The invention refers to a composition for the fixation of histological preparations, comprising a solution of glyoxal, wherefrom acids, normally present in the commercially available glyoxal, have been removed. The composition of the invention provides an optimal fixative for the preservation of structure, antigenic components and nucleic acids in tissues. The acid-free glyoxal has limited toxicity, is not considered as a carcinogenic agent and represents a valid alternative to formalin for the fixation of tissues and cells.

Provided herein are peptide-based hydrogels, or neutral multidomain peptide hydrogel (NMDP), as well as uses thereof. The uses include encapsulating cells to induce quiescence for long-term storage and administering to a subject to induce collagen deposition and macrophage infiltration. The disclosed hydrogel is useful for the preservation of stem cells, including maintaining their quiescence and differentiation potential.

A system and method for cryopreservation of cells or spheroids of cells including obtaining a suspension of the isolated mammalian cells in a medium; applying the suspension to a fibrous substrate comprising a network of interconnected cellulosic fibers defining a plurality of pores therein and allowing the suspension to absorb into the fibrous substrate such that the isolated mammalian cells penetrate and settle to an interior of the fibrous substrate; and cooling the fibrous substrate to or below a temperature of −80° C.

The present disclosure provides a cell system comprising eukaryotic cells in a hydrogel comprising nanofibrillar cellulose in cell storage medium at a temperature in the range of 0-25° C. The present disclosure also provides a method for storing eukaryotic cells, the method comprising providing eukaryotic cells, providing nanofibrillar cellulose, combining the cells and the nanofibrillar cellulose to form the cell system, and storing the cell system at a temperature in the range of 0-25° C.

The present disclosure provides a method for freeze-drying cells in a hydrogel comprising nanofibrillar cellulose, the method comprising providing a hydrogel comprising nanofibrillar cellulose, providing cells, combining the cells and the hydrogel comprising nanofibrillar cellulose to form a cell system, and freeze drying the cell system to obtain dried cells in a hydrogel comprising nanofibrillar cellulose. The present disclosure also provides a freeze-dried hydrogel comprising nanofibrillar cellulose and cells.

A viable disc regenerative composition has a micronized material of nucleus pulposus and a biological composition made from a mixture of mechanically selected allogeneic biologic material derived from bone marrow having non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components; and wherein the mixture is compatible with biologic function and further includes non-expanded whole cells. The biological composition is predisposed to demonstrate or support elaboration of active volume or spatial geometry consistent in morphology with that of disc tissue. The viable disc regenerative composition extends regenerative resonance that compliments or mimics disc tissue complexity.

The present invention relates to a method for cryopreserving and thawing an organ-on-a-chip that has a three-dimensional tissue structure and function. Specifically, the present invention relates to a method for cryopreserving and thawing an organ-on-a-chip that comprises cells and hydrogel and has a microchannel structure, whereby the organ-on-a-chip has an excellent effect of being able to maintain the structure and function of the three-dimensional tissue before and after cryopreservation and thawing.

A method for treating tissue to form a dry tissue component that is readily rehydrated and does not require rinsing prior to implantation in the human body. The method comprises pretreatment and fixation steps that include penetrating agent molecules having a flexible backbone and at least one polar group; the steps further include cations and anions to enhance integration of penetration agent molecules into and bonding to tissue component structural molecules to provide resistance to cracking of the dry tissue component during bending.

A cryopreservation composition generally includes a cryoprotective agent and a laser absorber that includes a metal. Rewarming a cryopreserved specimen that includes the cryopreservation composition includes subjecting the cryopreserved specimen to a laser pulse effective to heat the laser absorber sufficiently to thaw the cryopreserved biospecimen.

The present invention provides a composition for treating a pathological condition in a body part of a patient in needed thereof, comprising an effective amount of a birth tissue elute or birth tissue particulates. A method for preparing the birth tissue elute, for example, an umbilical cord elute, is also provided. A method for treating a pathological condition in a body part of a patient in needed thereof is further provided.