The present disclosure provides guided microbial remodeling (GMR) methods for the rational improvement of plant-associated microbes to perform plant-beneficial functions. The GMR methods described herein allow for non-intergeneric genetic optimization of key regulatory networks within the microbes, which improve plant-beneficial functions over wild-type microbes but don't have the risks associated with transgenic approaches (e.g., unpredictable gene function, public and regulatory concerns, etc.). The present disclosure also provides remodeled microbes and compositions thereof. The utilization of remodeled microbes and compositions thereof will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived fertilizers.

The disclosure relates to the field of agricultural biotechnologies, and an application of a Zm00001d030087 gene in regulating protein content of maize kernels is provided. A sequence of the Zm00001d030087 gene is as shown in SEQ ID NO: 1. The gene is identified as a functional gene for regulating kernel protein content. By the gene, the protein content of the maize kernels can be regulated, and a basis for breeding maize varieties with high protein content can be provided.

This invention provides a Brassica oleracea plant with high S-methylmethionine content. This invention relates to a Brassica oleracea plant with higher S-methylmethionine content than that in a conventional Brassica oleracea plant. Specifically, this invention relates to a Brassica oleracea plant with the S-methylmethionine content of 48 mg/100 g fresh weight or more.

The present disclosure provides methods and compositions for producing, detecting, and selecting soybean plants and seeds comprising at least one high protein CCT (CONSTANS, CO-like and TOC1) domain containing variant allele and introgressing the high protein CCT variant allele into soybean plants. The present disclosure also provides methods and compositions for producing, detecting, and selecting soybean plants producing seeds having a high protein content including breeding methods for introgressing high protein alleles into soybean plants using marker assisted selection using markers linked to or associated with high protein CCT in soybean.