The present invention provides in a first aspect a mouse in which the (lambda) light chain locus has been functionally silenced. In one embodiment, the mouse light chain locus was functional silenced by deletion of gene segments coding for the light chain locus. In a further aspect, a mouse containing functionally silenced and (kappa) L chain loci was produced. The invention is useful for the production of antibodies, for example heterologous antibodies, including heavy chain only antibodies.

The invention in some aspects relates to isolated nucleic acids, compositions, and kits useful for identifying adeno-associated viruses in cells. In some aspects, the invention provides kits and methods for producing somatic transgenic animal models using recombinant AAV (rAAV) to an animal having at least one transgene that expresses a small interfering nucleic acid or at least one binding site for a miRNA.

The present invention relates to the specific inhibition of gene expression in mammals by bringing the target gene into contact with double stranded RNA (dsRNA).

This invention entails a method to make a Tra-2 RNAi kernel sequence, and uses for this kernel sequence. The method includes the steps of amplifying an RNA recognition motive (RRM) DNA sequence from a Culcinae mosquito species, to obtain a first RRM DNA sequence of 240 base pairs; then reversely connecting the first RRM DNA sequence with a second RRM DNA sequence via an intron or a linker DNA sequence, in such a way that the second RRM DNA sequence forms an inverted sequence to the first RRM DNA sequence. Transcription of the two DNA sequences produces single strands of mRNA that can bind together to form a double strand hairpin mRNA structure. Other steps include inserting a tetracycline repressible transactivator, and an insect spermatogenesis promoter.

Certain embodiments are directed kits comprising components for producing a mammalian cancer model. In certain aspects the components are expression vectors. In certain embodiments one or more expression vector is engineered to express a Kras.sup.G12D polypeptide, a p53 transcriptional suppressor, SMAD4 transcriptional suppressor, p16/CDKN2A transcriptional suppressor. In certain aspects the transcriptional suppressor is a short hairpin RNA (shRNA) or other nucleic acid used for RNA interference.

Provided herein are methods of producing trabecular bone material, such as trabecular bone or trabecular bone matrix and cells useful in preparing the trabecular bone material.

Disclosed are compositions, methods, and kits for modifying DNA within cells as well as compositions and methods for modifying gene expression in a cell. In particular, the invention generally relates to compositions, methods, and kits for DNA editing using single-stranded DNA. Compositions and methods for modifying gene expression using artificial microRNAs (amiRNA) are also contemplated.

A bovine animal or offspring thereof or a bovine cell or gene comprising a modified chromosomal sequence in at least one allele of a gene encoding an Alpha-actinin-3 protein, wherein said modified chromosomal sequence partially or completely impairs the ability of said animal/cell/gene to encode said protein. The bovine retains all previous functionality, but is distinguished by a reduction in the number and cross-sectional area of Type II muscle fibers, and an increase in the proportion of Type I muscle fibers, resulting in improved feedlot efficiency, improved meat quality, and reduced emissions output.

Disclosed are compositions, methods, and kits for modifying DNA within cells as well as compositions and methods for modifying gene expression in a cell. In particular, the invention generally relates to compositions, methods, and kits for DNA editing using single-stranded DNA. Compositions and methods for modifying gene expression using artificial microRNAs (amiRNA) are also contemplated.

Methods and compositions using a CRISPR-Cas Type IIIA resulting in RNA gene knockdown and knockout in an animal.