An apparatus for carrying out polarization resolved Raman spectroscopy on a sample (11), in particular a crystalline or polycrystalline sample, the apparatus comprises: at least one light source (13, 87, 93, 95, 97), in particular at least one laser, for providing excitation radiation to a surface of the sample (11), an optical system which is configured to simultaneously collect at least one on-axis Raman beam (21, 109) and at least one off-axis Raman beam (23, 111) from Raman light scattered by the sample (11) in response to exposing the surface to the excitation radiation, the at least one on-axis Raman beam (21, 109) being scattered from the sample (11) in a direction that is aligned with an optical axis of an objective (41) of the optical system for collecting the at least one on-axis Raman beam (21, 109), the at least one off-axis Raman beam being scattered from the sample in a direction that is inclined with regard to an optical axis of an objective (41) of the optical system for collecting the at least one off-axis Raman beam (23, 111), the optical system comprises at least one polarizer device (25, 113) for generating at least one polarized on-axis Raman beam (31, 33) from the at least one on-axis Raman beam (21, 109) and at least one polarized off-axis Raman beam (35) from the at least one off-axis Raman beam (23, 111), and the optical system comprises at least one spectrometer (37, 47 81, 83, 85) for generating, in particular simultaneously, an optical spectrum from each of the at least one polarized on-axis Raman beam (31, 33) and the at least one polarized off-axis Raman beam (35).

A stimulated Raman scattering (SRS) spectrometer for real-time, high-resolution molecular analysis of gases is based on two hollow-core fibres illuminated by a single high-power, short-pulse laser pump. The first fibre is prefilled with high-concentration target gases. Interaction of each target gas inside the first fibre, with the laser pump, generates Raman signals corresponding to the target gases. The combined beam of the Raman signals and the pump laser beam is directed into the second fibre containing the measured target gases. Interaction of each target gas with the combined beam generates the Stimulated Raman Growth (SRG), i.e., amplification of the Raman signal, which is proportional to the corresponding target gas concentration. A receiver subsystem receives the beam from the second fibre, spectrally separates it to wavelengths corresponding to each target gas, extracts the SRG value corresponding to each target gas and calculates the concentration of each target gas.

A system and method for characterization and/or calibration of performance of a multispectral imaging (MSI) system equipping the MSI system for use with a multitude of different fluorescent specimens while being independent on optical characteristics of a specified specimen and providing an integrated system level test for the MSI system. A system and method are adapted to additionally evaluate and express operational parameters performance of the MSI system in terms of standardized units and/or to determine the acceptable detection range of the MSI system.

An optical sensor device may comprise an optical sensor comprising a set of sensor elements; an optical filter comprising one or more channels, wherein each channel, of the one or more channels, is configured to pass light associated with particular wavelengths to a subset of sensor elements, of the set of sensor elements, of the optical sensor; a phase mask configured to distribute a plurality of light beams associated with a subject in an encoded pattern on an input surface of the optical filter; and one or more processors. The one or more processors may be configured to obtain, from the optical sensor, sensor data associated with the subject and determine, based on the sensor data, spectral information associated with the subject. The one or more processors may determine, based on the sensor data and information associated with the encoded pattern, spatial information associated with the subject.

An optical system includes an optical element and an optical filter with a first set of channels and a second set of channels respectively associated with a first region and a second region of the optical filter. The optical element causes first light beams and second light beams associated with a subject to respectively fall incident on the first region within a first incidence angle range and on the second region within a second incidence angle range. A first channel, of the first set of channels, passes, based on the first incidence angle range, a set of the first light beams that are associated with a first subrange of a particular wavelength range. A second channel, of the second set of channels, passes, based on the second incidence angle range, a set of the second light beams that are associated with a second subrange of the particular wavelength range.

A spectroscopic module includes a plurality of beam splitters; a plurality of bandpass filters disposed on one side in a Z direction with respect to the plurality of beam splitters; a light detector disposed on the one side in the Z direction with respect to the plurality of bandpass filters and includes a plurality of light receiving regions; a first support body supporting the plurality of beam splitters; a second support body supporting the plurality of bandpass filters; and a casing including a third wall portion integrally formed with the second support body. The first support body is attached to the third wall portion such that an outer surface of the first support body is in contact with an inner surface of the third wall portion in a state where the position is defined by a plurality of positioning pins and a plurality of positioning holes.

A Raman spectrometer arrangement comprising a Raman spectrometer 1 having a laser 1001 for illuminating a sample S and a spectrometer accessory 4 configured to be mounted on the spectrometer, wherein the spectrometer accessory comprises a surface configured to receive the sample S. The Raman spectrometer arrangement is configured to operate in at least a first configuration and a second configuration, wherein the first configuration is such that the laser 1001 illuminates the sample S before reaching a level of the surface and the second configuration is such that the laser 1001 reaches the level of the surface before illuminating the sample S.

An imaging system includes: a first light source that emits first light having a spectrum including discrete first frequency components arranged at first frequency intervals; a second light source that emits second light having a spectrum including discrete second frequency components arranged at second frequency intervals, the second frequency intervals being different from the first frequency intervals; a mixing optical system that mixes the first light and the second light to generate third light including at least one optical beat the intensity of which changes at a beat frequency expressed by the difference between at least one of the discrete first frequency components and at least one of the discrete second frequency components; an imaCCging element having a variable sensitivity in an exposure period; and a control circuit that changes the sensitivity of the imaging element at the beat frequency of the at least one optical beat.

The invention provides systems for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation and methods for using the same. The system includes a laser source, collection fibers, a demultiplexer and an optical delay device. All references cited herein are incorporated by reference in their entirety as though fully set forth. Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of-ordinary skill in the art in which this invention belongs.

A contact-type endoscope surface enhanced Raman scattering (SERS) probe includes a gradient-index (GRIN) lens, a transparent substrate adhered to the GRIN lens, and a rough metallic layer adhered to an opposite side of the transparent substrate from the GRIN lens. The GRIN lens focuses light from a Raman spectrometer onto the rough metallic layer, and the rough metallic layer is positioned at the distal end of the contact-type endoscope SERS probe.