature-sensitive product may be determined based on the temperature and/or orientation and based on changes in the temperature and/or orientation over time. In this manner, potentially deleterious temperature and/or orientation excursions may be predicted and monitored.

The present invention provides a sterilized-liquefied gas producing apparatus including: a liquefied gas reservoir; a source-gas supplier that supplies a source gas to the liquefied gas reservoir; a cooler that cools down an inside of the liquefied gas reservoir to liquefy the source gas; a supply pipe that connects the source-gas supplier and the liquefied gas reservoir; a sterilization filter provided at the supply pipe; a sterilizer that heats and sterilizes a sterilization region by high-temperature saturated water vapor, the sterilization region being located further downstream than the sterilization filter; and a dryer that removes moisture generated by the heat sterilization to dry the sterilization region.

A cryogenic system as well as a method of generating a pressurized, sub-cooled mixed-phase cryogen and a method of delivering such a cryogen to a cryoprobe are disclosed. In an embodiment, the cryogenic system includes a reservoir containing a liquid cryogen and a sub-cooling coil immersed in the liquid cryogen. The cryogen is supplied to the sub-cooling coil and is cooled under pressure to produce a pressurized mixed phase cryogen within the sub-cooling coil. This pressurized mixed phase cryogen is provided via supply line to a cryo-device for use.

A cryogenic system as well as a method of generating a pressurized, sub-cooled mixed-phase cryogen and a method of delivering such a cryogen to a cryoprobe are disclosed. In an embodiment, the cryogenic system includes a reservoir containing a liquid cryogen and a sub-cooling coil immersed in the liquid cryogen. The cryogen is supplied to the sub-cooling coil and is cooled under pressure to produce a pressurized mixed phase cryogen within the sub-cooling coil. This pressurized mixed phase cryogen is provided via supply line to a cryo-device for use.

A dewar for storing a cryogenic fluid features an inner vessel configured to store the cryogenic fluid and an outer vessel having an outer upper head and an outer lower head. The outer upper and lower heads are joined so as to define an interior chamber of the dewar. The inner vessel is positioned within the interior chamber of the outer vessel so that an insulation space, which is evacuated of air, is defined between the inner and outer vessels. A neck extends between the inner vessel and a central region of the outer upper head. The outer upper head and neck are configured so that the central region permanently deforms without breaking the neck when excessive shock loads are applied to the dewar.

A tube-array-type liquid nitrogen container includes a container body having a mouth; a tube array component received in the container body; and a top cap sealing the mouth from above. The top cap is rotatable in the mouth. The tube array component is composed of a plurality of holding tubes. The holding tube is opened at one end thereof, wherein the opening thereof faces the top cap. The top cap has at least one tube access passing therethrough. Each tube access is atop covered by a tube access cover. The tube-array-type liquid nitrogen container uses a tube-array component composed of the a plurality of holding tubes to store the freezing tubes, and is cooperated with the rotatable top cap and an external robotic arm, thereby improving space utilization and thermal insulation, effectively ensuring safety of the freezing tubes, and facilitating automatic storage of freezing tubes.

A doser for dispensing a cryogenic fluid includes a doser body configured to receive the cryogenic fluid. The dosing arm has a proximal end and a distal end and a central passage extending between the proximal and distal ends. Furthermore, the dosing arm is configured to receive cryogenic fluid from the doser body. A bayonet connection removably connects the proximal end of the dosing arm to the doser body. A dosing head is mounted to the distal end of the dosing arm and is configured to receive cryogenic fluid from the central passage of the dosing arm and to dispense the cryogenic fluid.