Provided herein is a method of providing a food product. The method includes diluting a liquid concentrate composition with water to provide the food product. The liquid concentrate composition includes steviol glycosides, a stabiliser including xanthan and/or iota-carrageenan, and water. The steviol glycosides include rebaudioside M in an amount of about 800 ppm or more by total weight of the liquid concentrate composition. The pH of the liquid concentrate composition is less than about 7. Also provided herein is a food product obtainable from the method, a kit for providing the liquid concentrate composition, and a method of providing a liquid concentrate composition.

A method for making highly soluble steviol glycoside compositions. The method can provide steviol glycoside solutions having a high concentration of dissolved steviol glycosides, and which exhibit long-term resistance to precipitation. Various steviol glycoside compositions containing solubilized rebaudioside M and rebaudioside A are described, including both liquid and dry compositions.

A method of screening for a stevia plant with high steviol glycoside content, that includes detecting from the genome of a test stevia plant the presence and/or the absence of the following genetic feature: (1) homozygous for the allele wherein the base at the position corresponding to position 290 of SEQ ID NO: 1 is T, and the presence and/or the absence of the following genetic feature: (2) homozygous or heterozygous for the allele wherein the base at the position corresponding to position 40 of SEQ ID NO: 2 is A.

Enhanced water beverages containing a steviol glycoside composition, a mogroside composition, a miracle fruit composition, and at least one vitamin and/or electrolyte are provided.

Steviol glycoside compositions having improved sweetness and flavor profiles are described.

The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog. V), Mogroside VI (Mog. VI), Iso-Mogroside V (Isomog. V), and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.

Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.

Methods of preparing highly purified steviol glycosides. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various starting compositions to target steviol glycosides. The highly purified steviol glycosides are useful as non-caloric sweetener, flavor enhancer, sweetness enhancer, and foaming suppressor in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

A sensory modifier can provide a steviol glycoside composition with modified sensory attributes, e.g., reduced sweetness linger or reduced bitterness. One such sensory modifier includes a salt fraction and an acid fraction. The salt fraction may include a salt of a monocaffeoylquinic acid and/or a salt of a dicaffeoylquinic acid. The acid fraction may include a monocaffeoylquinic acid and/or a dicaffeoylquinic acid. The salt fraction is preferably at least 50 wt % of sensory modifier.

A flavor composition is provided. The composition includes aromadendrin 3-acetate; and at least one sweetness modifier selected from the group consisting of steviol glycosides, mogrosides, dihydrochalcones, sweet proteins and combinations thereof.