Methods of using split G-quadruplexes associated with functional tags for associating said tags to target nucleic acids. Methods include use of split G-quadruplexes associated with detection tags for the detection of target nucleic acids, and use of split G-quadruplexes associated with capture tags for detection or capture of target nucleic acids.

A unitized valve body for use in an automatic transmission includes a plurality of first hydraulic passages, a second hydraulic passage and a plurality of orifices. The second hydraulic passage extending through the unitized valve body and configured to be in fluid communication with a plurality of valve bores. Each orifice disposed within the unitized valve body and fluidly connecting the second hydraulic passage to a respective first hydraulic passage of the plurality of first hydraulic passages.

A unitized valve body for use in an automatic transmission includes a plurality of first hydraulic passages, a second hydraulic passage and a plurality of orifices. The second hydraulic passage extending through the unitized valve body and configured to be in fluid communication with a plurality of valve bores. Each orifice disposed within the unitized valve body and fluidly connecting the second hydraulic passage to a respective first hydraulic passage of the plurality of first hydraulic passages.

A unitized valve body for use in an automatic transmission includes a valve bore and an annulus. The valve bore is configured to receive a valve. The annulus is in fluid communication with the valve bore. The annulus defines an outer portion having an outer diameter and an innermost portion located at an interface between the valve bore and the valve. The outer portion having a first axial length and the innermost portion having a second axial length. The first axial length is greater than the second axial length.

A valve device for an automatic transmission for a vehicle, preferably for a motor or utility vehicle, is provided. According to the invention, the valve device is designed such that the valve device can achieve the establishment and separation of a fluid connection between an operating pressure side and an actuator pressure side, each in a state operated without pressure. Further, an automatic transmission having such a valve device and a corresponding method for controlling such a valve device are provided.

An automatic transmission where the control portion controls the adjustment solenoid valve so that the circulation hydraulic pressure equals to a second circulation hydraulic pressure higher than the first circulation hydraulic pressure when the rotational speed difference between the output rotational speed of the fluid transmission device and the rotational speed of the driving source is more than the predetermined rotational speed.

An assembly for actuating a double-acting shift cylinder of a shift assembly of an automatic transmission of a motor vehicle includes a hydraulic fluid reservoir having a motor-driven hydraulic pump and two control valves that are each connected to a pressure supply line of the hydraulic pump and to a return line to the hydraulic fluid reservoir. The shift cylinder has two pressure chambers that are each connected to one of the control valves. The control valves each connect one pressure chamber of the shift cylinder to the pressure supply line of the hydraulic pump or to the return line to the hydraulic fluid reservoir. The actuating assembly includes at least one pressure-limiting valve that connects the pressure supply line to the return line if a hydraulic over-pressure is present. The control valves can be 3/2-directional valves having integrated pressure-limiting valves.

A fast valve actuation system for an automatic vehicle transmission includes a pair of spring-biased shift valves. Solenoids control the application of pressurized hydraulic fluid to the head of each of the shift valves. Each shift valve has at least one port that is coupled to a fluid chamber of a torque transferring mechanism of an automatic transmission. The position of each of the shift valves determines whether its ports are connected with fluid pressure. Fluid passages connect the head of each shift valve to the spring pocket of the other shift valve.

An oil pressure control device controls a supply pressure of oil supplied to an oil pressure chamber of a friction element having a first engagement board and a second engagement board engaged with or disengaged from each other between a non-engagement state and a non-sliding engagement state via a sliding engagement state. The oil pressure control device includes: an electromagnetic valve that controls the supply pressure using a spool which reciprocates according to an energizing amount; and a control part that defines a first oscillatory wave part in which the energizing amount is controlled by making a first waveform with a first frequency to superimpose on a second waveform with a second frequency higher than the first frequency during a first period while the sliding engagement state and an instruction value of the supply pressure are kept constant.

A system for locking and releasing a shifting position of a shifting element that is hydraulically actuatable by an actuating pressure is provided. The shifting element is arranged coaxially with a transmission shaft. The system includes a stop valve. The stop valve is subjectable to a control pressure that is separate from the actuating pressure of the shifting element. The stop valve is arranged radially inside the shifting element in the transmission housing.