Methods, additive kits and storage compositions for decreasing the deleterious effects of storage on pRBCs, for improving the aging process of stored pRBCs, and for preventing or ameliorating patient comorbidities following the transfusion or infusion of stored blood based on inhibiting acid sphingomyelinase during storage are provided.

Cells or tissues are preserved in an unfrozen state in the liquid composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion. The acidic polysaccharide may be alginic acid. The liquid composition may further contain a metal cation such as calcium ion and the like.

Pharmaceutical compositions comprising stabilized self-assembling amphiphilic molecular structure (SAMS) having average diameters of 120 nm or less with a low zeta potential of less than 30 mV to be used for Total Parenteral Nutrition (TPN) or Total Parenteral Alimentation (TPA), increase blood pressure, renal dialysis, transport and release hydrophobic gases, exchange transfusion to replace blood, extracorporeal membrane oxygenation (ECMO), open constricted or blocked blood vessels, treat septic shock and irreversible shock due to sepsis and/or severe blood loss irreversible septic shock, treat dilutional coagulopathy of severe blood loss and bleeding with loss of clotting factors, and replace lost volume in childhood diseases that cause hypovolemia or severe hypovolemia, treat traumatic brain injury, burns, diagnosis of diseases and methods of making and using said compositions.

A substrate unit for cryopreservation of a biological sample includes: a substrate platform having on a front side thereof a cultivation surface for receiving the biological sample; a first chamber including the cultivation surface of the substrate platform, wherein the first chamber is configured for receiving a cultivation liquid; a second chamber configured for receiving a temperature control medium; and a chamber bracket for receiving the substrate unit in a cryopreservation device in a pivotable manner, wherein the first and second chambers are connected to each other in an adjacent manner, and the substrate platform forms a separating wall between the first chamber and the second chamber, and wherein the back side of the substrate platform faces the second chamber. The invention further relates to a cryopreservation device, wherein the substrate unit is arranged in a rotatable manner, and a method for the cryopreservation of a biological sample.

A method for the isolation, storage and retrieval of mature retinal cells is disclosed. The Method is applicable to adult mammalian cone cells, and more particularly human cone cells, and to healthy as well as pathological or otherwise altered cone cells. A kit for the isolation, storage and retrieval of mature retinal cells is also described.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, and an in vitro fertilization medium.

Disclosed are compositions and methods for the preservation, storage, and transport of living biological tissues, organs, and populations of isolated cells. In particular, the disclosed compositions and processes permit mammalian cells, tissues, and organs to be harvested from suitable donor animals, stored for prolonged periods, and transported to the site of recipient implantation, all without significant loss of cell viability, biological activity, and/or tissue integrity.

A repaired ex vivo organ suitable for transplantation in a human, said repaired ex vivo organ having undergone ex vivo organ perfusion for a maintenance period, wherein said organ had been assessed as being unsuitable for transplantation into a human before the maintenance period and was determined to be suitable for transplantation after the maintenance period.

An object of the present invention is to provide a means capable of stably storing an extracellular vesicle. The present invention relates to a storage stabilizer for an extracellular vesicle, which contains a copolymer containing a monomer unit derived from vinylpyrrolidone and a monomer unit derived from vinyl acetate, or a polymer having a monomer unit derived from vinylpyrrolidone such as polyvinylpyrrolidone and relates to a storage stabilization method for an extracellular vesicle, which includes cryopreserving an extracellular vesicle in the presence of the polymer.

The present invention relates to a composition for preserving cells and a method for preserving cells and, more specifically, to: a composition for preserving cells, containing, as active ingredients, plant-derived recombinant human serum albumin and plant peptides, wherein the composition maintains a high cellular survival rate while maintaining animal-free and xeno-free properties and is stable without changes in cellular morphology or surface expression factors in the short-term preservation of cells such as stem cells or primary cultured cells; and a method for preserving cells by using the same.