A blood storage system comprising: a collection vessel for red blood cells; an oxygen or oxygen and carbon dioxide depletion device; a storage vessel for red blood cells; tubing connecting the collection vessel to the oxygen or oxygen and carbon dioxide depletion device and the oxygen or oxygen and carbon dioxide depletion device to the storage vessel; and a gamma or X-ray irradiating device is used to irradiate red blood cells stored in the vessel, storing red blood cells under anaerobic conditions.

A living body specimen transport device for receiving multiple living body specimens has a frame, a rotating bracket, and a storage assembly. The rotating bracket can be rotated with respect to the frame. The storage assembly can receive a container with a living body specimen and be rotated with respect to the rotating bracket. A center of gravity of the storage assembly is lower than a pivoting point where the rotating bracket is mounted on the frame and a pivoting point where the storage assembly is mounted on the rotating bracket. With such structure, even when the living body specimen transport device is vibrated and shaken during transporting and then the frame of the living body specimen transport device is tilted or turned over, the rotating bracket and the storage assembly can rotate to be vertical by themselves, which keeps the living body specimen being soaked in the preservation solution.

A method of decellularizing a tissue includes disposing the tissue within a device, the device. The device includes a container, first and second electrodes disposed within the container and defining a space between the first and second electrodes to receive the tissue, a perfusion pump, and a conduit connected to the perfusion pump to transport a decellularization composition from the pump into the tissue. The method further includes disposing the decellularization composition within the container to surround the tissue and contact the first and second electrodes, applying an electric potential across the first and second electrodes, and perfusing the decullarization composition into the tissue.

Large volume cellular material may be preserved by combining the cellular material with a cryoprotectant formulation/medium/solution containing at least one mNP and then subjecting the cellular material to a vitrification preservation protocol including nanowarming. This preservation method is particularly effective for cartilage tissues.

A method for freeze-drying a biological sample of mammalian cells or tissue including placing a biological sample on or in a structure to increase a temperature of the biological sample and with the biological sample in a closed chamber applying a vacuum to the chamber to lower a pressure within the chamber, cooling the chamber to lower a temperature within the chamber and applying heat to the biological sample within the chamber. The biological sample can include one or more of stem cells, hematopoietic stem cells, mesenchymal stem cells, embryonic stem cells, induced pluripotent stem cells, sperm, oocytes, embryos, ovarian tissue, uterine tissue or testicular tissue.

A DSC (Differential Scanning calorimetry) electrode system capable of applying an electric field includes a differential scanning calorimeter, a computer, a signal generator, a self-pressurization liquid nitrogen tank and a reference crucible, wherein the self-pressurization liquid nitrogen tank is connected to the differential scanning calorimeter and used for controlling temperature in real time; the differential scanning calorimeter is connected to the computer and used for transmitting signals and recording experiment results. The DSC electrode system also includes a microelectrode crucible that includes a ceramic crucible, a ceramic crucible cover, welding spots, two electrodes and electrode wires, wherein the two electrodes are respectively fixed in the ceramic crucible; a gap is reserved between the electrodes and used for storing a tested sample; the welding spots are reserved at upper ends of the electrodes.

Methods and compositions of storing a biological material are described herein. In some embodiments, these methods provide one or more advantages over current methods. For example, methods described herein can be used to prepare and process biological materials for storage at elevated temperatures. In one aspect, a method of storing a biological material comprises providing a preservation composition and exposing the preservation composition to electromagnetic radiation to form an amorphous solid matrix containing the biological material. In some embodiments, the method further comprises monitoring temperature of the preservation composition during the exposure to the electromagnetic radiation.

Embodiments include exposing blood to a pulsed magnetic field. Embodiments include exposing blood to a pulsed magnetic field as a means of improving blood viscosity management, blood oxygenation, and banked blood shelf life. Various other embodiments are also included herein.

Cryoprotective compositions with magnetic nanowires and cryoprotective agents are used to cryopreserve biomaterials. The biomaterials in the cryoprotective composition are rapidly and uniformly heated during warming to minimize damage due to cracking and/or devitrification. The nanowires are CoFe nanowires. The nanowires may be aligned parallel to the alternating magnetic field to increase the specific absorption rate of the nanowires. The methods include warming the cryopreserved biomaterial in the cryopreserved composition at a rate higher than the critical warming rate using an alternating magnetic field.

Disclosed are apparatuses and methods for irradiating a perfusate. The apparatus includes a tank which defines a first chamber. A separator is located inside the first chamber. The separator defines a second chamber. The first chamber and the second chamber are concentric and have substantially annular cross sections, each having at least one diameter and a substantially common longitudinal axis. A perfusate is introduced into the first chamber by an inlet. A UV radiation-emitting device is disposed inside the second chamber for providing irradiation to the perfusate. Irradiated perfusate is removed from the tank by an outlet. Other apparatuses and systems are described and methods for inactivating micro organisms by performing EVP and irradiating the perfusate.