A transmission system has a housing including a wall and a gear support extending from the wall. The gear support has an outer surface and an inner surface defining a passage. The outer surface includes a seal receiving portion and a seal support axially spaced from the seal receiving portion. A seal is positioned about the gear support at the seal receiving portion. A sleeve is arranged on the outer surface of the gear support between the wall and the seal and a pump drive gear is mounted on the gear support and is supported by the sleeve. The pump drive gear includes an outer toothed surface, an inner surface, and a bushing arranged on the inner surface, the bushing extending about the sleeve.

A transmission system has a housing including a wall and a gear support extending from the wall. The gear support has an outer surface and an inner surface defining a passage. The outer surface includes a seal receiving portion and a seal support axially spaced from the seal receiving portion. A seal is positioned about the gear support at the seal receiving portion. A sleeve is arranged on the outer surface of the gear support between the wall and the seal and a pump drive gear is mounted on the gear support and is supported by the sleeve. The pump drive gear includes an outer toothed surface, an inner surface, and a bushing arranged on the inner surface, the bushing extending about the sleeve.

A controller drives a shifting actuator to move a first engagement member from an original position of a first engagement portion in one direction along a relative motion path and acquires first position information representing a first position, the first position being a position where the first engagement portion moved in the one direction contacts a second engagement portion. The controller drives the shifting actuator to move the first engagement member from the original position in the other direction along the relative motion path and acquires second position information representing a second position where the first engagement portion moved in the other direction contacts the second engagement portion. The controller calculates a center position of the first engagement portion on the relative motion path from the acquired first position information and the acquired second position information, compares the calculated center position to the original position.

A transmission includes an input shaft coupled to a prime mover, a countershaft, main shaft, and an output shaft, with gears between the countershaft and the main shaft. A shift actuator selectively couples the input shaft to the main shaft by rotatably coupling gears between the countershaft and the main shaft. The shift actuator is mounted on an exterior wall of a housing including the countershaft and the main shaft. An integrated actuator housing is operationally coupled to the shift actuator and a linear clutch actuator. The linear clutch actuator is a self-adjusting actuator, and the transmission includes a self-adjusting clutch.

A transmission includes an input shaft coupled to a prime mover, a countershaft, main shaft, and an output shaft, with gears between the countershaft and the main shaft. A shift actuator selectively couples the input shaft to the main shaft by rotatably coupling gears between the countershaft and the main shaft. The shift actuator is mounted on an exterior wall of a housing including the countershaft and the main shaft. An integrated actuator housing is operationally coupled to the shift actuator and a linear clutch actuator. The linear clutch actuator is a self-adjusting actuator, and the transmission includes a self-adjusting clutch.

A tandem drive axle mechanical shift assembly including a first shift rail assembly having a first shift rail actuated via a first pneumatic actuator, and a shift fork having a first end coupled with the first shift rail and a second end coupled with an engagement selector. A second shift rail assembly having a second shift rail actuated via a second pneumatic actuator, and a second shift fork having a first end coupled with the second shift rail and a second end coupled with an inter-axle differential lock-up clutch. First and second primary valves in fluid communication with a reservoir, and a secondary valve in fluid communication with the reservoir and in selective fluid communication with the second pneumatic actuator. A first actuation valve operated by the first shift rail and in selective fluid communication with the first and second primary valves, and the first and second pneumatic actuators.

A tandem drive axle mechanical shift assembly including a first shift rail assembly having a first shift rail actuated via a first pneumatic actuator, and a shift fork having a first end coupled with the first shift rail and a second end coupled with an engagement selector. A second shift rail assembly having a second shift rail actuated via a second pneumatic actuator, and a second shift fork having a first end coupled with the second shift rail and a second end coupled with an inter-axle differential lock-up clutch. First and second primary valves in fluid communication with a reservoir, and a secondary valve in fluid communication with the reservoir and in selective fluid communication with the second pneumatic actuator. A first actuation valve operated by the first shift rail and in selective fluid communication with the first and second primary valves, and the first and second pneumatic actuators.

Methods of using split G-quadruplexes associated with functional tags for associating said tags to target nucleic acids. Methods include use of split G-quadruplexes associated with detection tags for the detection of target nucleic acids, and use of split G-quadruplexes associated with capture tags for detection or capture of target nucleic acids.

A transmission system has a housing including a wall and a gear support extending from the wall. The gear support has an outer surface and an inner surface defining a passage. The outer surface includes a seal receiving portion and a seal support axially spaced from the seal receiving portion. A seal is positioned about the gear support at the seal receiving portion. A sleeve is arranged on the outer surface of the gear support between the wall and the seal and a pump drive gear is mounted on the gear support and is supported by the sleeve. The pump drive gear includes an outer toothed surface, an inner surface, and a bushing arranged on the inner surface, the bushing extending about the sleeve.

A transmission system has a housing including a wall and a gear support extending from the wall. The gear support has an outer surface and an inner surface defining a passage. The outer surface includes a seal receiving portion and a seal support axially spaced from the seal receiving portion. A seal is positioned about the gear support at the seal receiving portion. A sleeve is arranged on the outer surface of the gear support between the wall and the seal and a pump drive gear is mounted on the gear support and is supported by the sleeve. The pump drive gear includes an outer toothed surface, an inner surface, and a bushing arranged on the inner surface, the bushing extending about the sleeve.