A microfluidic device comprising a channel within a substrate and a condenser or a hydrodynamic focusing chamber along the channel, configured to focus a fluid containing particles of a plurality of sizes. A negative angle deterministic lateral displacement (DLD) array is configured to receive the focused fluid and separate the particles in the focused fluid into three sizes ranges. The negative angle DLD array comprises a plurality of rows of pillars, wherein the rows of pillars are positioned to repeat a pattern every N rows with a shift of M columns, N and M are relatively coprime, and N is greater than 1.

A microfluidic device comprising a channel within a substrate and a condenser or a hydrodynamic focusing chamber along the channel, configured to focus a fluid containing particles of a plurality of sizes. A negative angle deterministic lateral displacement (DLD) array is configured to receive the focused fluid and separate the particles in the focused fluid into three sizes ranges. The negative angle DLD array comprises a plurality of rows of pillars, wherein the rows of pillars are positioned to repeat a pattern every N rows with a shift of M columns, N and M are relatively coprime, and N is greater than 1.

An apparatus for monitoring a stream of droplets of target material for generating a radiation beam in a radiation source, wherein the apparatus comprises: a target material emitter for creating the stream of droplets of target material, wherein the target material emitter comprises a chamber configured for the target material to pass through before forming the stream of droplets; a first transducer configured to generate acoustic pressures in the chamber, and a second transducer configured to sense the acoustic pressures in the chamber.

An apparatus for monitoring a stream of droplets of target material for generating a radiation beam in a radiation source, wherein the apparatus comprises: a target material emitter for creating the stream of droplets of target material, wherein the target material emitter comprises a chamber configured for the target material to pass through before forming the stream of droplets; a first transducer configured to generate acoustic pressures in the chamber, and a second transducer configured to sense the acoustic pressures in the chamber.

Beads with functionalized material applied to them are exposed to an acoustic field to trap, retain or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes. The acoustic field may be generated, for example, in an acoustic angled wave device or in an acoustic fluidized bed.

A method of foreign object debris discrimination includes illuminating a particle located within a sensing volume with a modulated electromagnetic radiation pulse emitted from a source; receiving one or more electromagnetic radiation return signals that have been scattered by the particle illuminated by the modulated electromagnetic radiation pulse at a detector; mixing, using a controller, the electromagnetic radiation return signal of amplitude I.sub.RS and frequency f.sub.RS with a reference signal of amplitude I.sub.LS and frequency f.sub.RS; analyzing, using the controller, an amplitude of the mixed signal √{square root over (I.sub.LSI.sub.RS)}, and frequency of the mixed signal, f.sub.RS−f.sub.LS; and classifying, using the controller, a particle position, a velocity, and electromagnetic characteristic of the particle based on the amplitude, √{square root over (I.sub.LSI.sub.RS)}, and frequency, f.sub.RS−f.sub.LS of the mixed signal.

A method of foreign object debris discrimination includes illuminating a particle located within a sensing volume with a modulated electromagnetic radiation pulse emitted from a source; receiving one or more electromagnetic radiation return signals that have been scattered by the particle illuminated by the modulated electromagnetic radiation pulse at a detector; mixing, using a controller, the electromagnetic radiation return signal of amplitude I.sub.RS and frequency f.sub.RS with a reference signal of amplitude I.sub.LS and frequency f.sub.RS; analyzing, using the controller, an amplitude of the mixed signal √{square root over (I.sub.LSI.sub.RS)}, and frequency of the mixed signal, f.sub.RS−f.sub.LS; and classifying, using the controller, a particle position, a velocity, and electromagnetic characteristic of the particle based on the amplitude, √{square root over (I.sub.LSI.sub.RS)}, and frequency, f.sub.RS−f.sub.LS of the mixed signal.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.

The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.