The present invention is directed to a cryogenic cooler that may include a cold finger coupled to a cooling source, an insulating vessel having an inner wall, an outer wall and an interior area formed within the inner wall, where the cold finger may be positioned at least partially within the interior area. The cryogenic cooler may also have a fitting positioned on the outer wall and configured for coupling to a gas source, and a passageway formed between the coupling and the inner wall and configured to allow the transfer of a gas from the gas source to the interior area. The cryogenic cooler may also have an opening is formed in the interior area so as to allow the transfer of the gas around the cold finger and out of the interior area.

Microfluidic platforms for forming and culturing perfusable hydrogel vascularized tissues typically include one or more culture chambers. Each culture chamber includes at least two openings overlaid over a gel channel. The gel channel typically includes at least two tissue zones and a trapping or insertion portion positioned between the tissue zones. The trapping or insertion portion permits vascular networks to develop between the two tissue zones containing vascularized tissues and/or vascularized tissue masses. The vascularized tissue masses in the tissue zones of the gel channel are connected indirectly, via the vascular network of the trapping portion. Also described are methods of forming and culturing perfusable vascularized tissue masses directly or indirectly interconnected via vascularized networks.

The present disclosure describes systems, devices, compositions, and methods for live tissue preservation.

This application provides methods, compositions, and kits for use blood group determination and the preparation of improved red blood cell containing reagents for use in blood typing of blood prior to its use in transfusion medicine.

The present invention provides a method and apparatus for tissue, such as an allograft, storage and preservation for extended periods of time at room temperature in a sterile tissue culture chamber. The invention further provides a process for maintaining the sterility of tissue using the apparatus as described.

Provided herein are systems, methods, and kits for controlling and monitoring environmental conditions of a semen sample during transport. The technologies disclosed herein allow for at-home male fertility sample collection and subsequent transport to a laboratory to undergo fertility testing. One or more environmental conditions can be tracked during transport which provide the data used to determine whether a sample needs to be resubmitted or if the conditions during transport were such that a false test result is unlikely.

Provided herein are constructs of micro-aggregate multicellular, minimally polarized grafts containing Leucine-rich repeat-containing G-protein coupled Receptor (LGR) expressing cells for wound therapy applications, tissue engineering, cell therapy applications, regenerative medicine applications, medical/therapeutic applications, tissue healing applications, immune therapy applications, and tissue transplant therapy applications which preferably are associated with a delivery vector/substrate/support/scaffold for direct application.

Presented herein are ready to administer (RTA) retinal pigment epithelium (RPE) cell therapy compositions for the treatment of retinal degenerative diseases and injuries. A method of formulating human RPE cells for administration to a subject directly after thawing and of formulating RPE cell therapy compositions for cryopreservation and administration of the cryopreserved composition to a subject subsequent to thawing are also presented. In another aspect, the RTA composition may be formulated as a thaw and inject (TAI) composition, whereby the composition is administered by injection subsequent to thawing.

The present disclosure provides systems, methods, devices, and kits for analysis of vaginal biological samples. A device for the analysis of vaginal biological samples can include a sample collector, an extractor, and an assay cartridge. A method for the analysis of vaginal biological samples can include detecting the presence or absence of a pathology, a disease, an immune disorder, a reproductive disorder of a subject. The method may further comprise preserving, storing, or transporting the vaginal biological samples. A kit for the analysis of vaginal biological samples can include probe, reagents and instructions for detecting a nucleic acid in the vaginal biological samples.

Methods, systems, and devices preserve donor organs by alternating between static cold storage and ex vivo organ perfusion. Preserving a donor organ includes refrigerating a donor organ to form a once-refrigerated donor organ, perfusing the once-refrigerated donor organ to form a once-perfused donor organ, and refrigerating the once-perfused donor organ to form a preserved, twice-refrigerated donor organ for transplantation.