Nutritive polypeptides are provided herein. Also provided are various other embodiments including nucleic acids encoding the polypeptides, recombinant microorganisms that make the polypeptides, vectors for expressing the polypeptides, methods of making the polypeptides using recombinant microorganisms, compositions and formulations that comprise the polypeptides, and methods of using the polypeptides, compositions and formulations.

An object of the present invention is to preserve the total content of chlorophyll in an extract while increasing the extraction yield of a Spirulina extract. Another object of the present invention is to provide a pharmaceutical composition and health functional food for prevention or treatment of degenerative cranial nerve diseases containing a Spirulina extract as an active ingredient, and to provide a method for treating degenerative cranial nerve diseases using a Spirulina extract. The present invention provides a method for producing a Spirulina extract including the steps of (a) preparing a Spirulina powder; (b) adding 50% to 80% of ethanol to the Spirulina powder and performing an ultrasonic pretreatment at 15° C. to 35° C.; (c) extracting a Spirulina extract at 50° C. to 80° C.; (d) vacuum-concentrating the Spirulina extract; and (e) freeze-drying the vacuum-concentrated Spirulina extract.

Provided is a Pichia pastoris mutant strain for expressing an exogenous gene. Specifically, provided is a Pichia pastoris mutant strain comprising, with respect to Pichia pastoris mutant strain GS115 or CICC32806, one or more of the following six mutations: BQ9382_C1-2260, EKK deletions at positions 308-310, a hypothetical protein; BQ9382_C1-3800, E129K, 60S ribosomal subunit assembly/exported protein LOC1; BQ9382_C1-5700, I312M, mitochondrial external NADH dehydrogenase, type II NAD(P)H:quinone oxidoreductase; BQ9382_C2-3950, Q145X, an essential protein having a binding partner Psr1p and used for completely activating a general stress response; BQ9382_C3-2220, E188K, a hypothetical protein; and BQ9382_C3-4370, W196X, orotidine 5\′-phosphate decarboxylase. The provided Pichia pastoris mutant strain is an effective commonly employed host for exogenous expression, and can efficiently express different proteins, especially phospholipase and lipase.

The present invention relates to a process for the cultivation of unicellular red algae (URA) optimized for the valorization of the culture products, both the biomass obtained, the phycocyanins extracted therefrom or other culture products such as porphyrins or protein extracts.

The present invention relates to a process for the cultivation of unicellular red algae (URA) optimized for the valorization of the culture products, both the biomass obtained, the phycocyanins extracted therefrom or other culture products such as porphyrins or protein extracts.

The present invention discloses novel exquisite VCF as well as the inventive methodology for its production. It involves the disruption of the structure of the natural vegan compatible raw material and creation of the VCF structure by sophisticated inventive processing taking advantage of relevant properties of vegan compatible raw material. VCF contain all nutrients and active ingredients of the vegan compatible raw materials used. The composition of such inventive nutrition is adaptable to common and personalized nutritional requirements.

The present invention discloses novel exquisite VCF as well as the inventive methodology for its production. It involves the disruption of the structure of the natural vegan compatible raw material and creation of the VCF structure by sophisticated inventive processing taking advantage of relevant properties of vegan compatible raw material. VCF contain all nutrients and active ingredients of the vegan compatible raw materials used. The composition of such inventive nutrition is adaptable to common and personalized nutritional requirements.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.

Methods of production of edible filamentous fungal biomat formulations are provided as standalone protein sources and/or protein ingredients in foodstuffs as well as a one-time use or repeated use self-contained biofilm-biomat reactor comprising a container with at least one compartment and placed within the compartment(s), a feedstock, a fungal inoculum, a gas-permeable membrane, and optionally a liquid nutrient medium.