The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

A mesoscale fluidic system comprises a substrate having a sample chamber and an analysis chamber. The sample chamber comprises a cell permeable filter defining a sample application compartment and a conditioning medium compartment. The sample chamber has a sample inlet port in the sample application compartment. The analysis chamber has an entry port and an exit port. The conditioning medium compartment is in fluid communication with the entry port of the analysis chamber via a channel. The sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. The mesoscale fluidic system is suited for analysing cellular motility in a sample. Also disclosed is a method of estimating the quantity of motile cells in a sample and a method of extracting motile cells from non-motile cells.

An analytical device for analysing ions is provided comprising a separator 2 for separating ions according to a physico-chemical property and an interface 3 comprising one or more ion guides. A quadrupole rod set mass filter 4 is arranged downstream of the interface 3. A control system is arranged and adapted: (i) to transmit a first group of ions which emerges from the separator 2 through the interface 3 with a first transit time t1; and (ii) to transmit a second group of ions which subsequently emerges from the separator 2 through the interface 3 with a second different transit time t2.

Various sensors, including particulate matter sensors, are described. One particulate matter sensor includes a self-mixing interferometry sensor and a set of one or more optical elements. The set of one or more optical elements is positioned to receive an optical emission of the self-mixing interferometry sensor, split the optical emission into multiple beams, and direct each beam of the multiple beams in a different direction. The self-mixing interferometry sensor is configured to generate particle speed information for particles passing through respective measurement regions of the multiple beams.

The invention describes a laser sensor module. The laser sensor module comprises at least a first laser (111) being adapted to emit a first measurement beam (111) and at least a second laser (112) being adapted to emit a second measurement beam (112). The laser sensor module further comprises an optical device (150) being arranged to redirect the first measurement beam (111) and the second measurement beam (112) such that the 5 first measurement beam (111) and the second measurement beam enclose an angle between 45 and 135. The laser sensor module comprises one detector (120) being adapted to determine at least a first self-mixing interference signal of a first optical wave within a first laser cavity of the first laser (111) and at least a second self-mixing interference signal of a second optical wave within a second laser cavity of the second laser (112). This configuration 10 enables determination of an average velocity of the particles despite of the fact that it is not possible to determine the components of the velocity vector. The introduced error by means of statistical variations is acceptable because the number of detected particles scales with the cubic root of the particle velocity. The invention further describes a particle sensor (100) comprising such a laser sensor module, a corresponding method and computer program 15 product. The invention enables a simple and low-cost particle sensor (100) for detecting small particles based on laser self-mixing interference.

A search device updates positions and momentums of a plurality of virtual particles, for each unit time from an initial time to an end time. The search device, for each unit time, calculates, for each of the particles, a position at a target time of a corresponding particle, calculates, for each of a plurality of nodes, a first accumulative value by cumulatively adding positions at the target time of two or more particles corresponding to outgoing two or more directed edges, calculates, for each of the nodes, a second accumulative value by cumulatively adding positions at the target time of two or more particles corresponding to incoming two or more directed edges, and calculates, for each of the particles, a momentum at the target time of a corresponding particle based on the first accumulative value and the second accumulative value.

A method for determining interaction between cellular bodies and a functionalized wall surface comprises: obtaining a sequence of images representing manipulating cellular bodies in a holding space having a functionalized wall surface configured to bind the cellular bodies that includes applying a force; tracking locations of pixel groups in respective images out of the sequence of images, each pixel group in the images representing a cellular body out of the cellular bodies, the locations in the respective images defining a trajectory of the cellular body moving relative to the functionalized wall surface; determining one or more speed values of the cellular body at one or more locations of the trajectory, the one or more speed values being higher than zero; and, classifying that the cellular body is attached to the functionalized wall surface based on the one or more speed values and at least one threshold speed value.

The present invention relates to a method for cancer cell separation, and more specifically, relates to a method for cancer cell separation using micro-vibration.

An analytical device for analysing ions is provided comprising a separator 2 for separating ions according to a physico-chemical property and an interface 3 comprising one or more ion guides. A quadrupole rod set mass filter 4 is arranged downstream of the interface 3. A control system is arranged and adapted: (i) to transmit a first group of ions which emerges from the separator 2 through the interface 3 with a first transit time t1; and (ii) to transmit a second group of ions which subsequently emerges from the separator 2 through the interface 3 with a second different transit time t2.