The invention relates to a method for dispensing a liquid sample by means of a dispensing apparatus in which it is determined whether a particle condition is satisfied, wherein the determination comprises checking whether at least one target particle present in a liquid of the liquid sample is contained in a monitoring region of the dispensing apparatus, wherein the monitoring region comprises a discharge region and a buffer region, wherein the buffer region is a region from which the at least one target particle is movable into the discharge region during a time delay between the determination of whether the particle condition is satisfied and an output operation of the dispensing apparatus. The method is characterised in that it is determined that the particle condition is satisfied when the at least one target particle is arranged in the buffer region and no target particle is arranged in the discharge region, and that the liquid sample is dispensed onto a target particle carrier if the particle condition is satisfied.

The invention relates to a method for dispensing a liquid sample by means of a dispensing apparatus in which it is determined whether a particle condition is satisfied, wherein the determination comprises checking whether at least one target particle present in a liquid of the liquid sample is contained in a monitoring region of the dispensing apparatus, wherein the monitoring region comprises a discharge region and a buffer region, wherein the buffer region is a region from which the at least one target particle is movable into the discharge region during a time delay between the determination of whether the particle condition is satisfied and an output operation of the dispensing apparatus. The method is characterised in that it is determined that the particle condition is satisfied when the at least one target particle is arranged in the buffer region and no target particle is arranged in the discharge region, and that the liquid sample is dispensed onto a target particle carrier if the particle condition is satisfied.

A method of automated measurement of motility and morphology parameters of the same single motile sperm. Automated motility and morphology measurements of the same single sperm are performed under different microscope magnifications. The same single motile sperm is automatically positioned and kept inside microscope field of view and in focus after magnification switch. A method of automated non-invasive measurement of sperm morphology parameters under high magnification of imaging. Sperm morphology parameters including subcellular structures are automatically measured without invasive sample staining. A method of automatically selecting sperms with normal motility and morphology and DNA integrity for infertility treatment.

Microfluidic devices and systems are provided. Methods for conducting immune assays with the devices and systems are also provided.

A calibration apparatus comprises estimation circuitry configured to estimate, based on a calibration factor, an estimated number of cells of a first type in a dyed biological sample containing an unknown number of cells. Determination circuitry determines the actual number of cells of the first type in the dyed biological sample. Processing circuitry adjusts the calibration factor. The estimation circuitry is configured with the processing circuitry to estimate the estimated number of the cells of the first type in the dyed biological sample one or more times, based on a different value of the calibration factor for each of the one or more times, until the estimated number of the cells of the first type approaches the actual number of cells of the first type.

To provide a technology of efficiently and effectively sorting microparticles to be sorted from a sample solution. The present technology provides a control device being a device that controls a processing condition when sorting microparticles from a sample liquid flowing through a flow path, the control device provided with a control unit that controls a sorting processing condition on the basis of a content of microparticles to be sorted in the sample liquid. In the control device according to the present technology, the control unit may control the sorting processing condition on the basis of a surviving rate and/or an activation rate of biological particles to be sorted with respect to the sorting processing condition.

The invention provides devices and methods for linked multimodal measurements of individual particles using a mass sensor and an additional sensor.

The invention provides devices and methods for linked multimodal measurements of individual particles using a mass sensor and an additional sensor.

An extracellular potential measurement device includes multiple insulating films each of which is made from an electric insulating material, the insulating films being stacked and bonded to each other; and multiple electrode wires each of which is made from an electroconductive material, the electrode wires being arranged in multiple heights. Each of the electrode wires is interposed between an upper insulating film and a lower insulating film. Each of the insulating films, except for a lowermost insulating film, has an opening penetrating the insulating film. The opening in a lower insulating film has a size that is less than that of the opening in an upper insulating film, the openings in the insulating films being overlapped to form a recess having a size reducing downward, the recess being adapted to store a collection of cells. Each of the electrode wires has an end that is located near an opening in an insulating film that is immediately below the electrode wire, the ends being exposed in the recess.

Disclosed herein are an apparatus for electrically assessing and/or manipulating cells. One aspect is directed to electrically mapping cells on the surface of the semiconductor substrate via cross-electrode impedance measurements. Further according to some aspects, the electrode array allows for spatially addressable electrical stimulation and/or recording of electrical signals in real-time using the CMOS circuitry. Some of these aspects are directed to using an electrode array to perform cell patterning through electrochemical gas generation, and extracellular electrochemical mapping.