This invention describes a design for a multiwell plate that contains integrated pumps that are used to stir each well of the plate. The device employs microfluidic logic technology to drive each peristaltic pump. This enables the plates to run autonomously, requiring only a static vacuum supply for power. The devices are entirely constructed out of low-cost polymers, with no electronics, and yet contains simple digital logic circuits to control the pumps. A stack of these plates may be run continuously in a standard cell culture incubator, allowing high-throughput culture of organoids.

A fluid delivery device includes: a first chamber configured to receive fluid from a fluid reservoir; a second chamber configured generate a vacuum therein to apply pressure to the fluid in the first chamber to enable the fluid in the first chamber to be output from the fluid delivery device; and a flow control member configured to allow the fluid in the first chamber to flow through the flow control member into the fluid reservoir at a predetermined flow rate to decrease the vacuum in the second chamber.

In order to make the two pump units which are generally present in an emptying device for a storage container and which feed in alternating fashion as compact as possible on the one hand and subject to as little wear as possible on the other hand, both pump units are preferably equipped with a feed pump in the form of a diaphragm pump, which is driven by a hydraulically operated working cylinder unit.

A sterile liquid pump, having replaceable single use components, with a first and second chamber, and a gas valve assembly to selectively communicate gas pressure and vacuum with the chambers, and a resilient tubing liquid manifold loop with a sequence of four ports located within a manifold receiver that supports four pinch actuators aligned to engage and selectively pinch-off flow through the manifold between adjacent pairs ports, and, a controller that operates the valve assembly to alternatingly couple pressure and vacuum to the pump chambers, and that also operates to alternatingly actuate pairs of the pinch actuators to sequentially pump fluid from pump chambers under gas pressure, and through an opposing pair of ports in the resilient tubing manifold.

Microfluidic oscillator circuits and pumps for microfluidic devices are provided. The microfluidic pump may include a plurality of fluid valves and a microfluidic oscillator circuit having an oscillation frequency. The fluid valves may be configured to move fluids. Each fluid valve may be connected to a node of the microfluidic oscillator circuit. The pumps may be driven by the oscillator circuits such that fluid movement is accomplished entirely by circuits on a microfluidic chip, without the need for off-chip controls.

A fluid delivery device includes: a first chamber configured to receive fluid from a fluid reservoir; a second chamber configured generate a vacuum therein to apply pressure to the fluid in the first chamber to enable the fluid in the first chamber to be output from the fluid delivery device; and a flow control member configured to allow the fluid in the first chamber to flow through the flow control member into the fluid reservoir at a predetermined flow rate to decrease the vacuum in the second chamber.