The present disclosure provides tobacco Nic1b locus and associated genes. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

The present disclosure provides tobacco Nic1b locus and associated genes. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

The invention relates to an aerosol generating material comprising a blend, the blend comprising at least 20% lamina tobacco, and the material is in the form of discrete particles. The invention al relates to articles for an aerosol provision system, the articles comprising a first aerosol generating material and a second aerosol generating material.

The invention relates to an aerosol generating material comprising a blend, the blend comprising at least 20% lamina tobacco, and the material is in the form of discrete particles. The invention al relates to articles for an aerosol provision system, the articles comprising a first aerosol generating material and a second aerosol generating material.

A tobacco preparation method, where a tobacco material is prepared through at least one extrusion process including compressing the material with an increase in pressure and temperature and mechanically processing and abruptly flash drying the material at an extruder outlet, wherein the tobacco material includes a tobacco lamina material, where a tobacco preparation device includes a dosing conveyor and an extruder which compresses a tobacco material with an increase in pressure and temperature and mechanically process and abruptly flash dries the material at the extruder outlet, wherein the components are designated as a unit which can be modularly delimited for processing a tobacco material including a tobacco lamina material, where an arrangement of several tobacco preparation devices and a smoking article or cigarette manufacturing device includes such a device or such a device arrangement which is connected upstream of a cigarette maker or a secondary unit as a tobacco preparation module.

A tobacco preparation method, where a tobacco material is prepared through at least one extrusion process including compressing the material with an increase in pressure and temperature and mechanically processing and abruptly flash drying the material at an extruder outlet, wherein the tobacco material includes a tobacco lamina material, where a tobacco preparation device includes a dosing conveyor and an extruder which compresses a tobacco material with an increase in pressure and temperature and mechanically process and abruptly flash dries the material at the extruder outlet, wherein the components are designated as a unit which can be modularly delimited for processing a tobacco material including a tobacco lamina material, where an arrangement of several tobacco preparation devices and a smoking article or cigarette manufacturing device includes such a device or such a device arrangement which is connected upstream of a cigarette maker or a secondary unit as a tobacco preparation module.

A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region/leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.

The present disclosure relates to an ultra-low nicotine smoking article. The smoking article includes a medium part and a filter part, in which the medium part includes a tobacco medium including flue-cured tobacco, dry ice expanded tobacco, and expanded stems, the tobacco medium includes 50% by weight (wt %) to 60 wt % of flue-cured tobacco, 20 wt % to 30 wt % of dry ice expanded tobacco, and 10 wt % to 30 wt % of expanded stems based on the total weight, and the tobacco medium has a nicotine content of 0.01% to 0.05%.

The present disclosure provides tobacco Nic1b locus and associated genes (e.g., a group of ERF genes). Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

The present disclosure provides tobacco Nic1b locus and associated genes (e.g., a group of ERF genes). Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.