Data relating to a particle classified by a centrifugal field flow fractionation device in a preset analysis condition is processed by a data processing apparatus. Inputs of an arbitrary particle diameter and an arbitrary analysis condition are received (Step S101). An elution time of a particle having the particle diameter is calculated based on the input particle diameter and analysis condition (Step S102). The calculated elution time is displayed on a display unit (Step S103).

A method for continuously separating components from a sample includes providing a field-flow fractionation device including: a channel coupled to a flow generator for translocating the sample components along the channel in a first direction, an actuator for translocating the sample components in a second direction, at an angle with the first direction, and an array of electrodes electrically or capacitively connected to an AC power source, operating the actuator so as to translocate the sample components in a second direction at an angle with the first direction, operating the AC power source so as to generate an AC electric field between adjacent rows, and operating the flow generator, collecting sample components from the sample outlets.

A method for continuously separating components from a sample includes providing a field-flow fractionation device including: a channel coupled to a flow generator for translocating the sample components along the channel in a first direction, an actuator for translocating the sample components in a second direction, at an angle with the first direction, and an array of electrodes electrically or capacitively connected to an AC power source, operating the actuator so as to translocate the sample components in a second direction at an angle with the first direction, operating the AC power source so as to generate an AC electric field between adjacent rows, and operating the flow generator, collecting sample components from the sample outlets.

A field-flow fractionation device includes a separation channel, a carrier fluid supplier, a separation membrane, a waste liquid chamber, a cross-flow flow rate adjuster, and a carrier fluid adder. The carrier fluid adder is configured to add, to a flow of a carrier fluid having passed through the separation membrane, a flow of another carrier fluid at a carrier fluid adding position set on an upstream side of the cross-flow flow rate adjuster so that the flow rate of the carrier fluid flowing into the cross-flow flow rate adjuster is larger than the flow rate of the carrier fluid having passed through the separation membrane.

The present invention relates to an asymmetric flow field-flow fractionation device (1) configured to separate a sample (8) of particles (12) dispersed in a liquid mobile phase (11), the device including a fractionation microchannel (2) comprising a sample inlet, a sample outlet, an auxiliary microchannel (3) comprising an auxiliary outlet, a semipermeable membrane (10) separating the fractionation microchannel (2) and the auxiliary microchannel (3), said membrane being permeable to liquid and being configured to maintain the particles (12) in said fractionation microchannel (2), the fractionation microchannel (2) being superimposed on the auxiliary microchannel (3), wherein the device (1) comprises two layers (19), each layer being with a microfabricated recess (14) which thickness (t) is less than 100.sub.IJm, the membrane (10) being mechanically held in between the two layers (19), the recesses (14) respectively defining the fractionation microchannel (2) and the auxiliary microchannel (3) on each side of the membrane (10).

An apparatus is provided. The apparatus may comprise a layer of a microfluidic chip. The layer may comprise a nanoscale deterministic lateral displacement (nanoDLD) array. The nanoDLD array may comprise a plurality of pillars arranged in a plurality of columns. Further, the nanoDLD array may separate particles from a purified fluidic sample associated with a bodily materials of an organism.

A method for purifying at least one target particle from a sample by utilizing a sized-based separation is provided. The method may include detecting the at least one target particle associated with the sample, by utilizing at least one detector molecule in a nanoDLD array. The method may then include separating the detected at least one target particle and the at least one detector molecule from a bump fraction in the sample based on a size of the detected at least one target particle.

The present disclosure describes an apparatus, method, and system of measuring attributes of a viral gene delivery vehicle sample via separation. In an embodiment, the method, system, and computer program product include executing a set of logical operations analyzing a viral gene delivery vehicle sample on a set of analytical instruments, where the set includes at least one separation instrument, at least one static light scattering instrument, and at least two concentration detectors, resulting in a capsid protein mass of the sample, m.sub.A, a modifier mass of the sample, m.sub.B, and a modifier molar mass of the sample, M.sub.B, receiving a capsid protein molar mass of the sample, M.sub.A, from a capsid protein molar mass data source, receiving an injection volume of the sample, v, from an injection volume data source, and executing a set of logical operations calculating a total VGDV particle concentration of the sample, C.sub.A.

The present disclosure describes a method, a system, and a computer program product of analyzing data collected by analytical instruments. In an embodiment, the method, the system, and the computer program product include receiving set-up information, running at least one incomplete analytical method on at least one known sample on at least one analytical instrument with respect to the set-up information, resulting in known sample data, processing the at least one incomplete analytical method with respect to the known sample data, resulting in at least one validated analytical method, and running the at least one validated analytical method on at least one unknown sample on the at least one analytical instrument with respect to the set-up information, resulting in analyzed sample data.

Provided is the processing of sample fluids containing one or more analytes of interest and to methods and devices for separating and/or purifying components of a sample fluid using electric and hydrodynamic forces. Though the fluid processing systems and methods are generally described herein as applied to microfluidics, it will be appreciated that the fluid processing systems may process any fluid volume suitable for use in embodiments described herein. Y-shaped and multiple-branched shaped 2-D EFD devices have been used to separate and/or purify one or more analytes from a mixture. Systems and methods in accordance with various aspects of the present teachings utilize hydrodynamic pressure (e.g., using a pump) to drive the sample liquid from the sample inlet to the separation stream, and can, in some aspects, provide improved control of the movement of the analytes, improved processing times, and decreased buffer depletion.

An asymmetric flow field flow fractionation apparatus 1 is provided with a separation cell having a semi-permeable membrane. Each flow path is connected to the separation cell. A mobile phase that has passed through the semi-permeable membrane of the separation cell flows through a crossflow discharge flow path. The crossflow discharge flow path is provided with a flow controller 14 for adjusting the flow rate. A voltage control unit 332 applies a voltage according to a set flow rate to the flow controller 14 when the set flow rate of the mobile phase of the crossflow discharge flow path is constant, and applies a voltage in which an offset is added to the voltage according to the set flow rate to the flow controller 14 to the flow controller 14 when the set flow rate of the crossflow discharge flow path mobile phase increases. Therefore, even if the set flow rate is changed so as to be increased, the flow rate of the mobile phase moving in the crossflow discharge flow path can be changed so as to correspond to the set flow rate.