The invention relates to tobacco wax compositions suitable for use in a vaporizer. The tobacco wax may comprise additional excipients including vapor agents, penetration agents, buffer agents, and rheological agents. The composition contains nicotine. The tobacco wax composition leaves a minimum of residue in the vaporizer when used. In another aspect, the invention relates to a portion-sized container (pod) of a tobacco wax composition for administration to a mammal or person. The pod is intended for use in a personal (or other) vaporizer.

A method of modifying the moisture level of (e.g., dehydrating) a tobacco plant or portion thereof, a tobacco-derived material, a tobacco product, or a tobacco additive is provided herein. The methods of dehydration disclosed herein can provide various benefits relative to traditional drying techniques, including providing retention of various beneficial components present in green tobacco (e.g., organoleptic compounds and proteins). Smoking articles and other tobacco products including such dehydrated tobacco materials are also provided.

Rihulose-1,5-bisphosphate oxygenase (RuBisCO) protein fibers and a method of producing them are disclosed herein. The method of producing one or more RuBisCO protein fibers including obtaining RuBisCO, for example from tobacco, combining the RuBisCO with one or more plasticizers, heating the combination of the RuBisCO and the one or more plasticizers up to about 140 degrees C., filtering the heated combination through an about 20 ?m filter, and passing the filtered combination through an orifice to produce one or more RuBisCO protein fibers.

The present disclosure relates to a method for manufacturing a sheet for a smoking article, and more particularly, to a method for manufacturing a sheet for a smoking article, the method comprising the steps of: putting a tobacco material into an aqueous solvent, and soaking and pressurizing it to separate a solid part and a liquid part (S1); removing the liquid part, forming a sheet with the solid part, and then drying and pulverizing the sheet (S2); preparing a slurry by mixing pulp with the pulverized sheet (S3); and injecting the slurry to form a sheet (S4), wherein the sheet has a reduced specific component, and the specific component includes one or more selected from the group consisting of nicotine and nitrate. A smoking article comprising the sheet manufactured by the above manufacturing method is characterized by having a reduced specific component and excellent flavor.

The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of nicotine and may have other benefits, e.g., lightening the color of the protein-enriched extracts. The methods generally include treatment with peracetic acid or hydrogen peroxide and filtrations. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant of the Nicotiana species, wherein the protein composition is characterized by relatively low nicotine content.

The invention relates to a method for the preparation of a homogenized tobacco material, said method comprising: pulping and refining cellulose fibres to obtain fibres having a mean size per weight comprised between about 0.2 millimetres and about 4 millimetres; grinding a blend of tobacco of one or more tobacco types to tobacco particles having a mean size per weight comprised between about 0.03 millimetres and about 0.12 millimetres; Combining the cellulose fibres with the tobacco particles and with a binder to form a slurry; homogenizing the slurry; adding asparaginase to the slurry; andforming the homogenized tobacco material from the slurry, wherein the homogenized tobacco material comprises from about 1 percent and about 5 percent in dry weight basis of the binder.

Provided is a technique for increasing an amount of solanone in a tobacco leaf. A method for increasing an amount of solanone in a tobacco leaf in accordance with an embodiment of the present invention includes a heating step of heating a tobacco leaf for not less than 30 minutes at a temperature of not lower than 80 C. in the presence of water.

his invention provides a method for efficiently isolating and purifying neophytadiene from leaf tobacco with high yield and purity. The method involves: adding an aprotic solvent to leaf tobacco for extraction and removing residue to obtain an extraction liquid; adding an acidic aqueous solution for liquid-liquid extraction and removing the aqueous layer; adding a protic solvent for further liquid-liquid extraction and removing the solvent layer; evaporating the solvent to obtain dried matter; adding a protic solvent to form a solution; removing precipitates to obtain a supernatant; replacing the solvent with an aprotic solvent to form a new solution; and isolating and purifying neophytadiene using normal phase HPLC.

The present disclosure describes methods of obtaining and/or deriving a sugar-enriched extract from plants of the Nicotiana species and methods for incorporation of such extracts into various tobacco compositions. One exemplary method for preparing a sugar-enriched extract comprises contacting Nicotiana plant material with a solvent and extracting sugars into the solvent to form a liquid sugar-containing extract; separating a solid extracted plant material from the liquid sugar-containing extract; clarifying the liquid sugar-containing extract to form a clarified sugar-containing extract and a solids fraction; and isolating the liquid sugar-containing extract to provide a sugar-enriched extract comprising a combined weight of fructose and glucose of at least about 10% by dry weight. In certain embodiments, the sugars of the extract comprise at least about 50% by weight fructose.

A method of modifying the moisture level of (e.g., dehydrating) a tobacco plant or portion thereof, a tobacco-derived material, a tobacco product, or a tobacco additive is provided herein. The methods of dehydration disclosed herein can provide various benefits relative to traditional drying techniques, including providing retention of various beneficial components present in green tobacco (e.g., organoleptic compounds and proteins). Smoking articles and other tobacco products including such dehydrated tobacco materials are also provided.