A method of preparing isolated body tissue (10) for cryogenic storage, the method comprising a step of actively infusing the body tissue (10) with a cryoprotectant (20). Additionally, a kit for preparing umbilical cord tissue (10) for cryogenic storage, comprising a cryoprotectant (20) and infusion means (22) for actively infusing the umbilical cord tissue (10) with the cryoprotectant (20).

Systems and methods of the invention generally relate to prolonging viability of bodily tissue, through the use of pressurizer element operable in a sealed organ transport system purged of air and filled with preservation fluid. The pressurizer element is operable to capture and maintain pressure from a fluid-fill line during set-up of the container. Increased pressure within the container reduces edema in the organ during storage and transport by providing a compressive force on the organ.

Systems and methods of the invention generally relate to prolonging viability of bodily tissue, especially lung tissue, through the use of an expandable accumulator to maintain a constant pressure within the lumen of the organ even during external pressure fluctuations due to, for example, flight. Systems and methods may include prolonging donor organ viability in storage through the use of an organ container that mimics the geometry and orientation of the organ in vivo.

The present invention relates to a gas delivery device comprising a gas releasing molecule and a gas permeable membrane. The invention also relates to the use of the gas delivery device for delivery of gas to an extracorporeal transplant, extracorporeal cells, a brain-dead transplant donor or foodstuff and in therapy. The invention further relates to the use of a gas permeable and liquid and solid impermeable membrane to separate a gas releasing molecule and its non-gaseous degradation products from an extracorporeal transplant, extracorporeal cells, a brain-dead transplant donor or foodstuff.

An organ care system including a blood product heater that includes a heater inlet, a channel, and a heater outlet, wherein the heater inlet includes an inside cross-sectional area and an inside cross-sectional shape configured to flow a blood product into the blood product heater, wherein the heater outlet comprises an inside cross-sectional area and an inside cross-sectional shape configured to flow the blood product out of the blood product heater, and wherein the channel extends between the heater inlet and the heater outlet and is formed between a first flow channel plate and a second flow channel plate; and a first heater thermally coupled to the first flow channel plate configured to heat the first flow channel plate; and a pump configured to circulate a heated blood product to an organ being preserved.

Biological tissue is packaged to be regenerated before grafting in a vial sealed under vacuum and comprising a biological tissue matrix. A method for producing said vial includes placing a treated ex-vivo tissue sample in an open rigid vial and placing the vial in a lyophilizer. A lyophilization process is performed under vacuum to convert the treated ex-vivo tissue sample into a biological tissue matrix. The vial is hermetically sealed with closing means, inside the lyophilizer under vacuum. The sealed vial is then removed from the lyophilizer.

One aspect of the invention provides a method for preparing a vein graft. The method includes: applying a tissue passivation agent to a resected anatomical vessel; placing the resected anatomical vessel in a chamber; and allowing the tissue passivation agent to cross-link while the resected anatomical vessel is in the chamber.

Disclosed herein are cellular cassettes for the storage, collection, and analysis of biological samples. The cellular cassette can enable easy sample collection and sealing of microwell arrays with semi-permeable membrane for stable storage and future processing of single cells. Also disclosed herein are systems and kits comprising one or more described cassettes. The described cassettes, systems, and kits can be used to create barcoded, single-cell sequencing libraries. Further described herein are methods of using the cassettes, systems, and kits.

Disclosed are an in-vitro cardiopulmonary combined perfusion system and perfusion method. The in-vitro cardiopulmonary combined perfusion system includes an organ cabin, a circulation cabin, a control cabin, a simple breathing cabin, a display and control panel, and a base. The organ cabin is connected with the circulation cabin, the control cabin and the simple breathing cabin. The control cabin is connected with the display and control panel. The organ cabin, the circulation cabin, the control cabin, the simple breathing cabin, and the display and control panel are mounted on the base.

A cell cryopreservation pretreatment operation plate includes a planar operation portion, a first recess for storing a treatment solution, a second recess for storing a treatment solution, a third recess for storing a treatment solution, and a fourth recess for storing discarded solutions. The planar operation portion includes, on a surface thereof, a first circular segment, second circular segment, and third circular segment for placing treatment solutions, an enlarged first circular segment enclosing the first circular segment, an enlarged second circular segment enclosing the second circular segment, and an enlarged third circular segment enclosing the third circular segment.