A live tissue storage solution is formed of an aqueous culture medium including additives, inorganic salts and nutrients. The additives preferably include amino acids including but not limited to L-Arg, L-Cys, Gly, L-His, L-Ile, L-Lys, L-Leu, L-Met, L-Phe, L-Ser, L-Try, L-Try, and L-Val. The inorganic salts preferably include calcium chloride, sodium chloride, magnesium sulphate, sodium bicarbonate, sodium phosphate. The nutrients preferably include glucose, choline, folic acid, nicinamide, riboflavin, which are known to maintain cell culture. Examples include Dulbecco's Modified Eagle Medium DMEM or Minimal Essential Medium MEM with or without serum. The culture medium contains additives to reduce, inhibit, or mitigate lipid oxidation, lipotoxicy, lipid-induced responses, and lipid inflammation.

Methods and apparatus use low temperature and elevated pressure to depress the freezing and melting temperature of water and aqueous solutions, to induce suspended animation in materials including but not limited to biological material, soluble molecules, organic and inorganic compounds. Disposing such materials in a pressure vessel and increasing the pressure to about 210 MPa depresses the freezing and melting temperature of water, biological matter, and materials in aqueous solution, to about −22° C. Storage at low temperature under high pressure suspends metabolic activity and induces cryostasis. The methods and apparatus may be used for cryo-banking biological materials that cannot be frozen or vitrified, or otherwise preserved, including, but not limited to, cells, tissues, human organs for transplantation, and entire organisms.

The present invention aims to provide a device for cryopreservation providing excellent working efficiency in operations for freezing and thawing cells or tissues. Provided is a device for cryopreservation off a cell or tissue including at least: a body including a strip-shaped tip; and a cap covering the tip of the body, the tip including at least a deposition part comprising a preservation solution absorber, the tip having a length in a minor axis direction of not shorter than 70% of an inner diameter of the cap, the cap including an inner cavity having a length in a major axis direction of not longer than 200% of a length in a major axis direction of the tip.

Organ and tissue preservation solutions having improved stability are disclosed. The solutions are comprised of two separate solutions. The first solution, Solution A, is comprised of a balanced salt solution that is stable in solution at a pH of 7.0 or above. A second solution, Solution B, is comprised of an aqueous solution containing L-glutathione and/or cysteinylglycine, a sugar such as D-glucose, L-Arginine, a reducing agent such as ascorbic acid and water at a pH of below 7.0, preferably from about 3.0 to 5.0. The two Solutions are then mixed together at the point of use and the pH adjusted to about 7.3 resulting in the organ and tissue preservation solution having improved stability. Preferably, solution A has a pH of about 7.6 and solution B has a pH of about 5.0. The present invention is also comprised of kits 20 that contain the two Solutions in two separate containers 22, 24. In an alternate embodiment, the sugar can be in Solution A.

Blood and platelet storage and rejuvenating compositions that include triciribine, triciribine metabolites, triciribine analogs, and mixtures of the same are disclosed. Such compositions can be useful in methods for treating (e.g., storing and rejuvenating) red blood cells and platelets.

Provided herein are constructs of micro-aggregate multicellular, minimally polarized grafts containing Leucine-rich repeat-containing G-protein coupled Receptor (LGR) expressing cells for wound therapy applications, tissue engineering, cell therapy applications, regenerative medicine applications, medical/therapeutic applications, tissue healing applications, immune therapy applications, and tissue transplant therapy applications which preferably are associated with a delivery vector/substrate/support/scaffold for direct application.

The present invention provides a decellularized collagenous tissue and a method for processing an artificial heart valve containing the decellularized collagenous tissue. The method comprises immersing the decellularized collagenous tissue in a preservation liquid containing an ionic liquid until the size of the decellularized collagenous tissue remains stable in a non-liquid environment. The present invention also provides a preservation method, comprising immersing the decellularized collagenous tissue or the artificial heart valve containing decellularized collagenous tissue in the preservation liquid containing ionic liquid until the size of the collagen tissue remains stable in the non-liquid environment. The present invention also provides a decellularized collagenous tissue and an artificial heart valve containing the decellularized collagenous tissue processed by the method described above. The method can effectively avoid a biological material damage caused by being in a non-liquid environment and is beneficial to implement a subsequent processing and sterilization for the biological material.

The present disclosure relates to Oxygen Reduction Disposable kits (ORDKit), devices and methods for the improved preservation of whole blood and blood components. The improved devices and methods for the collection of blood and blood components provide for whole blood and blood components having reduced levels of oxygen. The devices and methods provide for the rapid preparation of deoxygenated blood and blood components for storage that improves the overall quality of the transfused blood and improves health outcomes in patients.

Provided herein are methods of preserving PBMCs, including lyophilizing PBMCs, and compositions thereof. In some embodiments, such compositions include freeze-dried or rehydrated PBMCs in an aqueous mixture comprising a cryoprotectant, a lyoprotectant, and a buffer. In some embodiments, such compositions further include a PBMC cell culture media. Furthermore, provided herein are methods for preparing freeze-dried PBMCs and for preparing rehydrated PBMCs, as well as methods for administering freeze-dried PBMCs.

The present inventive concept provides non-toxic compositions including ethanol, a polymer, and a polar aprotic solvent and methods of using the same in the field of preservation of plant, human and non-human animal tissue and anatomic pathology disciplines (e.g., surgical pathology, histopathology, cytopathology, forensic pathology). In particular, the compositions provide a safer alternative to aldehyde-based compositions.