A device and method for isolating extracellular vesicles from biofluids is disclosed. A nanoporous silicon nitride membrane is provided with a tangential flow of biofluid. A pressure gradient through the nanoporous silicon nitride membrane facilitates capture of extracellular vesicles from the tangential flow vector of biofluid. Reversal of the pressure gradient results in the release of the extracellular vesicles for subsequent collection.

Techniques for phosphoprotein detection, quantification, and purification using a chip-based pillar array are provided. In one aspect, a method for purifying a protein sample includes: introducing a mixture including the protein sample and an antibody to a nanoDLD array having a plurality of pillars separated by a gap g, wherein the antibody and proteins in the protein sample form antibody-protein complexes having a size that is greater than a size threshold of the nanoDLD array created by the gap g which permits size-based separation of the antibody-protein complexes as the mixture flows through the nanoDLD array; and collecting a purified protein sample containing the antibody-protein complexes from the nanoDLD array. A lab-on-a-chip (LOC) device including the nanoDLD array is also provided.

The invention provides a centrifugal separation type FFF device where a rotor can be rotated at a high speed safely so that particles of a smaller size in a sample liquid can be classified. A field flow fractionation device 11 is provided with: a channel 26 that is attached to the inner circumferential surface 53a of the peripheral portion 53 of a rotor 51 and where a classification flow path 38 is created; flow paths 31, 33, 41, 42, 34, 32 for feeding a sample liquid into and out from the classification flow path 38; and a rotational drive mechanism 28 for rotating the rotational axis 24, wherein a channel installation portion 55 is formed on one side of the peripheral portion 53, and a mass balancer portion 56 for adjusting the mass distribution of the rotor 51 is formed on the other side with the rotor base in between.

A field flow fractionation apparatus includes a separation channel provided with an inlet port and an outlet port at both ends and forming a space through which a carrier fluid flows between the inlet port and the outlet port, a separation membrane which is a wall surface that defines the separation channel and is parallel to a channel flow in which a carrier fluid flows in the separation channel from the inlet port toward the outlet port, and has a property of permeating the carrier fluid and not permeating particles to be separated, and a discharge port that discharges the carrier fluid having permeated through the separation membrane to outside. At least a part of the surface of the separation membrane is an ion exchangeable region in which a functional group having ion exchangeability is modified.

Techniques for phosphoprotein detection, quantification, and purification using a chip-based pillar array are provided. In one aspect, a method for purifying a protein sample includes: introducing a mixture including the protein sample and an antibody to a nanoDLD array having a plurality of pillars separated by a gap g, wherein the antibody and proteins in the protein sample form antibody-protein complexes having a size that is greater than a size threshold of the nanoDLD array created by the gap g which permits size-based separation of the antibody-protein complexes as the mixture flows through the nanoDLD array; and collecting a purified protein sample containing the antibody-protein complexes from the nanoDLD array. A lab-on-a-chip (LOC) device including the nanoDLD array is also provided.

The present inventions provide methods for detecting and determining protein structures and stability, including heteromeric protein complexes, in biological fluids, such as serum and other bodily fluids. Systems for performing the methods also are provided.

The present inventions provide methods for detecting and evaluating viruses and virus-like particles using A4F combined with Multi-Angle Light Scattering (MALS) and fluorescent (Flr) detectors. Systems for performing the methods also are provided.

The invention relates to analyzing and controlling collection of liquid eluate output from a separation process, in particular by use of a measure of suspended material in the eluate based on a light scattering detection method. Exemplary embodiments include a method of controlling collection of a sample of a liquid eluate output from a separation process. The method includes exposing the liquid eluate to light from a light source; detecting light from the light source scattered by suspended material in the eluate at a detector; and beginning and ending collection of the sample when a measure of the suspended material derived from the detected scattered light enters and leaves a predetermined range.

The present application relates to a flow field-flow fractionation device including a thickness-tapered channel block, and more particularly, to a flow field-flow fractionation device including a thickness-tapered channel block, capable of reducing separation time, improving sample recovery, and exhibiting a flow rate programming effect without a separate equipment, thereby allowing a size range of separation to be expanded.

A fluidic processor device and a wafer including the same, the device including a nanofluidic separator chip including a nanoDLD array, a housing for housing the chip including a top plate disposed on a topside of the chip, a bottom plate disposed on a backside of the chip and fastened to the top plate, and a spacer disposed between the chip and the bottom plate to create a clearance between the chip and the bottom plate for forming a drain space on the backside of the chip.